Author(s): Mahadeva Rao U.S., S Akbar Kausar, R. Babu Janarthanam, Sinouvassane Djearamane, S. Suresh Kumar

Email(s): ,

DOI: 10.5958/0974-360X.2020.00269.3   

Address: Mahadeva Rao U.S. 1*, S Akbar Kausar2, R. Babu Janarthanam3, Sinouvassane Djearamane4, S. Suresh Kumar5,6,7
1Professor, Head of Biochemistry Unit, Faculty of Medicine, Universiti Sultan Zainal Abidin, Kuala Terengganu, Malaysia.
2President, Al-Kausar Tibb –E–Nabuvi Research Foundation Trust, Vaniyambadi, India.
3Associate Professor, Department of Biotechnology, Thiruvalluvar University, Vellore, India.
4Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia.
5Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia 43400, UPM Serdang Selangor, Malaysia.
6Genetics and Regenerative Medicine Research Centre, Universiti Putra Malaysia 43400, UPM Serdang, Selangor, Malaysia.
7Institute of Bioscience, Universiti Putra Malaysia,Serdang 43400 UPM, Selangor, Malaysia. *Corresponding Author

Published In:   Volume - 13,      Issue - 3,     Year - 2020

Objective: The study was performed to assess the antidiabetic properties of a polyherbal formulation (Kasini ashshifa) comprising of the thirteen herbals in HFD fed-low dose STZ induced experimental type 2 diabetes (T2D) in rats. Animal grouping: Healthy, male Wistar rats were studied. The rodents were separated into four groups. Group I used as positive control and Group II as negative control. Group III was diabetic rats treated with Kasini ashshifa. Group IV was diabetes rats administered with metformin. Methodology: Body weight, food and fluid intake were recorded periodically. OGTT was performed. The biochemical analysis accomplished includes fasting blood glucose, C-peptide, HbA1c, plasma insulin, and urine sugar. Insulin resistance (IR), ß-cell function and insulin sensitivity were also manipulated from HOMA-IR and HOMA ß-cell function. Results: The findings of the experiment distinctly indicate that the polyherbal formulation has significantly reduced the readings of fasting blood glucose (FBG) and HbA1c. Kasini ashshifa improves the plasma insulin and C-peptide levels. From the arithmetic data, Kasini ashshifa reduces the IR and ameliorates ß-cell function and insulin sensitivity. The antidiabetic efficacy was more noticeable in rats administered with Kasini ashshifa. Conclusion: The results obtained postulated that the Kasini ashshifa possess significant antidiabetic properties indicating the synergistic effect of herbals.

Cite this article:
Mahadeva Rao U.S., S Akbar Kausar, R. Babu Janarthanam, Sinouvassane Djearamane, S. Suresh Kumar. Biochemical Evaluation of Antidiabetic Effect of Kasini ashshifa, a Polyherbal formulation in High Fat Diet Fed- Low Dose STZ Induced Diabetes in Rats. Research J. Pharm. and Tech 2020; 13(3):1474-1482. doi: 10.5958/0974-360X.2020.00269.3

Mahadeva Rao U.S., S Akbar Kausar, R. Babu Janarthanam, Sinouvassane Djearamane, S. Suresh Kumar. Biochemical Evaluation of Antidiabetic Effect of Kasini ashshifa, a Polyherbal formulation in High Fat Diet Fed- Low Dose STZ Induced Diabetes in Rats. Research J. Pharm. and Tech 2020; 13(3):1474-1482. doi: 10.5958/0974-360X.2020.00269.3   Available on:


1.       Rao USM. Effects of Quercitrin on Diabetic Physiological Criterions and Hematological Parameters Studied in Diabetic Rats. International Medical Journal. 201, 26 (4), 266-269.

2.       International Diabetes Federation. 8th Edition; 2018

3.       Candib LM. Obesity and diabetes in vulnerable populations: reflection on proximal and distal causes. The Annals of Family Medicine. 2007 Nov 1;5(6):547-56.

4.       Piero MN, Nzaro GM, Njagi JM. Diabetes mellitus-a devastating metabolic disorder. Asian Journal of Biomedical and Pharmaceutical Sciences. 2015 Jan 1;5(40):1.

