Author(s): Uttam Singh Baghel, Himani Sharma, Anamika Chouhan, Abhay Sharma, Mohammad Mukim, Deeksha Singh

Email(s): drusb1985@yahoo.com , dixa250786@yahoo.in

DOI: 10.5958/0974-360X.2020.00110.9   

Address: Uttam Singh Baghel1*, Himani Sharma2, Anamika Chouhan3, Abhay Sharma3, Mohammad Mukim3, Deeksha Singh4*
1Department of Pharmacy, University of Kota, MBS Marg, Swami Vivekanand Nagar, Kota 324005, Rajasthan, India.
2Department of Pharmaceutical Analysis, ASBASJSM College of Pharmacy, BELA (Ropar) Punjab-140111, India.
3Kota College of Pharmacy, SP-1, Industrial Area, Jhalawar Road, Ranpur, Kota, 325003, Rajasthan, India.
4ESI Hospital, Jhalawar Road, Vigyan Nagar, Kota, Rajasthan, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 2,     Year - 2020


ABSTRACT:
A novel, sensitive, and specific gradient RP-HPLC method with UV detection is proposed for the simultaneous quantification of multicomponent liquid dosage form. The separation of the three drugs was achieved using a C18 column (250 mm×4.0 mm, 5 µ) as a stationary phase. No preliminary extraction procedure is required for liquid formulations and a very simple extraction procedure is required for tablets and creams. A mixture of two mobile phases was used with gradient time programing at a flow rate of 1 ml/min. Detection was performed by using PDA detector at 270 nm. Phenylephrine HCl (RT=7.705 min), triprolidine HCl (RT=26.510 min) and dextromethorphan HBr (RT=29.166 min) were separated with good resolution in a single chromatographic run of 50 min. Each drug obeys Beer-Lambert’s law in the linearity range of 0.080-0.12 mg/ml for dextromethorphan HBr with a correlation coefficient of 0.998, 0.040–0.060 mg/ml for phenylephrine HCl with a correlation coefficient of 0.996, and 0.010–0.015 mg/ml for triprolidine HCl with a correlation coefficient of 0.998. The recoveries for dextromethorphan HBr and phenylephrine HCl were found to be 99.35% and 99.53%, respectively and for triprolidine HCl was 98.88%. Validation results show satisfactory linearity, precision, accuracy and specificity.


Cite this article:
Uttam Singh Baghel, Himani Sharma, Anamika Chouhan, Abhay Sharma, Mohammad Mukim, Deeksha Singh. Gradient RP-HPLC Method development for simultaneous estimation of Dextromethorphan hydrobromide, Phenylephrine hydrochloride, and Triprolidine hydrochloride in Liquid Dosage Form. doi: 10.5958/0974-360X.2020.00110.9


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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