Author(s): Niran A. Al-Ogaili, Safa Osama, Doha Jazme, Sara Saad

Email(s): ayjasem@yahoo.com

DOI: 10.5958/0974-360X.2020.00221.8   

Address: Niran A. Al-Ogaili*, Safa Osama, Doha Jazme, Sara Saad
Pharmacy Department, Al-Yarmouk University College, Baghdad, Iraq.
*Corresponding Author

Published In:   Volume - 13,      Issue - 3,     Year - 2020


ABSTRACT:
Plants are potential origin of antimicrobial drugs in different countries. They contain a broad range of phytochemicals that possess antimicrobial action against various mirco-organisms. The popularity of diseases caused by bacteria world-wide is a great public health issue and the emergence of resisting strains of bacteria to anti-bacterials lead to many studies looking for new anti-bacterials to fight the diseases and resistance to antibiotic. In this study, aqueous leaf extract of olive (Olea europea) and fig (Ficus carica) were investigated for their antibacterial activities against three bacterial strains Staphylococcus aureus, Escherichia coli and Pseudomonos aeruginosa. Muller-Hinton agar and well diffusion method used for assessment of inhibitory activity. The phytochemical identification of some active compounds performed by chemical methods. The results were positive for all the tested phytochemicals (alkaloids, tannins, saponin, glycosides, flavonoids, phenolic compounds and coumarine) for figs and olive aqueous extracts, while negative result for oleurupein in figs extract. Both extracts exhibited strong activity against the bacterial strains. Significant results shown for the combination of both water concentrates of figs and olives. The use of these extracts together is highly recommended to induce synergism and beneficial in developing novel antibiotics from natural, inexpensive sources to overcome bacterial resistance.


Cite this article:
Niran A. Al-Ogaili, Safa Osama, Doha Jazme, Sara Saad. In Vitro Antibacterial investigation and Synergistic Effect of Ficus carica and Olea europaea Aqueous Extracts. Research J. Pharm. and Tech 2020; 13(3):1198-1203. doi: 10.5958/0974-360X.2020.00221.8

Cite(Electronic):
Niran A. Al-Ogaili, Safa Osama, Doha Jazme, Sara Saad. In Vitro Antibacterial investigation and Synergistic Effect of Ficus carica and Olea europaea Aqueous Extracts. Research J. Pharm. and Tech 2020; 13(3):1198-1203. doi: 10.5958/0974-360X.2020.00221.8   Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-3-26


REFERENCES:

1.      Alviano DS, Alviano CS . Plant extracts: search for new alternatives to treat microbial diseases. Curr Pharm Biotechnol  (2009) 10, 106–121.

2.      Malini M, Abirami G, Hemalatha V, Annadurai G . Antimicrobial activity of ethanolic and aqueous extracts of medicinal plants against waste water pathogens. Inte J Res Pure Appl Microbiol  (2013) 3, 40–42.

3.      Zhang R, Eggleston K, Rotimi V, Zeckhauser RJ.  Antibiotic resistance as a global threat: evidence from China, Kuwait and the United States. (2006) Global Health 2, 6.

4.      Dorman HJ, Deans SG.  Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol  (2000) 88, 308–316.

5.      Talib WH, Mahasneh AM. Antimicrobial, cytotoxicity and phytochemical screening of Jordanian plants used in traditional medicine. Molecules  (2010) 15, 1811–1824.

6.      Cruz MC, Santos PO, Barbosa AM Jr., de Melo DL, Alviano CS, Antoniolli AR, et.al. Antifungal activity of Brazilian medicinal plants involved in popular treatment of mycoses. J Ethnopharmacol  (2007) 111, 409–412.

7.      Ruberto G, Baratta MT, Deans SG, Dorman HJ. Antioxidant and antimicrobial activity of Foeniculum vulgare and Crithmum maritimum essential oils. Planta Med  (2000) 66, 687– 693.

8.      Williamson DA, Heffernan H, Sidjabat H, Roberts SA, Paterson DL, Smith M,et.al.. Impact of antibiotic resistance in gram-negative bacilli on empirical and definitive antibiotic therapy. Clin Infect Dis  (2008) 1, 14–20.

9.      Cheesbrough M (1984). Microscopic examination of specimens. In: Medical Laboratory Manual for Tropical Countries, Oxford, Publishers, United Kingdom,  (1984) pp. 32–33

10.   Peirano G. Multi resistant enterobacteriaceae new threat to an old prob; expect review of anti- infective therapy. Expert Rev Anti Infect Ther  (2008) 6, 657–669.

11.   Eggleston K, Zhang R, Zeckhauser RJ. The global challenge of antimicrobial resistance: insights from economic analysis. Int J Environ Res Public Health  (2010) 7, 3141–3149.

