Author(s): Deepak Pradhan, Durga Madhab Kar, Goutam Ghosh

Email(s): goutamghosh@soa.ac.in

DOI: 10.5958/0974-360X.2020.00259.0   

Address: Deepak Pradhan, Durga Madhab Kar, Goutam Ghosh*
School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Kalinga Nagar, Bhubaneswar, Odisha, 751030.
*Corresponding Author

Published In:   Volume - 13,      Issue - 3,     Year - 2020


ABSTRACT:
Natural products isolated from the plant source are considered to be a potent candidate for safe and efficient therapeutic agents since the time immemorial. Numerous bio-potent natural products like Acetylsalicyclic acid, Salicin, Morphine, Digitoxin, Quinine, and Pilocarpine proved to be consecrated in health care sector. Concern to the population exploration, there is inadequate supply of drugs. Last few decades, it is observed that a surge in the herbal drug based dietary supplement in the market. Due to severe unavoidable side effects associated with the allopathic drugs, which led to increase emphasis on isolation of natural products from plant sources. Tinospora cardifolia, belongs to family Menispermaceae has well known for immune booster potency. The preliminary phytochemical screening gives the significance result for presence of alkaloids, terpenoids, glycosides, flavonoids as major phyto-constitution of this plant. Although a lot of phytoconstituents have been isolated from this plant but, still many more phytoconstituents need to be isolated from the polar fractions like n-butanol and water. This research article enlightens the activity targeted isolation of phytoconstituents from the polar fraction of methanolic extract. Different fractions were screened for the antibacterial activity. Considering the zone of inhibition, n-butanolic fraction showed the satisfactory result as compared to other fractions against some selected dental pathogens. So, the n-butanol fraction was considered for phytochemical exploration. The n-butanol fraction festivities a polar compound cordioside and it was characterized with various sophisticated instrumental techniques such as UV-Vis, FTIR, NMR, and Mass Spectroscopy.


Cite this article:
Deepak Pradhan, Durga Madhab Kar, Goutam Ghosh. Bioassay-guided Fractionation of Anti-microbial principle from Ethanolic Extract of Tinospora cordifolia and its Characterization. Research J. Pharm. and Tech 2020; 13(3): 1415-1422. doi: 10.5958/0974-360X.2020.00259.0


REFERENCE:

1.     Sofowora A, Ogunbodede E, Onayade A. The role and place of medicinal plants in the strategies for disease prevention. Afr J Tradit Complement Altern Med. 2013; 10 (5): 210-29.

2.     Hotwani K, Baliga S, Sharma K. Phytodentistry: use of medicinal plants. J Complement Integr Med. 2014; 11 (4): 233-51.

3.     Dias DA, Urban S, Roessner U. A historical overview of natural products in drug discovery. Metabolites. 2012; 2 (2): 303-36.

4.     Veeresham C. Natural products derived from plants as a source of drugs. J Adv Pharm Technol Res. 2012; 3 (4): 200-201.

5.     Rungsung W, Ratha KK, Dutta S, Dixit AK, Hazra J. Secondary metabolites of plants in drugs discovery. World Journal of Pharmaceutical Research. 2015; 4(7): 604-613.

6.     Singh V, Haque S, Singh H, Verma J, Vibha K, Singh R, Jawed A, Tripathi  CK. Isolation, Screening, and Identification of Novel Isolates of Actinomycetes from India for Antimicrobial Applications. Front Microbiol. 2016; 6(7): 1921.

7.     Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga Latha L. Extraction, isolation and characterization of bioactive compounds from plants' extracts. Afr J Tradit Complement Altern Med. 2011; 8(1):1-10.

8.     Martins E. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2013; 4: 177.

9.     Patel N, Patel S, Krishnamurthy R. Indian Tinospora species: natural immunomodulators and therapeutic agents. International Journal of Pharmaceutical Biological and Chemical Sciences. 2013 2(2): 01-09.

10. Sharma R, Galib, Prajapati PK. Remarks on "Tinospora cordifolia: One plant, many roles". Anc Sci Life. 2014; 33(3):194.

11. Prabhakar PK, Lal UR, Suttee A, Kaur G. Phytochemical and Biological Analysis of Tinospora cordifolia. International Journal of Toxicological and Pharmacological Research 2016; 8(4); 297-305.

12. Saha S, Ghosh S. Tinospora cordifolia: One plant, many roles. Anc Sci Life. 2012; 31(4):151-9.

13. Sinha K, Mishra NP, Singh J, Khanuja SPS. Tinospora cordifolia (Guduchi), a reservoir plant for therapeutic applications: A Review. Indian Journal of Traditional Knowledge. 2004; 3(3): 257-270.

14. Pandey MM, Rastogi S, Rawat AKS. Indian Traditional Ayurvedic System of Medicine and Nutritional Supplementation. Evidence-Based Complementary and Alternative Medicine. 2013; 376327:12.

15. Upadhyay AK, Kumar K, Kumar A, Mishra HS. Tinospora cordifolia (Willd.) Hook. f. and Thoms. (Guduchi) - validation of the Ayurvedic pharmacology through experimental and clinical studies. Int J Ayurveda Res. 2010;1(2):112-21.  

16. Patil VS, Malpathak NP. Micro-morphoanatomical approach for comparative analysis of Tinospora cordifolia (Willd.) Miers and its adulterant plant using SEM and Cryostat. Pharmacogn J. 2017; 9(1): 39-4

17. Patil VS, Malpathak NP. Micro-morphoanatomical approach for comparative analysis of Tinospora cordifolia (Willd.) Miers and its adulterant plant using SEM and Cryostat. Pharmacognosy Journal. 2017; 9(1):39-45.

18. Joshi AC. Morphology of Tinospora cordifolia, with Some Observations on the Origin of the Single Integument, Nature of Synergidae, and Affinities of the Menispermaceae. American Journal of Botany. 1939; 26(6): 433-439.  

19. Choudhary N, Siddiqui MB, Khatoon S. Pharmacognostic evaluation of Tinospora cordifolia (Willd.) Miers and identification of biomarkers. Indian Journal of Traditional Knowledge. 2014;13(3): 543-55

20. Saha S, Ghosh S. Tinospora cordifolia: One plant, many roles. Anc Sci Life. 2012; 31(4):151-9.             

21. Sinha K, Mishra NP, Singh J, Khanuja SPS. Tinospora cordifolia (Guduchi), a reservoir plant for therapeutic applications: A Review. Indian Journal of Traditional Knowledge. 2004; 3(3): 257-27.             

22. Singh SS, Pandey SC, Srivastava S, Gupta VS, Patro B, Ghosh AC. And: Chemistry and medicinal properties Indian Journal of Pharmacology. 2003; 35: 83-91.

23. Gupta D, Singh V, Prakash D. Chemical constituents from And stem. Journal of Medicinal and Aromatic Plant Sciences. 2009;31(3): 209-214.

24. Reddy NM, Reddy NR. And Chemical Constituents and Medicinal Properties: A Review. Scholars Academic Journal of Pharmacy. Sch. Acad. J. Pharm. 2015; 4(8): 364-369.

25. Bhan M. And–Phytochemical and Pharmacological evaluation. International Journal of Advanced Scientific Research. 2016; 1 (9): 08-11.  

26. Joshi G, Kaur R. And: A Phytopharmacological Review. Int J Pharm Sci Res. 2016; 7(3): 890-97.

27. Pradhan D, Ojha V, Pandey AK. Phytochemical analysis of And (willd.) Miers ex Hook. F. and Thoms stem of varied thickness. Int J Pharm Sci Res 2013: 4(8); 3051-3056.

28. Mahima, Rahal A, Prakash A, Verma AK, Kumar V, Roy D. Proximate and elemental analyses of And stem. Pak J Biol Sci. 2014; 17(5):744-7.

