Author(s): Sasikala M, Sundaraganapathy R, Mohan S

Email(s): sasikalampharm@gmail.com

DOI: 10.5958/0974-360X.2020.00250.4   

Address: Sasikala M1,2, Sundaraganapathy R2, Mohan S1
1Karpagam College of Pharmacy, Coimbatore – 641032, Tamil Nadu, India.
2Faculty of Pharmacy, Karpagam Academy of Higher Education, Coimbatore – 641021, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 3,     Year - 2020


ABSTRACT:
Aim and Objectives: It is to reveal anticancer activity of phytomolecules from the plant using cell line evaluation method and to make the plant as source for treating many chronic diseases like cancer. Method: Hydroalcohlic extract and isolated compounds (E02, E05, W02 and W04) from the selected plant were subjected for anticancer screening using MTT assay method. The cell line used was MCF – 7 for human breast cancer in women. The measurement was carried at a wavelength of 590 nm and the percentage growth inhibition was calculated. The concentrations of test drug needed to inhibit cell growth by 50% (IC50) values were generated from the dose-response curves for each cell line. Results and Discussion: The IC50 values of extract and isolated compounds were detected as 220.9, 276.3, 248.6, 173.1 and 182.8µg/mL respectively. The results showed that isolated compound, W02 has more activity when comparing with other isolates and even extract. All the samples were observed with significant activity. Conclusion: The phytochemicals of the plant with potent anticancer properties could be used to develop herbal formulations for the treatment of breast cancer in women. Hence, the compounds will play major role in society as natural remedy for a life threatening disease.


Cite this article:
Sasikala M, Sundaraganapathy R, Mohan S. MTT Assay on Anticancer Properties of Phytoconstituents from Ipomoea aquatica forsskal using MCF–7 cell lines for breast cancer in Women. Research J. Pharm. and Tech 2020; 13(3):1356-1360. doi: 10.5958/0974-360X.2020.00250.4

Cite(Electronic):
Sasikala M, Sundaraganapathy R, Mohan S. MTT Assay on Anticancer Properties of Phytoconstituents from Ipomoea aquatica forsskal using MCF–7 cell lines for breast cancer in Women. Research J. Pharm. and Tech 2020; 13(3):1356-1360. doi: 10.5958/0974-360X.2020.00250.4   Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-3-55


REFERENCES:

1.     Amrita Mishra, Arun K Mishra and Sanjay K Jain. Anticonvulsant Activity of Cleome viscosa Seed Extracts in Swiss Albino Mice. International Journal of Pharmacy and Pharmaceutical Sciences. 2010, 2(1): 177-181.

2.     Kumar Sarvesh, Maurya Santosh Kumar, Seth Ankit and Singh Anil Kumar.Quality Control Standardization of the Bark of Moringao leifera Lam.International Journal of Pharmacy and Pharmaceutical Sciences. 2015, 7(1): 56-60.

3.     Sasikala M, Mohan S, AjithaVasudevanNambiar, Dona S, Karpakavalli M and Sundaraganapathy R. High Performance Thin Layer Chromatographic Determination of Phytoconstituents in Hydroalcoholic and Methanolic Whole Plant Extract of Peristylusgoodyeroides (D.Don) Lindl. World Journal of Pharmaceutical Research. 2017, 6(10): 1405-1416.

4.     Sasikala M, Mohan S, Haritha T H, Manoj K, Karpakavalli M and Sundaraganapathy R. Extraction And Phytochemical Screening of Whole Plant of PeristylusGoodyeroides (D. Don) Lindl. from Southern Whestern Ghats of India. World Journal of Pharmaceutical Research. 2017, 6(10): 1394-1404.

5.     Sasikala M and Sundaraganapathy R. Optimization of Extraction Method for Ipomoea Aquatica Forssk. [Indian River Spinach] From Its Whole Plant, International Journal of Recent Scientific Research, 2017;8(5): 16967-16971.

6.     Sasikala M, SundaraGanapathy R, Karpakavalli M and Mohan S. Chemotaxonomical and Pharmacological Review on Medicinal Plants in Temperate Region, International Journal of Pharmacy and Technology, 2017, 9(1):29165-29172.

7.     Sasikala M and Sundaraganapathy R. Qualitative Analysis of Alkaloids Exist in the Hydroalcoholic Extract of Ipomoea aquatica Forssk. in Tamil Nadu. International Journal of ChemTech Research, 2017,10(7): 446-454.

8.     Sasikala M and Sundaraganapathy R. Fluorescence Detection of Phytoconstituents in Hydroalcoholic Extract of Ipomoea aquatica Forssk. by Thin Layer Chromatographic Analysis, International Journal of Pharmacognosy and Phytochemical Research, 2017; 9(7): 990-996.

9.     Sasikala M and Sundaraganapathy R. Preliminary Phytochemical Evaluation of Hydroalcoholic Extract of Ipomoea Aquatica Forssk.from Aliyar Riverinein South India, Int J Pharma Bio Sci, 2017; 8(3): 356-365.

10. Sasikala M, Karpakavalli M, Ranjithkumar D, Prakash G, Vanathi P and Mohan S. In-vitro Free Radical Scavenging Activity Studies of Extracts and Isolated Compounds of Eugenia jambolana Lam. Seeds, World Journal of Pharmaceutical Research, (SJIF Impact Factor 6.805), 2016, Volume 5, Issue 6, 2021-2030.

11. Kishore Konam, Jilla Soujanya, Sasikala M and Kiran kumar A. Development and validation of RP-HPLC method for the determination of Bisoprolol fumarate tablets, International Journal of Research in Pharmaceutical and Nano Sciences. 2(1), 2013, 57-67.

12. Sasikala M, Nithya S, Gowthami C, Bojjaaswini, and Jamuna KM. Application of UV Spectrophotometric Method for Drug Interaction Studies of Mefenamic Acid with Ofloxacin, International Journal of Research in Pharmaceutical and Nano Sciences. 1(2), 2012, 274- 280.

13. Sasikala M, Bojjaaswini, Gowthami CL, Nithya S, Jamuna KM and Kiran kumar A. Extraction, Isolation, Characterization of Phytoconstituents from Curcuma Caesia Roxb by Various Analytical Methods, International Journal of Research in Pharmaceutical and Nano Sciences. 1(2), 2012, 257- 268.

14. A.H. Beckett and J.B. Stenlake. Practical pharmaceutical chemistry. Thin Layer Chromatography. CBS Publishers. 4th edition. 2005:115-128.

15. S.S. Khadabadi, S.L. Deore and B. A. Baviskar. Experimental Phytopharmacognosy– A Comprehensive guide. Nirali Prakashan, Pune, Maharashtra. Second edition, 2013:p.3.1-3.13, A 1.1-2.8.

16. Harborne JB. Phytochemical Methods. Chapman and hall Ltd., London: U.K., 1973, 49-188.

17. GuntiGowtham RajHyma Sara Varghese, Sarita Kotagiri, B. M. Vrushabendra Swamy, Archana Swamyand Rafi Khan Pathan. Anticancer Studies of Aqueous Extract of Roots and Leaves of Pandanus Odoratissimus f. ferreus (Y. Kimura) Hatus: An In Vitro Approach. J Tradit Complement Med. 2014 Oct-Dec; 4(4): 279–284.

18. Sumitra Chanda and Krunal Nagani. In vitro and in vivo Methods for Anticancer Activity Evaluation and Some Indian Medicinal Plants Possessing Anticancer Properties: An Overview. Journal of Pharmacognosy and Phytochemistry. 2013; 2(2):140-152.

19. P.A. Shah, J.M. Parekh, P.S. Shrivastav Assessment of critical extraction and chromatographic parameters for the determination of bupropion and its three primary metabolites in human plasma by LC-MS/MS. Microchem. J., 135 (2017), pp. 81-90.

20. Kumaran S, NazeemaBanu B, Julie J, Abirami J, Kumareasan R, Muthukumaran T, Rajasree S and JeyaJothi K.Anti-cancer activity of Daturametel in MCF-7 cell line. Asian Journal of Pharmaceutical and Clinical Research. 2014;  7 (1): 181-183.

21. Mukundam Borah, Swarnamoni Das and Shagufa Ahmed. Antibacterial activity of the ethanolic extract of leaves of Citrus maxima (Burm.) Merr. on Escherichia coli and Pseudomonas aeruginosa. Asian Journal of Pharmaceutical and Clinical Research. 2013; 6(4): 136-139.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available