5.       Parasuraman S, Thing GS, Dhanaraj SA. Polyherbal formulation: Concept of ayurveda. Pharmacognosy Reviews. 2014 Jul;8(16):73.

6.       Abbas ZK, Saggu S, Sakeran MI, Zidan N, Rehman H, Ansari AA. Phytochemical, antioxidant and mineral composition of hydroalcoholic extract of chicory (Cichorium intybus L.) leaves. Saudi Journal of Biological Sciences. 2015 May 1;22(3):322-6.

7.       Chabra A, Monadi T, Azadbakht M, Haerizadeh SI. Ethnopharmacology of Cuscuta epithymum: A comprehensive review on ethnobotany, phytochemistry, pharmacology and toxicity. Journal of Ethnopharmacology. 2018 Dec 3.

8.       Rather MA, Dar BA, Sofi SN, Bhat BA, Qurishi MA. Foeniculum vulgare: A comprehensive review of its traditional use, phytochemistry, pharmacology, and safety. Arabian Journal of Chemistry. 2016 Nov 1;9:S1574-83.

9.       Bais S, Gill NS, Rana N, Shandil S. A phytopharmacological review on a medicinal plant: Juniperus communis. International Scholarly Research Notices. 2014;2014.

10.    Amiri MS, Joharchi MR, TaghavizadehYazdi ME. Ethno-medicinal plants used to cure jaundice by traditional healers of Mashhad, Iran. Iranian Journal of Pharmaceutical Research: IJPR. 2014;13(1):157.

11.    Kostova I, Dinchev D. Saponins in Tribulus terrestris–chemistry and bioactivity. Phytochemistry Reviews. 2005 Jul 1;4(2-3):111-37.

12.    Das RA, Sahu JA, Gupta RN. Potentiative Activity of Cucurbita Maxima Seed Extract With Beta Vulgaris and Smilax Regelii Root Extract To Reduce Extra Fats From The Body. International Journal of Pharmaceutical Sciences and Research. 2010;1:57-62.

13.    Khan LU, Khan RA, Ahmed M, Khan WU, Khan MW. Phytochemical investigation and pharmacological activities of Tephrosia purpurea (L.) Pers. Bark. Int. J. Biol. Biotech. 2017;14(2):173-8.

14.    Bhatia DK, Sharma AK, Pathania PC, Khanduri NC. Antifertility effects of crude different of Adiantum lunulatum Burm. on Reproductive Organs of male albino rats. InBiological Forum—An International Journal 2010 (Vol. 2, No. 2, pp. 88-93).

15.    Ivanova D, Vankova D, Nashar M. Agrimonia eupatoria tea consumption in relation to markers of inflammation, oxidative status and lipid metabolism in healthy subjects. Archives of Physiology and Biochemistry. 2013 Feb 1;119(1):32-7.

16.    Khadri A, Neffati M, Smiti S, Falé P, Lino AR, Serralheiro ML, Araújo ME. Antioxidant, antiacetylcholinesterase and antimicrobial activities of Cymbopogon schoenanthus L. Spreng (lemon grass) from Tunisia. LWT-Food Science and Technology. 2010 Mar 1;43(2):331-6.

17.    Albayrak S, Aksoy A. Evaluation of antioxidant and antimicrobial activities of two endemic anthemis species in Turkey. Journal of Food Biochemistry. 2013 Dec;37(6):639-45.

18.    Cemek M, Kağa S, Şimşek N, Büyükokuroğlu ME, Konuk M. Antihyperglycemic and antioxidative potential of Matricaria chamomilla L. in streptozotocin-induced diabetic rats. Journal of Natural Medicines. 2008 Jul 1;62(3):284-93.

19.    K.Srinivasan, B.Viswanad, L.Asrat, C.L.Kaul, and P.Ramarao, “Combination of high-fatdiet-fed and low-dose streptozotocin treated rat: a model for type 2 diabetes and pharmacological screening,”Pharmacological Research, Vol.52, No.4, Pp.313–320, 2005.

20.    Ericka Nevarez, Norelia Ordonez-Castillo, Rebekah Spainhour, and Yass Kobayashi. Treatment with Streptozotocin (STZ) causes hypoglycemia and alters the stability of reference genes for real-time PCR analysis in the liver of channel catfish. The FASEB Journal 2017 31:1_Supplement, 1014.3-1014.3

21.    Nayak SS, Pattabiraman TN. A new colorimetric method for the estimation of glycosylated hemoglobin. Clinica Chimica Acta. 1981 Feb 5;109(3):267-74.

