Author(s): Ola Mahmoud Younes, Fida Am Ali, Zaid Al Assaf


DOI: 10.5958/0974-360X.2020.00304.2   

Address: Ola Mahmoud Younes1*, Fida Am Ali2, Zaid Al Assaf3
1PhD Student, Department of Analytical and Food Chemistry, Faculty of Pharmacy, Damascus University, Damascus, Syria.
2Professor Assistant, Department of Analytical and Food Chemistry, Faculty of Pharmacy, Damascus University, Damascus, Syria.
3Professor, Department of Analytical and Food Chemistry, Faculty of Pharmacy, Damascus University, Damascus, Syria.
*Corresponding Author

Published In:   Volume - 13,      Issue - 4,     Year - 2020

This paper presents the results of HPLC enantioseparation of carvedilol using HPLC by two different methods the first one is reversed phase HPLC (RP-HPLC), In which carboxy methyl-??- cyclodextrin (CM-??-CD) is used as chiral mobile phase additive the second one, which is common used, depends on using chiral stationary phase (polysaccharide type, Chiralcel OD-R column, 250 mm×4.6 mm). In mobile phase chiral additive method, the highest resolution was achieved using Knauer C18 column (250 mm×4.6 mm) and mobile phase made up of a mixture of aqueous solution [pH=4.4, (2.3g/l CM-??-CD)], methanol and acetonitrile with a volumetric ratio of (56.8/ 30.7/12.5) v/v%, flow rate of 0.7mL/min, and column temperature was set at 40°.In chiral stationary phase method ,the mobile phase consisted of acetonitrile, isopropanol, and diethyl amine with a volumetric ratio of (95/ 5/0.1) v/v%, flow rate of 1 ml/min at room temperature. The effects of different conditions on the enantioseparation of carvedilol were investigated. Both methods showed good resolution of carvedilol enantiomers. But the first new one is very inexpensive comparing with the second one.

Cite this article:
Ola Mahmoud Younes, Fida Am Ali, Zaid Al Assaf. Separation of Carvedilol Enantiomers using HPLC by two Different Methods: Mobile Phase Chiral Additive and Chiral Stationary Phase. Research J. Pharm. and Tech. 2020; 13(4): 1679-1683. doi: 10.5958/0974-360X.2020.00304.2

1.    Basaveswara Rao M. Vnagendrakumar A. V. D, Yedukondalu M, Raman B.V. New validated RP–HPLC method for the estimation of carvedilol in pharmaceutical formulation. International Journal of Pharmacy and Pharmaceutical Sciences, 2012; 4(2): ISSN- 0975-1491.
2.    Gabriel Hancua, Anca Cârje, Ileana Iuga, Ibolya Fülöpc and Zoltán-István Szabó. Cyclodextrine Screening for the Chiral Separation of Carvedilol by Capillary Electrophoresis, Iranian Journal of Pharmaceutical Research. 2015;14 (2): 425-433.
3.    Sylwia Magiera, Weronika Adolf, Irena Baranowska. Simultaneous chiral separation and determination of carvedilol and 5’-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection. Cent. Eur. J. Chem. 2013; 11(12) :2076-2087.
4.    B. Koppe N H Oefer, U. Epperle I N, B. Christian , B. Lln,Y. J,Y. C H En. Separation of enantiomers of drugs by capillary electrophoresis III. β-cyclodextrin as chiral solvating agent, Journal of Chromatography A. 1996; 735: 3 3 3 - 3 4 3.
5.    Samin Hamidi, Somaieh Soltani and Abolghasem Jouyban. A dispersive liquid–liquid microextraction and chiral separation of carvedilol in human plasma using capillary electrophoresis. Bioanalysis .2015; 7(9):1107–1117.
6.    Monica Budău, Gabriel Hancu, Zoltán István Szabó, Hajnal Kelemen, Aura Rusu, Daniela Lucia Muntean, Anca Gabriela Cârje. Captisol® AS chiral selector in capillary electrophoresis of non-acidic drugs. J. Chil. Chem. Soc.2017;62 (3) .3566-3571
7.    Radu-Cristian Moldovan, Gabriel-Sorin Dascăl, Valentin Mirel, Ede Bodoki, Radu Oprean. Chiral separation of 16 beta-blockers on immobilized polysaccharide chiral stationary phases. Farmacia. 2015 ;6 (63):909-912.
8.    K. Hrobon ˇOva ´1, J. Lehotay1, R. C ˇ IZ ˇMa ´Rikova. HPLC enantioseparation of potential β-blockers of the aryloxyaminopropanol type Study of the mechanism of enantioseparation, part VIII, Pharmazie .2004;59: 828–832 .
9.    Ettireddy Swetha, Chandupatla Vijitha, Ciddi Veeresham. HPLC Method Development and Validation of S(-)-Carvedilol from API and Formulations. American Journal of Analytical Chemistry, 2015; 6, 437-445
10.    Massimiliano Pio di Cagno. The Potential of Cyclodextrins as Novel Active Pharmaceutical Ingredients: A Short Overview. Molecules. 2017;22 (1): 1-14.
11.    Yangfeng Peng, Quan Sophia He, and Jiang Cai. Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-𝛽-Cyclodextrin as a Chiral Mobile Phase Additive. International Journal of Analytical Chemistry, 2016, Article ID 1231386, 7 pages.
12.    Surendra Dutt Sharma, Gaurav Singh. Enantioseparation of Nadifloxacin by High performance liquid Chromatography, Advances in Analytical Chemistry. 2012; 2(4): 25-31.
13.    Tamas Sohajda, Zoltan Szak ´ Acs, Lajos Szente, Bela Nosz ´ Al, Szabolcs Ben. Chiral recognition of imperanene enantiomers by various cyclodextrins: A capillary electrophoresis and NMR spectroscopy study. Electrophoresis. 2012;33;1458–1464.
14.    Li L, Li X, Luo Q, You T. A comprehensive study of the enantioseparation of chiral drugs by cyclodextrin using capillary electrophoresis combined with theoretical approaches. Talanta. 2015;142: 28-34.
15.    Xiaoyu Hu, Xin Guo, Shuo Sun, Bolin Zhu, Jia Yu, Xingjie Guo. Enantioseparation of nine indanone and tetralone derivatives by HPLC using carboxymethyl‐β‐cyclodextrin as the mobile phase additive. Chirality .2017; 29: 38–47.

Recomonded Articles:

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Mukta D. Naykode, Durgacharan A. Bhagwat, Swapnil D. Jadhav, Harinath N. More

DOI: 10.5958/0974-360X.2017.00133.0         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Katare Varsha B., Bhutkar M. A., Kumbhar A. P., Pol S. K., Katare P. B.

DOI: Not Available         Access: Open Access Read More

Author(s): Krishnendu Acharya*, Somanjana Khatua, Salman Sahid

DOI: Not Available         Access: Open Access Read More

Author(s): S. Poongothai, C.M. Karrunakaran, R. Ilavarasan

DOI: Not Available         Access: Open Access Read More

Author(s): Oruganti Sai Koushik, V. Himaja, P. Srinivasa Babu, Ramadoss Karthikeyan

DOI: 10.5958/0974-360X.2015.00298.X         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles