Archana R Dhole, V. C. Yeligar
email@example.com , firstname.lastname@example.org
Archana R Dhole1, Dr. V. C. Yeligar2
1Rajarambapu College of Pharmacy, Kasegaon, Tal- Walva, Dist- Sangli, Pin- 415409.
2Principal, Sarojini College of Pharmacy, Kolhapur. Tal-Karvir, Dist-Kolhapur.
Volume - 13,
Issue - 4,
Year - 2020
Objective: Evaluate the abilities of plant extracts used for urolithiasis in –vitro models can be served as an aid in the evaluation of novel treatments for urolithiasis. Methods: The powdered material (1.5 kg) was subjected for extraction with various solvent in soxhlet apparatus and microwave assisted extraction based on increasing polarity in order of petroleum ether (60-80°C), chloroform, ethyl acetate and ethanol, aqueous. Ethanolic extracts of whole plants was assessed for its protective and curative activity in urolithiasis. In vitro experiments (nucleation, aggregation and microscopic assay's) were performed to test the anti-urolithiasis activity of microwave assisted extracted Cissampelos pareira Linn, Fragaria vesca Linn and Biophytum sensitivum Linn extracts. These ethanol extracts were tested at increasing concentration of 100, 200, 400 ug/ml and compared with the standard drug cystone. Results: The extracts were tested for the inhibition percentage of anti-urolithiasis potential against preformed calcium oxalate crystal. The % inhibition rates of nucleation of caOx by cystone is 44.776%, 54.526%, 75.31% at 100 (µg/mL), 200 (µg/mL), 400 (µg/mL) respectively, while in Cissampelous pareira L (CP), Fragarica vesca (FV) L, Biophytum sensitivum L (BS) shows high % inhibition at 400 (µg/mL) i.e 68.556, 69.54±1.38, 64.630±0.66. combination of Extract shows higher % inhibition i.e 79.813±0.42 which gives more effective than standard cystone drug. The results of ethanol extracts of Cissampelos pareira Linn (49.98%), Fragaria vesca Linn (46.406%) and Biophytum sensitivum Linn (46.406%) on Aggregation assy were found to be significant and the ethanol leaf extract showed considerable inhibition compared to standard cystone (65.096%) drug. Combination of extract with 400ug/ml shows best result i.e 89.353%. Conclusion: Cissampelos pareira linn, Fragaria vesca linn and Biophytum sensitivum linn extracts in combination were effective on urolithiasis.
Cite this article:
Archana R Dhole, V. C. Yeligar. In- vitro Protective and Curative effect of Cissampelos pareira Linn, Fragaria vesca Linn and Biophytum sensitivum Linn extracts on Urolithiasis. Research J. Pharm. and Tech. 2020; 13(4):1841-1846. doi: 10.5958/0974-360X.2020.00332.7
1. Butt AJ. Historical survey. In Etiological factors in renal lithiasis. ED. Butt AJ, Springfield, Illinis, CC Thomus., 1956 : 3.
2. Suman Singh, Nishteswar K Review on Cissampelos Pareira and Cyclea Peltata (Patha Dwaya) Phyto-Pharmacological Perspectives International Journal of Ayurvedic Medicine, 2013; 4 (4) : 282-289.
3. Lin YL, Wang WY. Chemical constituents of Biophytum sensitivum. Chin Pharm Jr., 2003; 55 : 71-5.
4. "Fragaria vesca" PLANTS Database. United States Department of Agriculture; Natural Resources Conservation Service. 2015. Retrieved 2015-06-16.
5. Bhattacharya. J.S. Rana A.S. Phytochemical investigation and pharmacognostic standardization of Cissampelos pareira stem, 2012; 6(4): 2: 299-303.
6. Pal T.K. et al., Quantification of total flavonoid content and antioxidant activity in comparison to a reference flavonoid as in vitro quality evaluation parameter for assessing bioactivity of biomarkers in herbal extracts or formulations, JPR: BioMedRx: An International Journal, 2013;1(8):757-766.
7. Yella. V.R. Effect of ethanolic extract of Fragaria vesca on serum glucose levels and body weight in diet induced obese rats. International Journal of Pharmacological Research, 2014; 5 (10): 236-240.
8. Agarwal. S.S. Herbal drug extract technology, University press India, Hyderabad, 2012; 658-659.
9. Agarwal, A. S. Tandon, S. K. Singla, C. T. Diminution of Oxalate Induced Renal Tubular Epithelial Cell Injury and inhibition of calcium oxalate crystallization in vitro by aqueous extract of Tribulus terrestris. International Braz J Urol, July - August, 2010; 36 (4): 480-489.