22.    Faradji RN, Monroy K, Messinger S, Pileggi A, Froud T, Baidal DA, Cure PE, Ricordi C, Luzi L, Alejandro R. Simple Measures to Monitor β‐Cell Mass and Assess Islet Graft Dysfunction. American Journal of Transplantation. 2007 Feb;7(2):303-8.

23.    Lenzen S. The mechanisms of alloxan-and streptozotocin-induced diabetes. Diabetologia. 2008 Feb 1;51(2):216-26.

24.    Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiological Research. 2001 Jan 1;50(6):537-46.

25.    Reed MJ, Meszaros K, Entes LJ, Claypool MD, Pinkett JG, Gadbois TM, Reaven GM. A new rat model of type 2 diabetes: the fat-fed, streptozotocin-treated rat. Metabolism-Clinical and Experimental. 2000 Nov 1;49(11):1390-4.

26.    Prentki M, Nolan CJ. Islet β cell failure in type 2 diabetes. The Journal of Clinical Investigation. 2006 Jul 3;116(7):1802-12.

27.    Tabák AG, Jokela M, Akbaraly TN, Brunner EJ, Kivimäki M, Witte DR. Trajectories of glycaemia, insulin sensitivity, and insulin secretion before diagnosis of type 2 diabetes: an analysis from the Whitehall II study. The Lancet. 2009 Jun 27; 373(9682): 2215-21.

28.    Dankner R, Shanik MH, Keinan-Boker L, Cohen C, Chetrit A. Effect of elevated basal insulin on cancer incidence and mortality in cancer incident patients: the Israel GOH 29-year follow-up study. Diabetes Care. 2012 Jul 1;35(7):1538-43.

29.    Skovsø S. Modeling type 2 diabetes in rats using high fat diet and streptozotocin. Journal of Diabetes Investigation. 2014 Jul;5(4):349-58.

30.    West E, Simon OR, Morrison EY. Streptozotocin alters pancreatic beta-cell responsiveness to glucose within six hours of injection into rats. The West Indian Medical Journal. 1996 Jun; 45(2):60-2.

31.    Butler AE, Janson J, Bonner-Weir S, Ritzel R, Rizza RA, Butler PC. β-cell deficit and increased β-cell apoptosis in humans with type 2 diabetes. Diabetes. 2003 Jan 1;52(1):102-10.

32.    Atkinson MA. Pancreatic biopsies in type 1 diabetes: revisiting the myth of Pandora’s box. Diabetologia. 2014 Apr 1;57(4):656-9.

33.    Li W, Zhang M, Gu J, Meng ZJ, Zhao LC, Zheng YN, Chen L, Yang GL. Hypoglycemic effect of protopanaxadiol-type ginsenosides and compound K on Type 2 diabetes mice induced by high-fat diet combining with streptozotocin via suppression of hepatic gluconeogenesis. Fitoterapia. 2012 Jan 1;83(1):192-8.

34.    Andallu B, Varadacharyulu NC. Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats. Clinica Chimica Acta. 2003 Dec 1;338(1-2):3-10.

35.    Yanardag R, Ozsoy-Sacan O, Ozdil S, Bolkent S. Combined effects of vitamin C, vitamin E, and sodium selenate supplementation on absolute ethanol-induced injury in various organs of rats. International Journal of Toxicology. 2007 Nov;26(6):513-23.

36.    Fournier A. Diagnosing Diabetes: A Practitioner's Plea: Keep It Simple. Journal of General Internal Medicine. 2000 Aug;15(8):603.

37.    Raju SM, Raju B. Regulation of Blood Glucose and Diabetes. Illustrated medical biochemistry. 2nd Edition. Jaypee Brothers Medical Publishers Ltd. New Delhi. India. 2010:445-56.

38.    Dahlgren G, Whitehead M. Levelling Up: Strategies for Tackling Social Inequalities in Health. Venice, Italy: WHO-EURO Office for Investment for Health and Development; 2006.

