Author(s): N. R. Adawiyah N. Zulkifeli, Haniza Hanim Mohd Zain, Ismail Zainol, Nur Hidayat Che Musa

Email(s): haniza@fsmt.upsi.edu.my

DOI: 10.5958/0974-360X.2020.01020.3   

Address: N. R. Adawiyah N. Zulkifeli, Haniza Hanim Mohd Zain*, Ismail Zainol, Nur Hidayat Che Musa
Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjung Malim, Perak Darul Ridzuan, Malaysia.
*Corresponding Author

Published In:   Volume - 13,      Issue - 12,     Year - 2020


ABSTRACT:
The purpose of this study was to determine the properties of hydrolysed collagen from Orechromis mossambicus’s scales. The properties determination includes pH measurement, macronutrient evaluation, and molecular weight determination. Meanwhile, the effect of hydrolysed collagen at various concentrations on the viability of normal skin fibroblast primary cells has also examined. The pH value of this hydrolysed collagen was in the vicinity of 5. The moisture content, ash content, fat content, and protein content showed 3.65%, 0.79%, 0.22% and 63.1%, respectively. The polypeptide profile obtained in the SDS-PAGE analysis was between 17.75 to 23.50 kDa. Besides, 4mg/mL until 10mg/mL of hydrolysed collagen concentrations increased the viability of normal skin fibroblast primary cells.


Cite this article:
N. R. Adawiyah N. Zulkifeli, Haniza Hanim Mohd Zain, Ismail Zainol, Nur Hidayat Che Musa. The properties of Hydrolysed Collagen from Oreochromis mossambicus’s scale and their effect towards Cell viability. Research J. Pharm. and Tech. 2020; 13(12):5855-5860. doi: 10.5958/0974-360X.2020.01020.3


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