Archana Sharma, Prosanta Pal, Bapi Ray Sarkar, J. P Mohanty, Sonam Bhutia
Archana Sharma1*, Prosanta Pal2, Bapi Ray Sarkar2, J. P Mohanty1, Sonam Bhutia3
1Himalayan Pharmacy Institute, Majhitar, Sikkim University, East Sikkim-737136.
2Department of Pharmaceutical Technology, University of North Bengal, West Bengal, India-734013.
3Government Pharmacy College, Government of Sikkim, Sikkim University, Sajong, Rumtek East Sikkim-737135.
Volume - 13,
Issue - 12,
Year - 2020
The present study was undertaken to evaluate the pharmacognostical parameters, formulation, standardization and hypoglycaemic effect of herbal preparation in alloxan induced diabetic rats. The physicochemical properties of polyherbal finished product were: Specific gravity (1.03 at 25°C), pH of 10%v/v solution (4.543), solid content (7% w/v), viscosity (4.59 cp), surface tension (43.37 dynes/cm) and refractive index (1.37). The colour (brown), odour (sweet aromatic), and taste (sweet) of polyherbal formulation was satisfactory in physical appearance. The study was also aimed to evaluate the antidiabetic activity of herbal formulation in alloxan induced diabetic rats model. The results of the body weight and blood glucose level of control, diabetic control group, standard group (Glibenclamide 5mg/kg)-standard drug used and two different doses of trial formulation (600mg and 300mg/kg)-summarised in Table No 1 and Table No 2 respectively. The initial body weight of Herbal formulation low dose and high dose were 123.33±5.78 and 185±8.38 respectively and after 11 days of treatment the bodyweight was decreased to 119.33±8.45 and1 70.33±7.79 respectively. Two different doses of the herbal formulation (low dose and high dose) i,e 123.33±5.78 and 185±8.38 blood glucose level were studied in two different groups of animals. Both groups showed a significant decrease of blood glucose level on alloxan induced diabetic rats when compared to control group. The low dose result shows for respective day 3: 5.01±0.21, day 7: 33.23±1.70 and day11: 63.20±2.46 and for high doses for day 3: 6.59±0.34, day 7: 35.26±1.84 and day 11: 65.63±2.46. Both groups showed a significant decrease of blood glucose level on alloxan induced diabetic rats when compared to control group. The present study showed that the investigation of the polyherbal formulation containing five herbs has a potent anti diabetic effect which could be used in the management of diabetes mellitus effectively.
Cite this article:
Archana Sharma, Prosanta Pal, Bapi Ray Sarkar, J. P Mohanty, Sonam Bhutia. Preparation, Standardisation and Evaluation of Hypoglycaemic effect of Herbal Formulation containing five Ethnomedicinal plants in Alloxan-Induced Hyperglycemic Wistar Rats. Research J. Pharm. and Tech. 2020; 13(12):5987-5992. doi: 10.5958/0974-360X.2020.01044.6
Archana Sharma, Prosanta Pal, Bapi Ray Sarkar, J. P Mohanty, Sonam Bhutia. Preparation, Standardisation and Evaluation of Hypoglycaemic effect of Herbal Formulation containing five Ethnomedicinal plants in Alloxan-Induced Hyperglycemic Wistar Rats. Research J. Pharm. and Tech. 2020; 13(12):5987-5992. doi: 10.5958/0974-360X.2020.01044.6 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-12-59
1. Ali, M. (2009). "Present status of herbal medicines in India." Journal of herbal medicine and toxicology 3(2): 1-7.
2. Avise, J. C. (2004). The hope, hype, and reality of genetic engineering: Remarkable stories from agriculture, industry, medicine, and the environment, Oxford University Press 5(3):56-59.
3. Bussmann, R. W. (2002). Ethnobotany and biodiversity conservation. Modern trends in applied terrestrial ecology, Springer 6(4): 343-360.
4. Casey, G. (2011). "The sugar disease—understanding type 2 diabetes mellitus." Kai Tiaki Nursing New Zealand 17(2): 16-21.
5. Jamal, A. R. M. (2011). Ocimum sanctum L.: A review of phytochemical and pharmacological profile 2(3):76-84.
6. Pandey, G. and S. Madhuri (2010). "Pharmacological activities of Ocimum sanctum (tulsi): a review." Int J Pharm Sci Rev Res 5(1): 61-66.
7. Ashafa, A. O. T. (2012). Toxicity profile of ethanolic extract of Azadirachta indica stem bark in male Wistar rats. Asian Pacific Journal of Tropical Biomedicine 811-817.
8. Pandey, G. (2014). Evaluation of phytochemical, antibacterial and free radical scavenging properties of Azadirachta indica (Neem) leaves. International Journal of Pharmacy and Pharmaceutical Sciences 6(2): 444-447.
9. Omezzine, F., et al. (2012). Induction and flow cytometry identification of mixoploidy through colchicine treatment of Trigonella foenum-graecum L. African Journal of Biotechnology 11(98): 16434-16442.
10. Yadav, R., et al. (2011). A pharmacognostical monograph of trigonella foenum- graecum seeds. International Journal of Pharmacy and Pharmaceutical Sciences 3(5): 442-445.
11. Grover, J.K. (2000). Anti-hyperglycemic effect of Eugenia jambolana and Tinospora cordifolia in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism. Journal of Ethnopharmacology 73: 461–470.
12. Nasreen, S. (2010). Assessment of quality of Tinospora cordifolia (wild.) miers. (Menispermaceae): pharmacognostical and phyto-physicochemical profile. International Journal of Comprehensive Pharmacy 5(03) 1-4.
13. Baskaran, K. et al., (1990). Antidiaretic effect of a leaf extract from Gymnema sylvestre in non-insulin-dependent diabetes mellitus patients. Journal of Ethnopharmacology 30: 295–305.
14. Patil, R. (2011). Current status of Indian medicinal plants with antidiabetic potential: a review. Asian Pacific Journal of Tropical Biomedicine. S291-S298.
15. Kumar, G.,(2013). "Natural Product and Health-A Review on All Aspects." Current Research in Pharmaceutical Sciences 3(3): 68-79.
16. Akbar, S. (2014). Pharmacognostic studies of stem, roots and leaves of Malva parviflora L. Asian Pacific Journal of Tropical Biomedicine 4(5): 410-415.
17. Kumar D, Kumar K, Kumar S, Kumar T, Kumar A, Prakash O. Pharmacognostic evaluation of leaf and root bark of Holoptelea integrifolia Roxb. Asian Pac J Trop Biomed 2012; 2(3): 169–175.
18. Anonymus. Pharmacopoeia of India. Vol. 2. New Delhi: The Controller of Publications; 1996. P. A47- A54.
19. Bhutia S, Uriah T, Mohanty J P, Pal P, Kakoti B B. Physicochemical evaluation, gc-ms and antibacterial activity of essential oil of the fruit peels of Citrus macroptera montr. of Meghalaya north east India. World J. Ph Life Sci 2019; 5(4): 108-115.
20. Stanley Irobekhian Reuben Okoduwa, Ismaila A Umar, Dorcas B James, Hajara M Inuwa, and James D Habila, Evaluation of extraction protocols for anti-diabetic phytochemical substances from medicinal plants, 2016 ; 7(20): 605–614.
21. Bitasta, M. (2011). Comparative Standardization and Physicochemical Evaluation of the Leaves of Stevia rebaudiana Bertoni from Different Geographical Sources. Pharmacognosy Journal 3(25): 21-26.
22. Prasad S.K. (2012). Physicochemical standardization and evaluation of in-vitro antioxidant activity of Aconitum heterophyllum Wall. Asian Pacific Journal of Tropical Biomedicine. S526-S531.
23. Lipnick, R.L. et al., (1995). Comparison of Conventional LDs0, Toxicity the Up-and -Down and Fixed-Dose Acute Procedures. Food and Chemical Toxicology 33(3): 223-23.
24. BRUCE R. D. (1985). An Up-and-Down Procedure for Acute Toxicity Testing. Fundamental and applied toxicology 5: 15 1- 157.
25. Oliveira, H.C. et al., (2008). Antidiabetic activity of Vatairea macrocarpa extract in rats. Journal of Ethnopharmacology 115: 515–519.
26. Esampally Sucharitha, Mamidala Estari, Evaluation of antidiabetic activity of medicinal plant extracts used by tribal communities in rural areas of Warangal district, Andhra Pradesh, India, Biology and Medicine, 5: 20–25, 2013