Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo

Sanjukta Badhai; Durga Barik; Bairagi C. Mallick

doi:10.5958/0974-360X.2020.00211.5

Research Journal of Pharmacy and Technology

ISSN

0974-360X (Online)
0974-3618 (Print)

Submit Article

Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo

Author(s): Sanjukta Badhai, Durga Barik, Bairagi C. Mallick

Email(s): bcmallick@ravenshawuniversity.ac.in

DOI: 10.5958/0974-360X.2020.00211.5

Address: Sanjukta Badhai1, Durga Barik1, Bairagi C. Mallick2
1Department of Botany, Ravenshaw University, Cuttack-753003, Odisha, India.
2Department of Chemistry, Ravenshaw University, Cuttack-753003, Odisha, India.
*Corresponding Author

Published In: Volume - 13, Issue - 3, Year - 2020

View HTML

View PDF

ABSTRACT:
Healthcare is a challenging subject for medical science. Drug delivery system is a knowing technique to prevent and cure too many diseases. Here in this study is to examine ?-Sitosterol loaded Hydroxyapatite-Alginate Nanocomposite using drug delivery systems in vivo. The anticancer activities of ?-Sitosterol loaded Hydroxyapatite-Alginate are studied against Dimethyl hydrazine (DMH) induced colon cancer. Colon cancer was induced in male sprague-dawley rats by subcutaneous injection for 15 weeks with DMH (20 mg/kg). After complete of the experiment, the blood and colonic tissue sample were collected from rat. Then serum was separated from blood sample for biochemical and haematological analysis and colonic tissue was used for histopathology and antioxidant analysis. In result, it was identified that high lipid peroxidation and reduced antioxidants stage such as SOD, CAT, GST, GPX and GSH. The intoxication DMH altered haematological parameters measurement such as Hb, WBC and RBC. Further, it increased lipid profiles, tumour markers (AFP and CEA) and changed colonic tissue histology. In conclusion we observed that treatment with ?-Sitosterol loaded Hydroxyapatite-Alginate nanocomposite has considerably restored and changed biochemical parameters in DMH induced colon cancer. This clearly signifies the efficacy of ?-Sitosterol as an anticancer agent.

Keywords:

Cite this article:
Sanjukta Badhai, Durga Barik, Bairagi C. Mallick. Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo. Research J. Pharm. and Tech 2020; 13(3): 1147-1151. doi: 10.5958/0974-360X.2020.00211.5

Cite(Electronic):
Sanjukta Badhai, Durga Barik, Bairagi C. Mallick. Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo. Research J. Pharm. and Tech 2020; 13(3): 1147-1151. doi: 10.5958/0974-360X.2020.00211.5 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-3-16

REFERENCES:

1. Guicheux, J. Grimandi, G., Trecant, M. Faivre, A. Takahashi, S. and Daculsi, G. Apatite as carrier for growth hormone: In-vitro characterization of loading and release. J. Biomed Mater Res. 1997, 34, 165

2. Guicheux, J. Grimandi, G., Trecant, M. Faivre, A. Takahashi, S. and Daculsi, G. Apatite as carrier for growth hormone: In-vitro characterization of loading and release. J. Biomed Mater Res. 1997, 34, 165

3. Bajpai, P. K. and Benghuzzi, H.A. Ceramic systems for long-term delivery of chemicals and biologicals. J. Biomed Mater Res. 1988, 22, 1245

4. Ferra, M. P. Monteiro, F. J. and Manuel, C. M. Hydroxyapatite nanoparticles: A review of preparation Methodologies. Journal of Applied Biomaterials and Biomechanics. 2004), 2, 74

5. Tavano, L. Aiello, R. Ioele, G. Picci, N. Muzzalupo, R. Nanocomposites from glucuronic acid-based surfactant as new carriers for cancer therapy: preparation, characterization and biological properties. Colloids Surf B Biointerfaces.2014, 118: 7–13.