29. Shanthi V, Nelson R. Anitbacterial activity of And (Willd) Hook. F. Thoms on urinary tract pathogens. Int.J.Curr.Microbiol.App.Sci. 2013; 2(6): 190-194.

30. Jeyachandran R, Xavier TF, Anand SP. Antibacterial activity of stem extracts of And (willd) hook. F and Thomson. Anc Sci Life. 2003; 23(1):40-3.

31. Gul R, Jan SU, Faridullah S, Sherani S, Jahan N. “Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan”. The Scientific World Journal. 2017; Article ID 5873648: 7.

32. Singh P, Katiyar D, Singh B, Srivastava A. Antimicrobial activity of And extracts against urinary tract infections causing bacteria. International Journal of Pharma and Bio Sciences. 2015; 6: B571-B577.

Recomonded Articles:

Author(s): Rakhi Mehra, Renu Makhija, Neera Vyas

DOI: Not Available         Access: Open Access Read More

Author(s): Koushika Das, Pranit Krishna, Avipsha Sarkar, Shanmuga Sundari Ilangovan, Shampa Sen

DOI: 10.5958/0974-360X.2017.00267.0         Access: Open Access Read More

Author(s): U.S Mahadeva Rao, Khamsah Suryati Mohd, Abdurrazaq Muhammad, Bashir Ado Ahmad, Mohaslinda Mohamad, Rosmawati Mat Ali

DOI: Not Available         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Ankit Patel, Pankaj Kushwah, Sujit Pillai, Ajay Raghuvanshi, Nitin Deshmukh

DOI: 10.5958/0974-360X.2017.00013.0         Access: Open Access Read More

Author(s): Pooja Thakur, Chirag Chopra, Daljeet Singh Dhanjal, Reena Singh Chopra

DOI: 10.5958/0974-360X.2017.00466.8         Access: Open Access Read More

Author(s): Josephin Nerling Rashida G., T. Venkatarathnakumar, R. Gowri, Ajithadhas Aruna

DOI: Not Available         Access: Open Access Read More

Author(s): Rupesh Pandey, Priyanka Upadhayay, Shiv Shankar Shukla

DOI:         Access: Open Access Read More

Author(s): Md Kamal Hossain, Kamrun Nahar, Parisa Shokryazdan, Norhani Abdullah, Kaiser Hamid, Mohammed Faseleh Jahromi

DOI: 10.5958/0974-360X.2017.00530.3         Access: Open Access Read More

Author(s): Sandeep DS, Narayana Charyulu R, Prashant Nayak, Aliss Maharjan, Indira Ghalan

DOI: 10.5958/0974-360X.2016.00163.3         Access: Open Access Read More

Author(s): S. Krishnakumar, R. Divya, N.R. Kanchana Devi, G. Keerthana, A. Ancy Judi

DOI: 10.5958/0974-360X.2017.00234.7         Access: Open Access Read More

Author(s): Manisha N. Trivedi, Archana Khemani, Urmila D. Vachhani, Charmi P. Shah, D.D. Santani

DOI: Not Available         Access: Open Access Read More

Author(s): Nadeem Siddiqui, Waquar Ahsan, M. Shamsher Alam, Andalip, Bishmillah Azad, M. Jawaid Akhtar

DOI: Not Available         Access: Open Access Read More

Author(s): T. Sheela Rani, V. Gopal, S. Seethalakshmi, K. Chitra

DOI: Not Available         Access: Open Access Read More

Author(s): Kulkarni Anup, Kale Mohan, Sarode Suraj, Firke Sandip, Firke Bhushan, Warke Prashant

DOI: Not Available         Access: Open Access Read More

Author(s): Zahraa Abdulelah Al Naqqash, Huda Khalil Al-Bazaz, Fatima Mahdi Salh, Shahad Qusay Ibraheem

DOI: 10.5958/0974-360X.2019.00013.1         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags