Author(s):
Harshita H. Patel, Vishwas C. Bhagat, Rajkumar V. Shete, Amit S. Ravetkar
Email(s):
patelharshita558@gmail.com , rvshete09@gmail.com
DOI:
10.5958/0974-360X.2020.00242.5 
Address:
Harshita H. Patel1*, Vishwas C. Bhagat1, Rajkumar V. Shete2, Amit S. Ravetkar3
1Department of Quality Assurance and Techniques, Rajgad Dnyanpeeth’s College of Pharmacy Bhor-Pune, Maharashtra-India.
2Department of Pharmacology Rajgad Dnyanpeeth’s College of Pharmacy Bhor-Pune, Maharashtra-India.
3Piramal Enterprises LTD, Mahad Raigad, Maharashtra India.
*Corresponding Author
Published In:
Volume - 13,
Issue - 3,
Year - 2020
ABSTRACT:
To Develop and Validate Analytical method which is simple, rapid and sensitive for the determination of biotin from premixes (solid blend of multi-vitamin) by RP-HPLC. RP-HPLC analysis was performed using HPLC Agilent Technology 1260 Infinity series HPLC. The chromatographic separation was obtained by using column C18 Thermo scientific ODS Hypersil,150×4.6,3 µ,mobile phase used was buffer: acetonitrile in the ratio 91.5:8.5, Flow rate 1.2 mi/min, wavelength 200nm, Injection volume 100 micro lit, column oven temp. 40°C, run time: 15 min. The retention time of biotin was found to between 10-12 min. Calibration curve was linear with a correlation coefficient of 0.996 over the concentration range of 50-150 ppm and precise with % RSD < 2 for standard and for sample % RSD < 5. The method was found to be specific, linear, precise and accurate. Suitable for the Quality control analysis.
Cite this article:
Harshita H. Patel, Vishwas C. Bhagat, Rajkumar V. Shete, Amit S. Ravetkar. Analytical Method Development and Validation for biotin from Premixes (solid blend of Multi-Vitamin) by RP-HPLC. Research J. Pharm. and Tech 2020; 13(3):1314-1318. doi: 10.5958/0974-360X.2020.00242.5
Cite(Electronic):
Harshita H. Patel, Vishwas C. Bhagat, Rajkumar V. Shete, Amit S. Ravetkar. Analytical Method Development and Validation for biotin from Premixes (solid blend of Multi-Vitamin) by RP-HPLC. Research J. Pharm. and Tech 2020; 13(3):1314-1318. doi: 10.5958/0974-360X.2020.00242.5 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-3-47
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