Author(s): Nihad Abdulhussain Jafar, Bashar Sadeq Noomi, Akeel Hussain Ali Al-Assie, Shadman Tariq Sadiq

Email(s): shadmantsm@tu.edu.iq

DOI: 10.5958/0974-360X.2020.01022.7   

Address: Nihad Abdulhussain Jafar1, Bashar Sadeq Noomi2, Akeel Hussain Ali Al-Assie3, Shadman Tariq Sadiq4*
1,2Department of Microbiology, College of Veterinary Medicine, Tikrit University.
3Department of Biology, College of Science, Tikrit University, Iraq.
4Department of Industrial and Fundamental Microbiology, Institute of Science, Ege University, 35040 Izmir, Turkey.
*Corresponding Author

Published In:   Volume - 13,      Issue - 12,     Year - 2020


ABSTRACT:
Live attenuated Brucella melitensis Rev.1 strain is currently used in Iraq against caprine and ovine brucellosis. Vaccination with Rev.1 may lead to the adverse effects of strain persistence in the vaccinated animals and its occasionally spread horizontally. Moreover, in some countries it was shown that the vaccine strain caused human infection, demonstrating the zoonotic hazards of its virulence. Thirty Sheep milk samples and 30 Goat milk samples were taken from animals given a positive result with rose Bengal test. Also 12 Vaginal swabs from aborted ewes and 20 Vaginal swabs from aborted goats were taken in period didn't exceed 10 days after the abortion. At the same time, 50 blood samples taken from patients with Malta fever. The samples cultured on trypton soya broth and then sub-cultured on Brucella Basel agar. Biochemical test, PCR- RELP, and DNA sequencing were used to distinguish Vaccine Rev.1 strain from field strains. Out of 92 samples from goats and sheep, Brucella isolated from 43 (46.7%) case. The results revealed that vaginal discharges are better than milk samples for isolation of the Brucella. In human, the bacteria isolated from 11 (22%) of Malta fever patients. PCR-RELP and DNA sequencing revealed that Rev.1 strain isolated from 8 (21%) animals and 1 (11.1%) of human patients.


Cite this article:
Nihad Abdulhussain Jafar, Bashar Sadeq Noomi, Akeel Hussain Ali Al-Assie, Shadman Tariq Sadiq. The use of DNA Sequencing to confirm the first report of Rev.1 strain isolated from human brucellosis in Iraq. Research J. Pharm. and Tech. 2020; 13(12):5866-5870. doi: 10.5958/0974-360X.2020.01022.7


