Author(s): Anshuman Rai, Anamika Gautam, Sakshi Panchal, Ankita Sood, Pankaj Prashar, Narendra Kumar Pandey, Indu Melkani, Bimlesh Kumar

Email(s): bimlesh1Pharm@gmail.com , bimlesh.12474@lpu.co.in

DOI: 10.5958/0974-360X.2020.01070.7   

Address: Anshuman Rai, Anamika Gautam, Sakshi Panchal, Ankita Sood, Pankaj Prashar, Narendra Kumar Pandey, Indu Melkani, Bimlesh Kumar*
School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 12,     Year - 2020


ABSTRACT:
The plant Terminalia arjuna is claimed to be useful in the management of renal disorders. Recurrence of stone formation is the major concern worldwide due to partial and unsatisfactory relief. In the present study, the preventive and curative activity against kidney stones in urolithiatic rats was evaluated using bark of Terminalia arjuna. Preliminary phytochemical investigation using various test, estimation of phenolic and flavonoid content followed by antioxidant potential was also investigated. Invitro antiurolithiatic effect on artificial urine and in vivo effect in terms of curative and preventive potential on animals were reported. Urolithiasis was developed in rats by administration of ethylene glycol 0.75% v/v with 1% w/v ammonium chloride in drinking water. The ethanolic extract of T. arjuna at 200 and 400 mg/kg were administered to rat orally. Urine was collected from individual rats and used for investigation of various parameters like urine calcium, phosphate, oxalate and BUN. In serum, creatinine, calcium and uric acid levels were also estimated. The kidney homogenate was used for the estimation of oxidative biomarkers and histopathological reports. Terminalia arjuna bark extract showed the presence of tannin, terpenoids, saponin, flavonoids, glycosides, phenolic compounds, alkaloids etc and showed good amount of phenolic and flavonoid contents. The lithogenic induction caused impairment of renal function and oxidative stress, manifested as increased malondialdehyde, depleted reduced glutathione and decreased catalase activities of the kidneys, which were reversed by T. arjuna orally at both the doses in preventive as well as curative model. Thus, the current study revealed that the bark of Terminalia arjuna was endowed with antiurolithiatic activity possibly through an antioxidant.


Cite this article:
Anshuman Rai, Anamika Gautam, Sakshi Panchal, Ankita Sood, Pankaj Prashar, Narendra Kumar Pandey, Indu Melkani, Bimlesh Kumar. Protective effect of Ethanolic extract of Terminalia arjuna bark against Ethylene Glycol induced Urolithiasis in male rats: In-Vitro and In-Vivo Evaluation. Research J. Pharm. and Tech. 2020; 13(12):6132-6139. doi: 10.5958/0974-360X.2020.01070.7


