Author(s): Gopalasatheeskumar K, Kalaichelvan VK, Ariharasivakumar G, Sengottuvel T, Sanish Devan V

Email(s): gskpungai@gmail.com

DOI: 10.5958/0974-360X.2020.01019.7   

Address: Gopalasatheeskumar K1*, Kalaichelvan VK1, Ariharasivakumar G2, Sengottuvel T2, Sanish Devan V2
1Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu - 608002, India.
2Department of Pharmacology, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore -641048, Tamil Nadu, India (Affiliated to The Tamil Nadu Dr. M.G.R. Medical University).
*Corresponding Author

Published In:   Volume - 13,      Issue - 12,     Year - 2020


ABSTRACT:
Background: Diabetes is a group of metabolic diseases characterized by high levels of blood sugar (hyperglycemia). Many of the antidiabetic drugs available to treat diabetes but they have the side effects so that finding of alternatives is important in diabetic research. Cucumis melo var agrestis (CMA) is under the family of Cucurbitaceae, many of the cucurbitacae family plants has reported as antidiabetic activity but still there is no reports on antidiabetic activity on CMA. Aim and Objectives: The present study is aimed to that evaluation of in vitro antidiabetic activities of hydroalcoholic leaf extract of Cucumis melo var agrestis (HALEC). Methods: The antidiabetic activity was evaluated by hemoglobin glycosylation Inhibition assay, Alpha amylase inhibition assay, alpha glucosidase inhibitory assay, Glucose uptake in yeast cells, Glucose diffusion assay and glucose adsorption capacity. Results: Our results revealed that HALEC has potent antidiabetic activities it was compared with standard acarbose and metformin. Conclusion: From the results of this research the HALEC has the antidiabetic activity and further study needed to animal studies and finding of exact mechanism.


Cite this article:
Gopalasatheeskumar K, Kalaichelvan VK, Ariharasivakumar G, Sengottuvel T, Sanish Devan V. In vitro Antidiabetic activity of hydroalcoholic leaf extract of Cucumis melo var agrestis (Cucurbitaceae). Research J. Pharm. and Tech. 2020; 13(12):5851-5854. doi: 10.5958/0974-360X.2020.01019.7


REFERENCES:
1.    Maitra A, Abbas AK. Endocrine system. Robbins and Cotran Pathologic basis of disease (7thedtn). Saunders, Philadelphia. 200; 1156-1226.
2.    Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care. 2004; 27:1047-1053.
3.    Patlak M. New weapons to combat an ancient disease: treating diabetes.  FASEB Journal.2002; 16:18-53.
4.    Switi BG, Krishna MG, Sandhya RM. Phytochemicals for Diabetes Management. Pharmaceutical Crops. 2014; 5:11-28
5.    Gopalasatheeskumar K, Ariharasivakumar G, Sengottuvel T et al. Confirmation of Best Method for Detection of Alpha Amylase Activity of Cucumis Melo Var Agrestis (Wild Musk Melon). Journal. Journal of Advanced Research in Biochemistry and Pharmacology. 2019; 2(1): 1-3.
6.    Kripa S, Sumit KS, Durgesh K, Salil V, Abhishek G, Sujith R et al., Cucumismelo ssp. agrestis var. agrestis ameliorates high fat diet induced dyslipidemia in syrian golden hamsters and inhibits adipogenesis in 3T3-L1 adipocytes. Pharmacognosy Magazine. 2015; 11(4):501-510.
7.    Gopalasatheeskumar K,  Parthiban S, Manimaran T, Boopathi T. Phytochemical Screening on Various Extracts (Benzene, Ethanolic and Aqueous) of Stem Parts of Zanthoxylum Rhetsa (Roxb.) Dc. International Journal of Universal Pharmacy and Bio Sciences. 2017; 6(2):79-91.
8.    Mirunalini, S. and Vaithiyanathan, V. Assessment of antioxidant potential and acute toxicity studies of whole plant extract of Pergulariadaemia (Forsk). Toxicology International. 2015; 22(1): 54.
9.    Gopalasatheeskumar K, Ariharasiva Kumar G, Sengottuvel T, Sanish Devan V, Srividhya V. Quantification of Total Phenolic and Flavonoid content in leaves of Cucumis melo var agrestis using UV- spectrophotometer. Asian Journal of Research in Chemistry. 2019; 12(6):335-337.
10.    Hossan SJ, Sayed M, Aoshima H. Antioxidative and anti α-amylase activities of four wild plants consumed by nomads in Egypt. Oriental Pharmacy and Experimental Medicine. 2009; 9(3):217-224.
11.    Yuvaraja K R, Santhiagu A, Jasemine S, Gopalasatheeskumar K. Hepatoprotective activity of Chloroform and Ethyl acetate extract of Dipteracanthus patulus against Paracetamol induced Hepatotoxicity in rats through Antioxidant mechanism. Research Journal of Pharmacy and Technology. 2020; 13(1):203-208.
12.    Xiao Z, Storms R, Tsang A. A quantitative starch iodine method for measuring alpha-amylase and glucoamylase activities. Analytical Biochemistry. 2006; 351(1):146-148.
13.    Afolakemi AA, Anofi OTA. An in vitro evaluation of the antioxidant and antidiabetic potential of Sutherlandiamontana E. Phillips & R.A. Dyer leaf extracts. Asian Pacific Journal of Tropical Biomedicine. 2017; 7(9):765–772.
14.    Sindhu SN, Vaibhavi K, Anshu M. Evaluation of In Vitro Anti diabetic Activity of Selected Plant Extracts. International Journal of Pharmaceutical Science Invention. 2013; 2(4):12-19.
15.    Radhika S, Senthil KR, Sindhu S, Sagadevan E, Arumugam P. Phytochemical Investigation and Evaluation of Antihyperglycemic Potential of Premna Corymbosa. International Journal of Pharmacy and Pharmaceutical Sciences. 2013; 5(4):352-356.
16.    Bhandari MR, Anurakkun NJ, Hong G, Kawabata J. a-Glucosidase and a-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergeniaciliata, Haw). Food Chemistry. 2008; 106:247-252.
17.    Pattan Shashikant R, Kekare Prajact, NS Dighe, SB Bhawar, Nikalje Ana, Pati Ashwini, MB Hole. Synthesis and Evaluation of Some New Thiazolidinedione Derivatives for Their Antidiabetic Activities. Asian Journal of Research in Chemistry. 2009; 2(2): 123-126.
18.    Radhika K, Kumaravel B, Thamizhiniyan V, Subramanian. Biochemical evaluation of antidiabetic activity of Piper betel leaves extract in alloxan-induced diabetic rats. Asian Journal of Research in Chemistry. 2013; 6(1): 76-82.
19.    Adhikary S, Kandar CS, Haldar PK, Basu A, Choudhury S. Preclinical Evaluation of Antidiabetic Effect of Pedilanthus tithymaloides Extracts in Streptozotocin (STZ) Induced Diabetic Rats. Research Journal of Pharmacy and Technology. 2010; 3 (4): 1132-1133.
20.    Wate SP, Duragkar N, Tajne MR, Jadhav SS. Study of Antidiabetic Activity of Cow Urine and Its Concentrate. Research Journal of Pharmacy and Technology. 2012; 5(6): 789-790.
21.    Godavari A, Amutha K. In vitro Antidiabetic Activity of Garcinia mangostana by Enzymatic Inhibition Assay. Research Journal of Pharmacy and Technology. 2017; 10(2): 508-512.
22.    Benito Johnson D, Akhil Raj R. Venkatanarayan. Antidiabetic Activity and Toxicity Studies of Ethanolic Extract of Polialthia longifolia Roots. Research Journal of Pharmacy and Technology. 2017; 10(5): 1305-1312.
23.    Vidyasabbani, Ramesh Alluri, Shobharani Satla. Comparative In vitro antidiabetic and antioxidant activity of Pulicaria wightinia, Curcuma inodora, Derris scandens leaf extracts. Research Journal of Pharmacy and Technology. 2017; 10(6): 1615-1620.
24.    Sundarrajan T, Velmurugan V, Srimathi R. Phytochemical Evaluation and In Vitro Antidiabetic Activity of Ethanolic extract of Alternanthera ficodia Linn. Research Journal of Pharmacy and Technology. 2017; 10(9): 2981-2983.
25.    Lokhande VY, Yadav AV. In vitro Antioxidant and Antidiabetic Activity of Supercritical Fluid extract of Leaves Ocimum sanctum L. Research Journal of Pharmacy and Technology. 2018; 11(12): 5376-5378.
26.    Amutha K, Aishwarya S. Evaluation of Antibacterial and Antidiabetic Activity and Phytochemical Analysis of Syzygium cumini (L.) Skeels. Seed. Research Journal of Pharmacology and Pharmacodynamics. 2010; 2(5): 348-350.

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