6. Lewis, S.M. Bain, B. J. Bates, I. Dacie, J. V. and Lewis. Practical haematology. Philadelphia: Churchill Livingstone/Elsevier. 2006

7. Reitman, S. Frankel, S. A. Colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol. 1957, 28: 56-63.

8. Plummer D.T. An introduction to Practical Biochemistry. 3rdedition. McGraw – Hill Book Company, Maidenhead, Berkshire. 1978, pp. 347.

9. Gagandeep, C. Sandeep, G. Priyanka, P. Int J Pharm Sci Drug Res. 2010, 2: 91.

10. Lowry, O. H. Rosebrough, N.J. Farr, A. L. Randall, R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951, 193: 265-75.

11. Folch, J. Lees, M. Stanley, G.H.S. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957, 226: 497-509.

12. Foster L.B. Dunn R.T. Stable reagents for determination of serum triglycerides by colorimetric Hantzsch condensation method. Clin Chem. 1973, 19: 338-40.

13. Zlatkis, A. Zak, B. Boyle A.J. A new method for the direct determination of serum cholesterol. J Lab Clin Med. 1953, 41:486-92.

14. Misra, H.P, Fridovich, I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972, 247: 3170-3175.

15. Sinha, A.K. Colorimetric assay of catalase. Anal Biochem. 1972, 47: 389-94.

16. Rotruck, J.T. Pope, A.L. Ganther, H.E. Swanson, A.B, Hafeman, D.G. Hoekstra, W.G. Selenium. Biochemical role as a component of glutathione peroxidase. Science. 1973, 179: 588-90.

17. Habig, W.H. Pabst, M.J. Jakoby, W.B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974, 249: 7130-9.

18. Ellman, G.L. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959, 82: 70-77

19. Ohkawa, H. Ohishi, N. Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979, 95: 351-8.

20. Jia, X.D. Han, C. Chemoprevention of tea on colorectal cancer induced by dimethylhydrazine in Wistar rats. World J Gastroenterol. 2000, 6: 699–703.

21. Manju, V. Nalini, N. Chemopreventive efficacy of ginger, a naturally occurring anticarcinogen during the initiation, post-initiation stages of 1,2 dimethylhydrazine-induced colon cancer. Clin Chim Acta. 2005, 358: 60-67.

22. Bains, J.S. Shaw, C.A. Neurodegenerative disorders in humans: the role of glutathione in oxidative stress-mediated neuronal death. Brain Res Brain Res Rev. 1997, 25:335-58.

23. Sivagami, G. Karthikkumar, V. Balasubramanian, T. Nalini, N. The modulatory influence of p-methoxycinnamic acid, an active rice bran phenolic acid, against 1,2-dimethylhydrazine-induced lipid peroxidation, antioxidant status and aberrant crypt foci in rat colon carcinogenesis. Chem Biol Interact. 2012, 196 :11-22.

24. Reddy, B.S. Mangat, S. Scheinfil, A. Weisburger, J.H. Wynder, E.L. Effect of type and amount of dietary fat and 1,2-dimethylhydrazine on biliary bile acids, fecal bile acids, and neutral sterols in rats. Cancer Res. 1977, 37: 2132-7.

25. Chithra, V. Leelamma, S. Coriandrum sativum- effect on lipid metabolism in 1,2-dimethyl hydrazine induced colon cancer. J Ethnopharmacol. 2000, 71: 457–63.

26. Sengottuvelan, M. Nalini, N. Resveratrol, A Phytoalexin Enhances Hepatic Antioxidant Defense in 1, 2-dimethylhydrazine-induced Colon Carcinogenesis. Int J Pharmacol. 2006, 2:335-40.

27. Ogata, Y. Murakami, H. Sasatomi, T. Ishibashi, N. Mori, S. Ushijima, M. Elevated preoperative serum carcinoembrionic antigen level may be an effective indicator for needing adjuvant chemotherapy after potentially curative resection of stage II colon cancer. J Surg Oncol. 2009, 99: 65–70.

28. Yachida, S. Fukushima, N. Nakanishi, Y. Akasu, T. Kitamura, H. Sakamoto, M. et al. Alpha-fetoprotein-producing carcinoma of the colon: report of a case and review of the literature. Dis Colon Rectum. 2003, 46: 826–31.