12.   Jander, EA. and Machado, KC. Evolutionary ecology of figs and their associates: Recent progress and outstanding puzzles. Ann Rev Evol. Syst.  (2008)  39:439-458.

13.   J. A. Vinson, L. Zubik, P. Bose, N. Samman, and J. Proch . “Dried fruits: excellent in vitro and in vivoantioxidants,” Journal of the American College of Nutrition, (2005) vol. 24, no. 1, pp. 44–50. 

14.   Vinson J.A. “The functional food properties of figs,” Cereal Foods World, (1999) vol. 44, no. 2, pp. 82–87.

15.   J. A. Vinson, Y. Hao, X. Su, and L. Zubik. “Phenol antioxidant quantity and quality in foods: vegetables,” Journal of Agricultural and Food Chemistry, (1998)  vol. 46, no. 9, pp. 3630–3634. 

16.   J. A. Duke, M. J. Bugenschutz-godwin, J. Du collier, and P. K. Duke. Hand Book of Medicinal Herbs, CRC Press, Boca Raton, Fla, USA, 2nd edition (2002).

17.   M. Werbach. Healing with Food, Harper Collins, New York, NY, USA (1993).

18.   Samova LI, Shode FO, Ramnanan P, Nadar A. Antihypertensive, antiatherosclerotic and antioxidantactivity of triterpenoids isolated from Olea europaea, subspecies Africana leaves. J Ethnopharmacol; (2003) 84: 299- 305.

19.   Khan MY, Panchal S, Vyas N, Butani A, Kumar V. Olea europaea: A Phyto-Pharmacological Review. Pharmacog Rev; (2007) 1(1): 112-6.

20.   Ribeiro RDL, Melo FD, Barros FD, Gomes C, Trolin G. Acute antihypertensive effect in conscious rats produced by some medicinal plants used in the state of Sao Paolo, J Ethnopharmacol; (1986) 15(3): 261-9.

21.   Zarzuelo A, Duarte J, Jimenez J, Gonzalez M, Utrilla MP.  Vasodilator effect of olive leaf. Planta Med; (1991)  57: 417-419.

22.   Bisignano G, Tomaino A, Cascio RL, Crisafi G, Uccella N, Saija A.  On the in-vitro antimicrobial activity of oleuropein and hydroxytyrosol. J Pharm Pharmacol; (1999) 51: 971-4.

23.   Visioli F, Bellomo G, Galli C.  Oleuropein protects low density lipoprotein from oxidation. Life Sci; (1994) 55(24): 1965-71.

24.   Al-Azzawie HF, Alhamdani MS.  Hypoglycemic and antioxidant effect of oleuropein in alloxan-diabetic rabbits. Life Sci; (2006) 78: 1371-7.

25.   Hardalo, C. & Edberg, S. C.  Pseudomonas aeruginosa: assessment of risk from drinking water. Crit. Rev. Microbiol. (1997) 23, 47–75.

26.   Costerton, J. W., Stewart, P. S. & Greenberg, E.P. Bacterial biofilms: a common cause of persistent infections. Science  (1999) 284, 1318– 1322.

27.   Ahearn, D. G., Borazjani, R. N., Simmons, R. B. & Gabriel, M. M. Primary adhesion of Pseudomonas aeruginosa to inanimate surfaces including biomaterials. Methods Enzymol. (1999)  310, 551– 557.

28.   Bodey, G. P., Bolivar, R., Fainstein, V. & Jadeja, L. Infections caused by Pseudomonas aeruginosa. Rev. Infect. Dis.  (1983) 5, 279–313.

29.   Tenaillon, Olivier; Skurnik, David; Picard, Bertrand; Denamur, Erick . "The population genetics of commensal Escherichia coli". Nature Reviews Microbiology.  (2010) 8 (3): 207–217.

30.   Singleton P. Bacteria in Biology, Biotechnology and Medicine (5th ed.). Wiley. (1999)  pp. 444–454.

31.   Kaper JB, Nataro JP, Mobley HL . Pathogenic Escherichia coli. Nat Rev Microbiol.2(2): (2004) 123-140.

32.   Lowy FD. Antimicrobial resistance: the example of Staphylococcus aureus. J Clin Invest.; (2003) 111: 1265–1273.

33.   McCaig LF, McDonald LC, Mandal S, Jernigan DB. Staphylococcus aureus-associated skin and soft tissue infections in ambulatory care. Emerg Infect Dis.; (2006) 12:1715–1723.

34.   Gorwitz RJ, et al.   Changes in the prevalence of nasal colonization with Staphylococcus aureus in the United States,  2001–2004. J Infect Dis.; (2008) 197:1226–1234.

35.   Hidron AI, et a .  Risk factors for colonization with methicillin-resistant Staphylococcus aureus (MRSA) in patients admitted to an urban hospital: emergence of community-associated MRSA nasal carriage. Clin Infect Dis.; (2005) 41:159–166.

36.   Harbone J.B  “Herbivores: The interaction with secondary plants metabolites”. John Wiley and sonc, Inc, New York,  (1979) vol. 1, pp.619.

37.   Obasi NL, Egbuonu Acc, Ukoha PO, Ejikeme PM.  Comparitive phytochemical and antimicrobial screening of some solvent extracts of Samanea sanan pods. African Journal of pure and applied chemistry. (2010) 4(9): 206-212.

38.   Andu SA, Mohammed I, Kaita HA . Phytochemical screening of leaves of  Lophira lanceolata (Chanaceae). Life science Journal. (2007) 4(4): 75-79.

39.   Trease GE, Evans Wc  Pharmacognosy (15th Edn. Saunders,  (2002)   pp.  214-393).

40.   Murry PR, Baron EJ, Pfaller MA, Tenover FC, Yolken HR  Manual of clinical Microbiology, 6th Ed. ASM Press, Washington DC. (1995)  pp. 15-18.

41.   NCCLS, (2003). Performance Standards for Antimicrobial Disk Susceptibility Test, 8th edition M2-M8, National Committee for Clinical Laboratory Methods, Wayne, PA

42.   SAS (2012). Statistical Analysis System, User’s Guide. Statistical Version 9. 1st ed. SAS. Inst. Inc. Cary. N.C. USA.

43.   Parekh, J., Nair, R., Chanda, S. Preliminary screening of some folkloric plants from Western India for potential antimicrobial activity. Indian J. Pharmacol, (2005)  37:408-409.

44.   Aliabadi, M., Darsanaki, R. Rokhi, M., Nourbakhsh M., Raeisi, G. Antimicrobial activity of olive leaf aqueous extract. Annals of Biological Research, (2012) 3(8): 4189-4191.

45.   Al-Askari, G. Kahouadji, A. Khedid, K. Ouaffak, L. Mousaddak, M. Charof, R. Mennane, Z. In vitro antimicrobial activity of aqueous and ethanolic extracts of leaves of Ficus carica collected from five different regions of Morocco. J. Mater. Environ. Sci. (2013) 4 (1):33-38.

46.   Kamak, R., Ikramullah, Jadoon S., Bahadar N., Sajid, K., Fida W., Anwarullah, Khan, S., Akhatar N.,Jadoon A., Alvi I., Rehman M. Antibacterial Activity of aqueous and ethanolic leaves extracts of Ficus carica. Online Journal of Bioscience and Informatics; (2014) Vol: 1, Issue 2.

47.   Markin, D. Duek, L., Berdicevsky I. (2003). Mycoses; 46: 132-136.

48.   Alhamed A. Improvement extraction of crude compounds from Iraqi olive leaves by applying water base and alcoholic- base extraction and their biological applications. World Journal of Pharmacy and Pharmaceutical Sciences; (2015) Vol. 4, Issue 1: 183-200.

49.   Pereira AP., Ferreira I., Marcelino F., Valentao P. Andrade PB., Seabra L., Estevinho L., Bento A., Pereira JA.(2007). Molecules; 12, 1153-1162.

50.   Breitinger HG. Drug Synergy: Mechanisms and methods of analysis. Chapter 7 ”Toxicity and Drug Testing”. The German University in Cairo, Egypt / Faculty of Pharmacy and Biotechnology(2012).

51.   I. Vagelas, H. Kalorizou, A. Papachatzis, and M. Botu . ”Bioactivity of olive oil mill wastewater against plant pathogens and post harvest diseases”. Biotechnology and Biotechnological Equipment; (2009)vol.23, no.2,p. 1219.

52.   C. Valenta, E. Schwarz, and A. Bernkop- Schnurch . “Lysozyme-caffeic acid conjugates: possible novel preservatives for dermal formulations” International Journal of Pharmaceutics: (1998) vol. 174, no. 1-2, pp. 125-132.

53.   A. Sousa, I.C.F.R., Ferreira, R. Calhella et. al. “Phenolics and antimicrobial activity of traditional stoned table olives alcaparra” Bioorganic and Medicinal Chemistry; (2006) vol.14, no. 24, pp. 8533-8538.

54.   R.H., Rogez, D. Campus, R. Pedreschi, and Y. Larondelle “Optimization of extraction conditions of antioxidant phenolic compounds from Malua(Tropaeolum tuberosum Ruiz and Paron) tubers. Sep Purif Technol. (2007),55: 217-225.

55.   The Holy Qur’an(Chapter Al-Tin 1-6).

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