REFERENCES:
1.    Addis M. Public health and economic importance of brucellosis: A review. Public Health. 2015; 5(7): 68-84.
2.    Ko J and Splitter GA. Molecular host-pathogen interaction in brucellosis: current understanding and future approaches to vaccine development for mice and humans. Clinical Microbiology Reviews. 2003; 16(1): 65-78.‏
3.    Moriyón I, Grilló MJ, Monreal D, González D, Marín C, López-Goñi I and Blasco JM. Rough vaccines in animal brucellosis: structural and genetic basis and present status. Veterinary Research. 2004; 35(1): 1-38.‏
4.    Radostits OM, Gay C, Hinchcliff KW and Constable PD. A textbook of the diseases of cattle, sheep, goats, pigs and horses. Veterinary Medicine 10th edition Bailliere, Tindall, London, UK.2007.
5.    Alton GG, Jones LM, Angurs RD and Verger JM. Techniques for the Brucellosis Laboratory. INRA. Paris, France.1988.
6.    WHO and OIE, Brucella melitensis in Eurasia and the middle east. FAO technical meeting in collaboration with WHO and OIE Rome, May 2009.
7.    Al Zahawi S. Confirmation of the Existence of Undulant Fever in Iraq. Bulletin de l'Office International d'Hygiene Publique.1938; 30:1559-62.‏
8.    Karaawi S. brucellosis in Iraq. J. Bio. Innov. 2016; 5(2): 285-293.‏
9.    Zinsstag J, Schelling E, Roth F, Bonfoh B, De Savigny D andTanner M. Human benefits of animal interventions for zoonosis control. Emerging Infectious Diseases. 2007; 13(4): 527.‏
10.    Nicoletti P. Vaccination against Brucella. Advances in Biotechnological Processes. 1990; 13: 147-168.‏
11.    Ashford DA, Di PietraJ, Lingappa J, Woods C, Noll H, Neville B and Perkins BA. Adverse events in humans associated with accidental exposure to the livestock brucellosis vaccine RB51. Vaccine. 2004; 22(25): 3435-3439.‏
12.    Elberg SS and Faunce Jr K. Immunization against Brucella infection VI. Brucella melitensis: Immunity Conferred on Goats by a Nondependent Mutant from a Streptomycin-Dependent Mutant Strain of1. Journal of Bacteriology.1957; 73(2): 211.‏
13.    Elberg SS and Meyer KF. Caprine immunization against brucellosis: A summary of experiments on the isolation, properties and behavior of a vaccine strain. Bulletin of the World Health Organization. 1958;19(4): 711.‏
14.    Bardenstein S, Mandelboim M, Ficht TA, Baum M and Banai M. Identification of the Brucella melitensis vaccine strain Rev. 1 in animals and humans in Israel by PCR analysis of the PstI site polymorphism of its Omp2 gene. Journal of Clinical Microbiology.2002; 40(4): 1475-1480.‏
15.    Blasco JM. A review of the use of B. melitensis Rev 1 vaccine in adult sheep and goats. Preventive Veterinary Medicine.1997; 31(3-4): 275-283.‏
16.    Alton GG, Jones LM and Pietz DE. Laboratory Techniques in Brucellosis. 2nd ed. World Health Organization. Geneva. 1975.
17.    Quinn PJ, Markey BK, Carter ME, Donnelly WJC and Leonard F C. Veterinary Microbiology and Microbial Disease. 1st ed. Blackwell Science Ltd; London. 2002.
18.    Romero C, Pardo M, Grillo M, Diaz R, Blasco J, Lopez-Goni I (1995) Evaluation of PCR and indirect enzyme linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle. J. Clin. Microbiol; 33: 3198-3200.
19.    Marin CM, Alabart JL and Blasco JM. Effect of antibiotics contained in two brucella selective media on growth of Brucella abortus, B. melitensis and B. ovis. J. Clin. Microbiol. 1996; 34: 426-428.
20.    Pouillot R, Garin-Bastuji B, Gerbier G, Coche Y, Can C, Dufour B and Moutou F. The brucellin skin test as a tool to differentiate false positive serological reactions in bovine brucellosis. Vet. Res.1997; 28: 365-374.
21.    Corbe MJ and MacMillan A. Bovine brucellosis. In “Manual of standards for diagnostic tests and vaccines”. Office International des Epizooties. Paris. 2000.
22.    Hamdy MER, Amin AS. Detection of Brucella species in the milk of infection cattle, sheep, goat and camels by PCR. Vet. J2002; 163:168-174.
23.    OIE. caprine and ovine brucellosis. In: OIE manual of diagnostic tests and vaccines for terrestrial animals. 5th ed. 2004.
24.    Blasco JM. A review of the use of B. melitensis Rev.1 vaccine in adult sheep and goats. Prev. Vet. Med1997; 31:275-283.
25.    Hadad JJ and Al-azawy Z S. Incidence of brucellosis in sheep and goats in Ninevah Province. Iraqi J. Vet. Sci1990; 4(1): 27-33.
26.    Mullis KB and Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. In Methods in Enzymology (Vol. 155, pp. 335-350). Academic Press.‏ 1987.
27.    Nashwa MH, Hoda MZ and Adawy SS. Identification and Differentiation of Brucella melitensis Rev.1 Vaccine and B. Melitensis Biovar 3 Field Isolates in Egypt by Serological and PCR-RELP Techniques. Journal of Applied Sciences Research. 2007; 3(9): 841-847.

Recomonded Articles:

Author(s): Adithya Chandrashekar, Anuraag Muralidharan, Ananthamurthy Koteshwara, Angel Treasa Alex, V. M. Subrahmanyam

DOI: 10.5958/0974-360X.2019.00791.1         Access: Open Access Read More

Author(s): Saba Th Mousa, Sufian Sa Saleh, Nasr Ta Mohammed

DOI: 10.5958/0974-360X.2019.00630.9         Access: Open Access Read More

Author(s): Sara H. Mohamed, Mary S. Khalil, Mona I. Mabrouk, Mahmoud S.M. Mohamed

DOI: 10.5958/0974-360X.2020.00542.9         Access: Closed Access Read More

Author(s): Payel Mitra, Arun Kumar Dutta, Narayan Chandra Mandal, Krishnendu Acharya

DOI: 10.5958/0974-360X.2019.00737.6         Access: Open Access Read More

Author(s): S. Ivo Romauld, R. Venkataraghavan, D. Yuvaraj, Ishwarya Devi. V, Hashika. S

DOI: 10.5958/0974-360X.2019.00725.X         Access: Open Access Read More

Author(s): Nihad Abdulhussain Jafar, Bashar Sadeq Noomi, Akeel Hussain Ali Al-Assie, Shadman Tariq Sadiq

DOI: 10.5958/0974-360X.2020.01022.7         Access: Closed Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags