Author(s): P. Sangeetha, V. Anuradha

Email(s): vanuradha2712@gmail.com

DOI: 10.5958/0974-360X.2020.00414.X   

Address: P. Sangeetha, V. Anuradha*
PG and Research Department of Biochemistry, Mohamed Sathak College Arts and Science, Sholinganallur, Chennai - 600119, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 5,     Year - 2020


ABSTRACT:
The aim of the present study was to investigate the anti-genotoxic effect of methanolic extract of microalgae- Nannochloropsis oculata and also to determine the antibacterial from marine microalga extract in methanol. Genotoxicity studies were conducted for screening of mutagenicity using Ames test, direct plate incorporation method using tester strain of Salmonella typhimurium viz., TA 98 and hence to prove this as an antigenotoxicant. The mutagenicity assay was performed with 3 dose levels (0.312, 1.25 and 5.0mg/ml) using all two tester strains without metabolic activation system (S9 fraction). Inhibition in the background lawn or reduction in revertant colonies was observed at tested dose concentration in two tester strains without metabolic activation system. Further, more works are to be carried out to find the exact mechanism of its Geno protective nature. The methanolic extract of Nannochloropsis oculata was also examined for antimicrobial activity by well- diffusion method. The extract showed high inhibition against the growth of Bacillus Subtilis when compared to the other tested strains. All microorganisms tested are susceptible to the treatment with methanolic extract of Nannochloropsis oculata in a concentration dependent manner.


Cite this article:
P. Sangeetha, V. Anuradha. Antigenotoxicity, Cytogenetic Assay measuring Chromosomal Aberration and Antimicrobial Activity of Methanolic Extract of Microalgae: Nannochloropsis oculate. Research J. Pharm. and Tech 2020; 13(5): 2297-2302. doi: 10.5958/0974-360X.2020.00414.X

Cite(Electronic):
P. Sangeetha, V. Anuradha. Antigenotoxicity, Cytogenetic Assay measuring Chromosomal Aberration and Antimicrobial Activity of Methanolic Extract of Microalgae: Nannochloropsis oculate. Research J. Pharm. and Tech 2020; 13(5): 2297-2302. doi: 10.5958/0974-360X.2020.00414.X   Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-5-44


REFERENCES
1.    Hibberd. Notes on the taxonomy and nomenclature of the algal classes Eustigmatophyceae and Tribophyceae (Synonym Xanthophyceae). Botanical Journal of the Linnean Society. 1981; 82: 93–119.
2.    Pfuhler S, Fellows M, Van Benthem J, Corvi R, Curren R, Dearfield K, Fowler P, Frötschl R, Elhajouji A, Le Hégarat L, Kasamatsu T, Kojima H, Ouédraogo G, Scott A and Speit G. In vitro genotoxicity test approaches with better predictivity: Summary of an IWGT workshop. Mutation Research. 2011; 723: 101-107.
3.    Sugimura T, Sato S, Nagao M, Yahagi T,  Matsushima T, Seino Y. Overlapping of carcinogens and mutagens. In: Fundamental of Cancer Prevention, pp. 191-215; 1976. University Park Press, Baltimore
4.    Ames BN, McCann J and Yamasaki E. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test. Mutation Research 1975; 31: 347–364.
5.    Brusick, D. Genotoxic effects in cultured mammalian cells produced by low pH treatment conditions and increased ion concentrations, Environ. Mutagen 1986; 8: 789-886.
6.    Ames BN, Lee FD and Durston WE. An improved bacterial test system for the detection and classification of mutagens and carcinogens. Proceedings of the National Academy of Science U.S.A. 1973; 70: 2281-2285.
7.    McCann J, Choi E, Yamasaki E and Ames BN. Detection of carcinogens in the Salmonella/microsome test. Assay of 300 chemicals. Proceedings of the National Academy of Science U.S.A. 1975; 72: 5135–5139.
8.    Ames BN. The detection of chemical mutagens with enteric bacteria. In: Chemical Mutagens, Principles and Methods for Their Detection vol. 1 (ed. A. Hollaender), pp. 267-282. Plenum, New York; 1971.
9.    Maron D and Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutation Research. 1983; 113: 173–215
10.    Zeiger E. The Salmonella mutagenicity assay for identification of presumptive carcinogens. In: Handbook of Carcinogen Testing (ed. H.A. Milman, E.K. Weisburger), pp. 83-99. Noyes Publishers, Park Ridge, NJ; 1985.
11.    Monnet DL, Archibald LK, Phillips L, Tenover FC, McGowan JE Jr and Gaynes RP. Antimicrobial use and resistance in eight US hospitals: complexities of analysis and modeling. Infect Control Hospital Epidemiology. 1998; 19: 388-394.
12.    Monnet DL Sakthivelkumar S, Rajendran K and Janarthanan S. Screening of selected marine algae from the coastal Tamil Nadu, South India for antibacterial activity. Asian Pacific Journal of Tropical Biomedical. 2012; 2: S140-S146.
13.    Omar HH, Shiekh HM, Gumgumjee NM, El-Kazan MM and El-Gendy AM. Antibacterial activity of extracts of marine algae from the Red Sea of Jeddah, Saudi Arabia. African Journal of Biotechnology. 2012; 11(71): 13576-13585.
14.    Lazarus S and Bhimba V. Antibacterial activity of marine microalgae against multidrug resistant human pathogens. International Journal of Applied Bioengineering. 2008; 2: 32-34.
15.    Srinivasakumar KP and Rajashekhar M. In vitro studies on bactericidal activity and sensitivity pattern of isolated marine microalgae against selective human bacterial pathogens. Indian Journal of Science and Technology. 2009; 2: 16-23.
16.    Tuney I, Cadirci BH, Unal D and Sukatar A. Antimicrobial activities of the extracts of marine algae from the Coast of Urla (Izmir, Turkey). Turkish Journal of Biology. 2006; 30: 171-175.
17.    Maron D, Ames BN.  Revised methods for the Salmonella mutagenicity test. Mutation Research. 1983; 113: 173–215.
18.    Mendes RL. Supercritical fluid extraction of active compounds from algae. In Martinez, J. L. (Eds). Supercritical fluid extraction of nutraceuticals and bioactive compounds, p. 189-213. United States: CRC Press; 2007.
19.    Vijay DSM, Sirajunnisa AR, Subramaniyan T, Shellomith AS and Tamilselvam K. A green synthesis of antimicrobial compounds from marine microalgae Nannochloropsis oculata. Journal of Coastal Life Medicine.2014; 2: 859-863.
20.    Velioglu YS, Mazza G, Gao L and Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. Journal of Agricultural Food Chemistry. 1998; 46: 4113-4117.
21.    Balasubramanian A, Ramalingam K, Krishana S and Christina AJM. Anti-inflammatory activity of Morus indica Linn. Iran Journal of Pharmacology and Therapeutics. 2005; 4: 13-15.
22.    Hudecova A, Hasplova K, Miadokova E, Magdolenova Z, Rinna A, Galova E, Sevcovicova A, Vaculcikova D, Gregan F and Dusinska M. Cytotoxic and genotoxic effect of methanolic flower extract from Gentiana asclepiadea on COS 1 cells. Neuro Endocrinology Letter. 2010; 31: 21-25.
23.    Mothana RAA, Abdo SAA, Hasson S, Althawab FMN, Alaghbari SAZ and Lindequist U. Antimicrobial, antioxidant and cytotoxic activities and phytochemical screening of some Yemeni medicinal plants. eCAM. 2010; 7: 323-330.
24.    Hasplova K, Hudecova A, Miadokova E, Magdolenova Z, Galova E, Vaculcikova L, Gregan F and Dusinska M.. Biological activity of plant extract isolated from Papaver rhoeas on human lymphoblastoid cell line. Neoplasma 2011; 58: 386-391.
25.    Giiez CM, Emily Waczuk P, Pereira KB, Querol MVM, Rocha JBT and Oliveira LS. In vivo and in vitro Genotoxicity studies of aqueous extract of xanthium spinosum. Brazilian Journal of Pharmaceutical Science. 2012; 48.
26.    Deb DD, Kapoor P, Dighe RP, Padmaja R, Anand MS, D'Souza P Deepak M, Murali B, Agarwal A. In Vitro Safety Evaluation and Anticlastogenic Effect of BacoMind on Human Lymphocytes. Biomedical and Environmental Science. 2008; 21: 7-23.

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