REFERENCES:
1.    S. Mitra, S. Gopumadhavan, M. Venkataranganna, and R. Sundaram, “Effect of cystone, a herbal formulation, on glycolic acid‐induced urolithiasis in rats,” Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, vol. 12, no. 5, pp. 372-374. 1998.
2.    A. Makasana, V. Ranpariya, D. Desai, J. Mendpara, and V. Parekh, “Evaluation for the anti-urolithiatic activity of Launaea procumbens against ethylene glycol-induced renal calculi in rats,” Toxicology reports, vol. 1, pp. 46-52. 2014.
3.    A. P. Evan, “Physiopathology and etiology of stone formation in the kidney and the urinary tract,” Pediatric Nephrology, vol. 25, no. 5, pp. 831-841. 2010.
4.    Y. Bayir, Z. Halici, M. S. Keles, S. Colak, A. Cakir, Y. Kaya, and F. Akçay, “Helichrysum plicatum DC. subsp. plicatum extract as a preventive agent in experimentally induced urolithiasis model,” Journal of ethnopharmacology, vol. 138, no. 2, pp. 408-414. 2011.
5.    S. K. Gupta, M. S. Baghel, C. Bhuyan, B. Ravishankar, B. Ashok, and P. D. Patil, “Evaluation of anti-urolithiatic activity of Pashanabhedadi Ghrita against experimentally induced renal calculi in rats,” Ayu, vol. 33, no. 3, pp. 429. 2012.
6.    R. Ganesamoni, and S. K. Singh, "Epidemiology of stone disease in Northern India," Urolithiasis, pp. 39-46: Springer, 2012.
7.    S. Jafar, L. Mehri, B. Hadi, and M. Jamshid, “The antiurolithiasic and hepatocurative activities of aqueous extracts of Petroselinum sativum on ethylene glycol-induced kidney calculi in rats,” Scientific Research and Essays, vol. 7, no. 15, pp. 1577-1583. 2012.
8.    M. M. Rane, and S. A. Mengi, “Comparative effect of oral administration and topical application of alcoholic extract of Terminalia arjuna bark on incision and excision wounds in rats,” Fitoterapia, vol. 74, no. 6, pp. 553-558. 2003.
9.    R. Chander, K. Singh, A. Khanna, S. Kaul, A. Puri, R. Saxena, G. Bhatia, F. Rizvi, and A. K. Rastogi, “Antidyslipidemic and antioxidant activities of different fractions ofTerminalia arjuna stem bark,” Indian Journal of Clinical Biochemistry, vol. 19, no. 2, pp. 141. 2004.
10.    K. Gauthaman, T. Mohamed Saleem, V. Ravi, P. Sita, and D. Niranjali, “Alcoholic Extract of Terminalia Arjuna Protects Rabbit Heart against Ischemic-Reperfusion Injury, Role of Antioxidant Enzymes and Heat Shock Protein,” World Acad Sci Eng Technol, vol. 18, pp. 488-98. 2008.
11.    H. A. Bachaya, Z. Iqbal, M. N. Khan, A. Jabbar, A. H. Gilani, and I.-u. Din, “In vitro and in vivo anthelmintic activity of Terminalia arjuna bark,” International Journal of Agriculture & Biology, vol. 11, pp. 273. 2009.
12.    A. Ram, P. Lauria, R. Gupta, P. Kumar, and V. Sharma, “Hypocholesterolaemic effects of Terminalia arjuna tree bark,” Journal of Ethnopharmacology, vol. 55, no. 3, pp. 165-169. 1997.
13.    M. Biswas, K. Biswas, T. K. Karan, S. Bhattacharya, A. K. Ghosh, and P. K. Haldar, “Evaluation of analgesic and anti-inflammatory activities of Terminalia arjuna leaf,” Journal of Phytology. 2011.
14.    M. A. Morshed, A. Uddin, A. Rahman, T. Hasan, S. Roy, A. Amin, R. Ahsan, and R. Islam, “In vitro antimicrobial and cytotoxicity screening of Terminalia arjuna ethanol extract,” International Journal of Biosciences, vol. 1, no. 2, pp. 31-38. 2011.
15.    P. Manna, M. Sinha, and P. C. Sil, “Aqueous extract of Terminalia arjuna prevents carbon tetrachloride induced hepatic and renal disorders,” BMC complementary and alternative medicine, vol. 6, no. 1, pp. 33. 2006.
16.    A. Chaudhary, S. Singla, and C. Tandon, “In vitro evaluation of Terminalia arjuna on calcium phosphate and calcium oxalate crystallization,” Indian journal of pharmaceutical sciences, vol. 72, no. 3, pp. 340. 2010.
17.    J. Burns, and B. Finlayson, “A proposal for a standard reference artificial urine in in vitro urolithiasis experiments,” Investigative urology, vol. 18, no. 2, pp. 167-169. 1980.
18.    G. P. Kumar, M. Arun, and K. Rishi, “Evaluation of Tinospora cordifolia for antiurolithiatic potential,” Journal of Pharmaceutical and Biomedical Sciences, vol. 9, no. 14, pp. 1-5. 2011.
19.    R. V. Karadi, N. B. Gadge, K. Alagawadi, and R. V. Savadi, “Effect of Moringa oleifera Lam. root-wood on ethylene glycol induced urolithiasis in rats,” Journal of ethnopharmacology, vol. 105, no. 1-2, pp. 306-311. 2006.
20.    K. Divakar, A. Pawar, S. Chandrasekhar, S. Dighe, and G. Divakar, “Protective effect of the hydro-alcoholic extract of Rubia cordifolia roots against ethylene glycol induced urolithiasis in rats,” Food and chemical toxicology, vol. 48, no. 4, pp. 1013-1018. 2010.
21.    A. Hodgkinson, “Determination of oxalic acid in biological material,” Clinical Chemistry, vol. 16, no. 7, pp. 547-557. 1970.
22.    N. Khan, J. Shinge, and N. Naikwade, “Antilithiatic effect of Helianthus annuus Linn. Leaf extract in ethylene glycol and ammonium chloride induced nephrolithiasis,” Int J Pharm Pharm Sci, vol. 2, no. 2010, pp. 181. 2010.
23.    S. Kaur, A. Rana, S. Gangwani, and R. Sharma, “Punica granatum attenuates sciatic nerve ligation induced-neuropathic pain,” International Journal of Pharmaceutical Sciences and Research, vol. 3, no. 2, pp. 509. 2012.
24.    G. Ellman, “Archs: Tissue sulfhydril groups,” Biochem Biophys, vol. 82, pp. 70-77. 1959.
25.    A. Ray, S. R. Chaudhuri, B. Majumdar, and S. K. Bandyopadhyay, “Antioxidant activity of ethanol extract of rhizome of Picrorhiza kurroa on indomethacin induced gastric ulcer during healing,” Indian Journal of Clinical Biochemistry, vol. 17, no. 2, pp. 44-51. 2002.
26.    M. Touhami, A. Laroubi, K. Elhabazi, F. Loubna, I. Zrara, Y. Eljahiri, A. Oussama, F. Grases, and A. Chait, “Lemon juice has protective activity in a rat urolithiasis model,” BMC urology, vol. 7, no. 1, pp. 18. 2007.
27.    A. Khajavi Rad, M.-A.-R. Hajzadeh, Z. Rajaei, M.-H. Sadeghian, N. Hashemi, and Z. Keshavarzi, “Preventive effect of Cynodon dactylon against ethylene glycol-induced nephrolithiasis in male rats,” Avicenna Journal of Phytomedicine, vol. 1, no. 1, pp. 14-23. 2011.
28.    A. Shukla, D. Mandavia, M. Barvaliya, S. Baxi, and C. Tripathi, “Anti-urolithiatic effect of cow urine Ark on ethylene glycol-induced Renal Calculi,” International braz j urol, vol. 39, no. 4, pp. 565-571. 2013.
29.    J. Fan, M. A. Glass, and P. S. Chandhoke, “Impact of ammonium chloride administration on a rat ethylene glycol urolithiasis model,” Scanning Microsc, vol. 13, no. 2-3, pp. 299-306. 1999.
30.    D. A. K. Chaitanya, M. Kumar, A. Reddy, N. Mukherjee, M. Sumanth, and A. Ramesh, “Anti urolithiatic activity of Macrotyloma uniflorum seed extract on ethylene glycol induced urolithiasis in albino rats,” J Innov Trends Pharm Sci, vol. 1, pp. 216-26. 2010.
31.    S. Bouanani, C. Henchiri, E. Migianu-Griffoni, N. Aouf, and M. Lecouvey, “Pharmacological and toxicological effects of Paronychia argentea in experimental calcium oxalate nephrolithiasis in rats,” Journal of ethnopharmacology, vol. 129, no. 1, pp. 38-45. 2010.
32.    A. Aggarwal, S. K. Singla, M. Gandhi, and C. Tandon, “Preventive and curative effects of Achyranthes aspera Linn. extract in experimentally induced nephrolithiasis.” 2012.
33.    P. Ashok, B. C. Koti, and A. Vishwanathswamy, “Antiurolithiatic and antioxidant activity of Mimusops elengi on ethylene glycol-induced urolithiasis in rats,” Indian journal of pharmacology, vol. 42, no. 6, pp. 380. 2010.
34.    S. B. SAYANA, V. R. CHAVAN, and R. MUDIUM, “Antilithiatic effect of Cissampelospareiraleaves in ammonium chloride and ethylene glycol induced urolithiasis in rats,” Asian J. Pharm. Clin. Res, vol. 7, pp. 103-106. 2014.

