Author(s): Febri Annuryanti, Asri Darmawati, Andang Miatmoko, Kustiawan


DOI: 10.5958/0974-360X.2020.00238.3   

Address: Febri Annuryanti, Asri Darmawati, Andang Miatmoko, Kustiawan
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya 60286, Indonesia.
*Corresponding Author

Published In:   Volume - 13,      Issue - 3,     Year - 2020

Development and validation of UV-Vis Spectrophotometry method for simultaneous determination of primaquine and chloroquine in liposome dosage form has been carried out. The method was tested for selectivity, linearity, accuracy, and precision. A phosphate buffer solution pH 7.4 was used as a solvent and observations were made at wavelengths of 220 and 260 nm for simultaneous equations method. The selectivity results of primaquine and chloroquine showed no interference from liposome. Linearity result (r value) of the simultaneous equation method was 0.9998 with the value of Vxo less than 0.5% for both primaquine and chloroquine in the concentration range of 2-10 mg/L. Accuracy was done using spiked placebo method and obtained data analyzed using simultaneous equation method. Persentage recovery of primaquine was 89-97% and 79 - 108% for chloroquine. The intra- and interday precision of primaquine were 1.72 and 2.57%, respectively. Whereas the intra- and interday precision of chloroquine were 6.93 and 8.77, respectively. Further observation using chromatography method need to be done to have better accuracy results for both substances.

Cite this article:
Febri Annuryanti, Asri Darmawati, Andang Miatmoko, Kustiawan. Development and Validation of Spectrophotometry UV-Vis Method for Determination of Primaquine and Chloroquine in Liposome Dosage Form. Research J. Pharm. and Tech 2020; 13(3): 1293-1296. doi: 10.5958/0974-360X.2020.00238.3


1.      World Health Organization. Guidelines for the Treatment of Malaria.WHO Press, Switzerland, 2015.

2.      Ministry of Health of the Republic of Indonesia. Malaria. Indonesian Ministry of Health Data and Information Center, Jakarta, 2016.

3.      Mahalakshmi Thillainayagam and Sudha Ramaiah. Mosquito, Malaria and Medicines – A Review. Research J. Pharm. and Tech 2016; 9(8):1268-1276.

4.      National Center for Biotechnology Information. PubChem Compound Database; CID=4908, Available from : URL:

5.      Asia Pasific Malaria Elimination Network (APMEN). The Asia Pacific Elimination Network is Dedicated To Supporting Malaria Elimination in Asia and The Pacific by 2030. APMEN, 2017.

6.      American Hospital Formulary Service. Primaquine. In AHFS Drug Information, Edited by McEvoy, G.K.. American Society of Health-System Pharmacists, Bethesda, MD. Wisconsin, USA. 2006. p. 869

7.      Rhea J. Longley et al. Insight into Naturally Acquired Immune Response to Plasmodium Vivax Malaria. Parasitology 2016; 143 (2): 154-170.

8.      Cevc, G and Richardsen, H. Lipid vesicles and membrane fusion. Advanced Drug Delivery Reviews 1993; 38 (3): 207–232. 

9.      Chu, C.S. and White, N.J. Management of Replacing Plasmodium Vivax Malaria, Expert review of anti-infective therapy 2016; 14(10): 85-900.

10.   Barani, H and Montazer, M. A review on applications of liposomes in textile processing. Journal of liposome research 2008; 18 (3): 249–62. 

11.   Barenholz, Y and G, Cevc. Structure and properties of membranes. In Physical chemistry of biological surfaces. Marcel Dekker, New York. 2000: 171–241.

12.   Bertrand, Nicolas et al. Transmembrane pH-Gradient Liposomes to Treat Cardiovascular Drug Intoxication. ACS Nano 2010; 4 (12): 7552–8. 

13.   Yenny Meliana. Obat Baru Malaria Diteliti. Kompas, Jakarta, 30 November 2016.

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