39.    Matsuda M, DeFronzo RA. Insulin sensitivity indices obtained from oral glucose tolerance testing: comparison with the euglycemic insulin clamp. Diabetes Care. 1999 Sep 1;22(9):1462-70.

40.    Manell E, Hedenqvist P, Svensson A, Jensen-Waern M. Establishment of a refined oral glucose tolerance test in pigs, and assessment of insulin, glucagon and glucagon-like peptide-1 responses. PloS one. 2016 Feb 9;11(2):e0148896.

41.    Chandalia HB, Krishnaswamy PR. Glycated hemoglobin. Current Science. 2002 Dec 25:1522-32.

42.    McCance DR, Pettitt DJ, Hanson RL, Jacobsson LT, Bennett PH, Knowler WC. Glucose, insulin concentrations and obesity in childhood and adolescence as predictors of NIDDM. Diabetologia. 1994 Jun 1;37(6):617-23.

43.    Davidson MB, Schriger DL, Peters AL, Lorber B. Relationship between fasting plasma glucose and glycosylated hemoglobin: potential for false-positive diagnoses of type 2 diabetes using new diagnostic criteria. JAMA. 1999 Apr 7;281(13):1203-10.

44.    Fitzgibbons JF, Koler RD, Jones RT. Red cell age-related changes of hemoglobins AIa+ b and AIc in normal and diabetic subjects. The Journal of Clinical Investigation. 1976 Oct 1;58(4):820-4.

45.    Bunn HF, Gabbay KH, Gallop PM. The glycosylation of hemoglobin: relevance to diabetes mellitus. Science. 1978 Apr 7;200(4337):21-7.

46.    Bunn HF, Haney DN, Kamin S, Gabbay KH, Gallop PM. 21. The biosynthesis of human hemoglobin A1c. Slow glycosylation of hemoglobin in vivo. J. Clin. Invest. 1976;57:1652-9.

47.    IM S. Adler AI. Neil HA. Matthews DR. Manley SE. Cull CA. Hadden D. Turner RC. Holman RR. Association of glycaemia with macrovascular and microvascular complications of type 2 diabetes (UKPDS 35): prospective observational study. BMJ. 2000;321(7258):405-12.

48.    Petchi RR, Vijaya C, Parasuraman S. Antidiabetic activity of polyherbal formulation in streptozotocin–nicotinamide induced diabetic Wistar rats. Journal of Traditional and Complementary Medicine. 2014 Apr 1;4(2):108-17.

49.    Mulvey CK, McNeill AM, Girman CJ, Churchill TW, Terembula K, Ferguson JF, Shah R, Mehta NN, Qasim AN, Rickels MR, Reilly MP. Differential associations of oral glucose tolerance test–derived measures of insulin sensitivity and pancreatic β-cell function with coronary artery calcification and microalbuminuria in type 2 diabetes. Diabetes Care. 2014 Jan 1;37(1):124-33.

50.    Wahren J, Kallas Å, Sima AA. The clinical potential of C-peptide replacement in type 1 diabetes. Diabetes. 2012 Apr 1;61(4):761-72.

51.    Massi-Benedetti M, Orsini-Federici M. Treatment of type 2 diabetes with combined therapy: what are the pros and cons?. Diabetes Care. 2008 Feb 1;31(Supplement 2):S131-5.

52.    Hills CE, Brunskill NJ, Squires PE. C-peptide as a therapeutic tool in diabetic nephropathy. American Journal of Nephrology. 2010;31(5):389-97.

53.    Matthews DR. lP. Hosker, AS. Rudenski, BA Naylor, DF Treacher, and RC Turner, Homeostasis model assessment: insulin resistance and beta-cell jimction from fasting plasma glucose and insulin concentrations in man. Diabetologia. 1985;28(7):412-9.

54.    Farideh Rezaei Abhari, Maryam Ghanbari Andarieh, Asadollah Farokhfar, and Soleiman Ahmady, “Estimating Rate of Insulin Resistance in Patients with Preeclampsia Using HOMA-IR Index and Comparison with Nonpreeclampsia Pregnant Women,” BioMed Research International, vol. 2014, Article ID 140851, 6 pages, 2014.

55.    Robertson RP, Halter JB, Porte D. A role for alpha-adrenergic receptors in abnormal insulin secretion in diabetes mellitus. The Journal of Clinical Investigation. 1976 Mar 1;57(3):791-5.

56.    Ward WK, Bolgiano DC, McKnight B, Halter JB, Porte D. Diminished B cell secretory capacity in patients with noninsulin-dependent diabetes mellitus. The Journal of Clinical Investigation. 1984 Oct 1;74(4):1318-28.

57.    Muthusamy VS, Anand S, Sangeetha KN, Sujatha S, Arun B, Lakshmi BS. Tannins present in Cichorium intybus enhance glucose uptake and inhibit adipogenesis in 3T3-L1 adipocytes through PTP1B inhibition. Chemico-Biological Interactions. 2008 Jul 10;174(1):69-78.

58.    Ganapaty SE, Ramaiah MA, Yasaswini KA, Kumar CR. Determination of total phenolic, flavonoid, alkaloidal contents and in vitro screening for hepatoprotective activity of Cuscuta epithymum (L) whole plant against CCl4 induced liver damage animal model. Int J Pharm Pharm Sci. 2013;5(4):738-42.

59.    El-Soud N, El-Laithy N, El-Saeed G, Wahby M, Khalil M, Morsy F, Shaffie N. Antidiabetic activities of Foeniculum vulgare Mill. essential oil in streptozotocin-induced diabetic rats. Macedonian Journal of Medical Sciences. 2011 Jun 1;4(2):139-46.

60.    Akdogan M, Koyu A, Ciris M, Yildiz K. Anti-hypercholesterolemic activity of Juniperus communis Lynn Oil in rats: A Biochemical and Histopathological Investigation.

61.    Sadrefozalayi S, Farokhi F. Effect of the aqueous extract of Foeniculum vulgare (fennel) on the kidney in experimental PCOS female rats. Avicenna Journal of Phytomedicine. 2014 Mar;4(2):110.

62.    Jiang YH, Jiang LY, Wu S, Jiang WJ, Xie L, Li W, Yang CH. Proteomic analysis reveals the renoprotective effect of Tribulus terrestris against obesity-related glomerulopathy in rats. Biological and Pharmaceutical Bulletin. 2018:b18-00304.

63.    Das RA, Sahu JA, Gupta RN. Potentiative Activity of Cucurbita Maxima Seed Extract With Beta Vulgaris and Smilax Regelii Root Extract To Reduce Extra Fats From The Body. International Journal of Pharmaceutical Sciences and Research. 2010;1:57-62.

64.    Padmapriya R, Gayathri L, Ronsard L, Akbarsha MA, Raveendran R. In vitro anti-proliferative effect of tephrosia purpurea on human hepatocellular carcinoma cells. Pharmacognosy Magazine. 2017 Jan;13(Suppl 1):S16.

65.    Swathi PP, Harindran J. Evaluation Of Hepatoprotective Activity Of Ethanolic Extract Of Adiantum Lunulatum Burm. F. World Journal of Pharmaceutical Research 2018; 7(17): 1198-1207. DOI: 10.20959/wjpr201817-13440.

66.    Gião MS, Gomes S, Madureira AR, Faria A, Pestana D, Calhau C, Pintado ME, Azevedo I, Malcata FX. Effect of in vitro digestion upon the antioxidant capacity of aqueous extracts of Agrimonia eupatoria, Rubus idaeus, Salvia sp. and Satureja montana. Food Chemistry. 2012 Apr 1;131(3):761-7.

67.    Amina RM, Aliero BL, Gumi AM. Phytochemical screening and oil yield of a potential herb, camel grass (Cymbopogon schoenanthus Spreng.). Cent Eur J Exp sCI. 2013;2(3):15-9.

68.    Eddouks M, Lemhadri A, Zeggwagh NA, Michel JB. Potent hypoglycaemic activity of the aqueous extract of Chamaemelum nobile in normal and streptozotocin-induced diabetic rats. Diabetes Research and Clinical Practice. 2005 Mar 1;67(3):189-95.

69.    Kato A, Minoshima Y, Yamamoto J, Adachi I, Watson AA, Nash RJ. Protective effects of dietary chamomile tea on diabetic complications. Journal of Agricultural and Food Chemistry. 2008 Aug 6; 56(17):8206-11.

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