Author(s): Jerine Peter S, Ram Kumar K, Arun Raj N, Sangeetha N, Manisha P, Usha Kumari, Sabina Evan Prince

Email(s): eps674@gmail.com

DOI: 10.5958/0974-360X.2020.00410.2   

Address: Jerine Peter S1, Ram Kumar K1, Arun Raj N1, Sangeetha N1, Manisha P1, Usha Kumari2, Sabina Evan Prince1
1School of Biosciences and Technology, VIT, Vellore-632014, Tamil Nadu, India.
2Faculty of Medicine, AIMST University, Malayasia.
*Corresponding Author

Published In:   Volume - 13,      Issue - 5,     Year - 2020


ABSTRACT:
Guar gum is an herbal drug that is used for treating the diabetes and cancer which is also used as a dietary supplement. It has properties as anti-microbial, anti-tumor, and anti-inflammatory. Hyperuricemia or Gout is an acute inflammation that can be found in joints with the indications of redness and swollen knees. The aim of our study is to know the potential effect of Cyamopsis tetragonoloba through in vitro and in silico analysis. The antioxidant activities like catalase, peroxidase, DPPH assay and total phenolic content were evaluated in the aqueous, ethanolic and methanolic extract of Cyamopsis tetragonoloba seed. Ligands of Cyamopsis tetragonoloba seed were obtained from the literature and the 3d structure of the ligands were retrieved from Pubchem online server followed by Corina molecular network. Receptors responsible for causing gouty arthritis were obtained from RCSB protein data bank in the format of 3d structure. Patch dock online serve was used to perform the docking analysis which was then visualized and analyzed in PyMOL. Extract of Cyamopsis tetragonoloba seed shown good antioxidant potential in which aqueous extract was observed to show better antioxidant activity. Docked complex with Hexopyranosyl hexopyranoside has shown better interaction with higher binding residues than the other ligands. Further, in vivo studies can be carried out to known the potential activity of this ligand against gouty arthritis.


Cite this article:
Jerine Peter S, Ram Kumar K, Arun Raj N, Sangeetha N, Manisha P, Usha Kumari, Sabina Evan Prince. Evaluation of the Antioxidant and Potential binding affinity of Cyamopsis tetragonoloba seed against the receptor responsible for Gouty Arthritis. Research J. Pharm. and Tech 2020; 13(5): 2275-2281. doi: 10.5958/0974-360X.2020.00410.2

Cite(Electronic):
Jerine Peter S, Ram Kumar K, Arun Raj N, Sangeetha N, Manisha P, Usha Kumari, Sabina Evan Prince. Evaluation of the Antioxidant and Potential binding affinity of Cyamopsis tetragonoloba seed against the receptor responsible for Gouty Arthritis. Research J. Pharm. and Tech 2020; 13(5): 2275-2281. doi: 10.5958/0974-360X.2020.00410.2   Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-5-40


7.    REFERENCE:
1.    Prem D, Singh S, Gupta PP, Singh J, Kadyan SPS. Callus induction and de novo regeneration from callus in Guar (Cyamopsis tetragonoloba). Plant Cell Tissue Organ Cult 2005;80:209–14.
2.    Whistler RL, Hymowitz T. Guar: Agronomy, Production, Industrial Use, and Nutrition. West Lafayette, Ind: Purdue University Press; 1979. 124 p.
3.    Mudgil D, Barak S, Khatkar BS. Guar gum: processing, properties and food applications—A Review. J Food Sci Technol 2014;51:409–18.
4.    Harju EJ, Larmi TKJ. Effect of Guar Gum Added to the Diet of Patients with Duodenal Ulcer.
5.    Elias EJ, Anil S, Ahmad S, Daud A. Colon targeted curcumin delivery using guar gum. Nat Prod Commun 2010;5:915–8.
6.    Nasry MR, Abo-Youssef AM, Abd El-Latif HA. Anti-diabetic activity of the petroleum ether extract of Guar gum in streptozotocin-induced diabetic rats: A comparative study. Beni-Suef Univ J Basic Appl Sci 2013;2:51–9.
7.    Mukhtar HM, Ansari SH, Bhat ZA, Naved T. Antihyperglycemic Activity of Cyamopsis tetragonoloba. Beans on Blood Glucose Levels in Alloxan-Induced Diabetic Rats. Pharm Biol 2006;44:10–3.
8.    Gamal-Eldeen AM, Amer H, Helmy WA. Cancer chemopreventive and anti-inflammatory activities of chemically modified guar gum. Chem Biol Interact 2006;161:229–40.
9.    Hamdani AM, Wani IA, Bhat NA, Siddiqi RA. Effect of guar gum conjugation on functional, antioxidant and antimicrobial activity of egg white lysozyme. Food Chem 2018;240:1201–9.
10.    Santos R, Gomes D, Macedo H, Barros D, Tibério C, Veiga AS, et al. Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolates. J Med Microbiol 2016;65:1092–9.
11.    P M, J R, R M, S C. In vitro free radical scavenging and antimicrobial activity of Cyamopsis tetragonoloba L. J Pharmacogn Phytochem 2015;4:102–6.
12.    Farah N, Hassan I. Analytical Characterization of Guar and Guar Gum Produced in Sindh , Pakistan [Internet]. 2017.
13.    Saeed S, Mosa-Al-Reza H, Fatemeh AN, Saeideh D. Antihyperglycemic and antihyperlipidemic effects of guar gum on streptozotocin-induced diabetes in male rats. Pharmacogn Mag 2012;8:65–72.
14.    Yeo S-K, Ooi L-G, Lim T-J, Liong M-T. Antihypertensive Properties of Plant-Based Prebiotics. Int J Mol Sci 2009;10:3517–30.
15.    Bhattacharya A. Antipyretic, Anti-Inflammatory and Analgesic Effects of Leaf Extract of Drumstick Tree. J Young Pharm 2014;6:20–4.
16.    Richette P, Bardin T. Gout. The Lancet 2010;375:318–28.
17.    Sabina EP, Nagar S, Rasool M. A role of piperine on monosodium urate crystal-induced inflammation--an experimental model of gouty arthritis. Inflammation 2011;34:184–92.
18.    An J, Yang H-J, Park K, Lee J, Kim B-W. Reparatory and preventive effects of oriental herb extract mixture (OHEM) on hyperuricemia and gout. Food Sci Biotechnol 2010;19:517–24.
19.    Harris MD, Siegel LB, Alloway JA. Gout and Hyperuricemia. Am Fam Physician 1999;59:925.
20.    Ragab G, Elshahaly M, Bardin T. Gout: An old disease in new perspective – A review. J Adv Res 2017;8:495–511.
21.    Marcolongo R, Marinello E, Pompucci G, Pagani R. The role of xanthine oxidase in hyperuricemic states. Arthritis Rheum 1974;17:430–8.
22.    Dalbeth N, Stamp LK, Merriman TR. The genetics of gout: towards personalised medicine? BMC Med 2017;15:108.
23.    Lück H. Catalase. In: Bergmeyer H-U, editor. Methods of Enzymatic Analysis Academic Press; 1965. p. 885–94.
24.    Reddy KP, Subhani SM, Khan PA, Kumar KB (Khallikote C. Effect of light and benzyladenine on dark-treated growing rice (Oryza sativa) leaves, 2: Changes in peroxidase activity. Plant Cell Physiol Jpn 1985;
25.    Blois MS. Antioxidant Determinations by the Use of a Stable Free Radical. Nature 1958;181:1199–200.
26.    Singleton VL, Orthofer R, Lamuela-Raventós RM. [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In: Methods in Enzymology Academic Press; 1999. p. 152–78. (Oxidants and Antioxidants Part A; vol. 299).
27.    Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, et al. The Protein Data Bank. Nucleic Acids Res 2000;28:235–42.
28.    Wass MN, Kelley LA, Sternberg MJE. 3DLigandSite: predicting ligand-binding sites using similar structures. Nucleic Acids Res 2010;38:W469–73.
29.    Surendran S, Vijayalakshmi K. GC-MS analysis of phytochemicals in Cyamopsis tetragonoloba fruit and Cyperus rotundus rhizome. Int J Pharmacogn Phytochem Res 2011;3:102–6.
30.    Duhovny D, Nussinov R, Wolfson HJ. Efficient Unbound Docking of Rigid Molecules. In: Guigó R, Gusfield D, editors. Algorithms in Bioinformatics Springer Berlin Heidelberg; 2002. p. 185–200. (Lecture Notes in Computer Science).
31.    Iwase T, Tajima A, Sugimoto S, Okuda K, Hironaka I, Kamata Y, et al. A Simple Assay for Measuring Catalase Activity: A Visual Approach. Sci Rep 2013;3:3081.
32.    Rajeswari VD, Lavinya BU, Akalya JL, Srujana KSL, Sabina EP. Appraisal of the In Vitro antibacterial and antioxidant potential of the leaf extracts of Cadaba fruticosa. Asian J Pharm Clin Res 2014;7:118–20.
33.    Balasundram N, Sundram K, Samman S. Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chem 2006;99:191–203.
34.    Castro RR, Feitosa JPA, da Cunha PLR, da Rocha FAC. Analgesic activity of a polysaccharide in experimental osteoarthritis in rats. Clin Rheumatol 2007;26:1312–9.
35.    Pucker AD, Ng SM, Nichols JJ. Over the counter (OTC) artificial tear drops for dry eye syndrome. Cochrane Database Syst Rev 2016;
36.    Ko G-A, Cho SK. Phytol suppresses melanogenesis through proteasomal degradation of MITF via the ROS-ERK signaling pathway. Chem Biol Interact 2018; 286:132–40.
37.    Miller ER, Pastor-Barriuso R, Dalal D, Riemersma RA, Appel LJ, Guallar E. Meta-analysis: high-dosage vitamin E supplementation may increase all-cause mortality. Ann Intern Med 2005;142:37–46.

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