Recomonded Articles:

Author(s): Nimbalkar V.V., Pansare P.M., Nishane B.B.

DOI: 10.5958/0974-360X.2015.00310.8         Access: Open Access Read More

Author(s): Muhammad Hamdan, Noorhamdani AS, Masruroh Rahayu, Mohammmad Hasan Machfoed

DOI: 10.5958/0974-360X.2019.01020.5         Access: Open Access Read More

Author(s): Trophimus Gnanabagyan Jayakaran, Vignesh R, Shankar P

DOI: 10.5958/0974-360X.2019.00700.5         Access: Open Access Read More

Author(s): Raj Kumar R, Praveen D, Palani Shanmugasundaram

DOI: 10.5958/0974-360X.2016.00164.5         Access: Open Access Read More

Author(s): Vaseeha Banu T.S., Sandhya K.V., K.N. Jayaveera

DOI: Not Available         Access: Open Access Read More

Author(s): M. Sumithra, Avantika Prabhabanik

DOI: 10.5958/0974-360X.2017.00036.1         Access: Open Access Read More

Author(s): Barokar A.A., Wagh R.D., Baviskar D.T., Shaikh T.J.

DOI: Not Available         Access: Open Access Read More

Author(s): V Rajesh Babu, Syeda Rana Nikhat, P Nivethithai, SH Areefulla

DOI: Not Available         Access: Open Access Read More

Author(s): R. Suresh, D. Benitojohnson, C. Maheswari, R. Venkatnarayanan, R.Manavalan

DOI: 10.5958/0974-360X.2017.00128.7         Access: Open Access Read More

Author(s): Vijayalakshmi. S , Disalva. X, Chittaranjan Srivastava, Arun A

DOI: 10.5958/0974-360X.2019.00520.1         Access: Open Access Read More

Author(s): Tapan Kumar Giri, Saumya Mishra, Dulal Krishna Tripathi

DOI: Not Available         Access: Open Access Read More

Author(s): Karthikeyan D, Vijayakumar S, Pandey V.P, Nandha Kumar J, Sengottuvelu S, T. Sivakumar T.

DOI:         Access: Open Access Read More

Author(s): H.B. Pagar, U.P. Shinde, Y.S. Agrawal, S.D. Barhate, T.S. Luhade, R.O. Sonawane

DOI: Not Available         Access: Open Access Read More

Author(s): Rao Rekha, Goswami Anju, Shekhar Shishant, Mahant Sheefali, Nanda Sanju

DOI: Not Available         Access: Open Access Read More

Author(s): Loveleen Preet Kaur, Rajeev Garg, GD Gupta

DOI: Not Available         Access: Open Access Read More

Author(s): Mahadeva Rao U.S., Suganya M. Utharkar, C. Shanmuga Sundaram

DOI: 10.5958/0974-360X.2015.00307.8         Access: Open Access Read More

Author(s): Jagdale Swati C., Hable Asawaree A., Kuchekar Bhanudas S., Chabukswar Aniruddha R.

DOI: Not Available         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags