Author(s): S. Vijayalakshmi, K. G. Kripa, Suganthi V.

Email(s): kgkripa.sls@velsuniv.ac.in

DOI: 10.5958/0974-360X.2020.00988.9   

Address: S. Vijayalakshmi1, K. G. Kripa1*, Suganthi V.2
1Department of Biochemistry, School of Life Sciences, Vels Institute of Science Technology and Advanced Studies (VISTAS), Pallavaram, Chennai- 600 117, India.
2Department of Home Science, Anna Adharsh College for Women, Chennai - 600040, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 12,     Year - 2020


ABSTRACT:
This study aimed to establish the safety profile of hydroalcoholic extract of Blepharis maderaspatensis (HAEBM) by performing acute and sub-acute oral toxicity studies in Wistar Albino rats of both sexes in accordance with OECD guidelines. In the acute toxicity study (OECD 423), first group (?? = 6) served as control, received 0.2% CMC while the second group (?? = 6) was treated with HAEBM at the dose of 2g/kg body weight p.o. Mortality was checked for 14days. In the sub-acute toxicity studies (OECD 407), the first group served as control while the other three groups (?? = 6) received oral administration of HAEBM (100, 200, and 400 mg/kg b.wt./day respectively) for 28 days. Animals were sacrificed on 29th day. Body weight, hematological, biochemical parameters and histopathological changes were evaluated. Statistical Analysis was performed using Dunn’s post hoc test. Acute toxicity studies indicated nil mortality. Sub-acute toxicity studies proved HAEBM to be non-toxic as it did not produce significant changes in organ weight, hematological parameters, and biochemical parameters. This study depicts that acute exposure of 2g/kg b.wt./day and sub-acute administration of HAEBM for 28 days proved safe and non-toxic and hence may be suitable for therapeutic use.


Cite this article:
S. Vijayalakshmi, K. G. Kripa, Suganthi V. Toxicological Study of Hydroalcoholic Extract of Blepharis maderaspatensis (L.) Heyne ex Roth in Wistar Albino Rats. Research J. Pharm. and Tech. 2020; 13(12):5673-5679. doi: 10.5958/0974-360X.2020.00988.9


REFERENCES:
1.    Bussmann RW. 2013. The globalization of traditional medicine in Northern Peru: from shamanism to molecules. Evidence-based complementary and alternative medicine: eCAM. [ONLINE] Available at: http://doi.org/10.1155/2013/291903. [Accessed o3 January 2018].
2.    Tilburt JC, Kaptchuk TJ. Herbal medicine research and global health: an ethical analysis. Bull World Health Organ 2008; 86(8): 594-9.
3.    Joos S, Glassen K, Musselmann B. Herbal medicine in primary healthcare in Germany: The patient’s perspective. Evidence-based complementary and alternative medicine. eCAM 2012;2012: 01-10.
4.    Suryavanshi SA, Kadam KS, Raina P, Nimbargi R, Pandit VA, Ghanekar RK. Evaluation of acute and sub-acute toxicity of a standardized polyherbal formulation (Hc9): an in vivo study. IJPPS 2015;7(11): 110-17.
5.    Madrigal-Santillán E, Madrigal-Bujaidar E, Álvarez-González I, Sumaya-Martínez MT, Gutiérrez-Salinas J, Bautista M, Morales-González A et al. Review of natural products with hepatoprotective effects. World J Gastroenterol 2014; 20(40): 14787–804.
6.    Prasad R, Lawania RD, Manvi, Gupta R. Role of herbs in the management of asthma. Phcog Rev 2009;3: 247-58. Available at: http://www.phcogrev.com/text.asp?2009/3/6/247/59524. [Accessed 13 January 2018].
7.    Mashour NH, George IL, Frishman WH. Herbal medicine for the treatment of cardiovascular disease clinical considerations. Arch Intern Med 1998;158(20): 2225-34.
8.    Bhowmik D, Sampath Kumar KP, Tripathi P, Chiranjib. Traditional herbal medicines: an overview. Archives of Applied Science Research 2009;1(2): 165-77. Available at: www.scholarsresearchlibrary.com. [Accessed 09 January 2018].
9.    Chapter 1. Wachtel-Galor S, Benzie IFF. Herbal Medicine: An introduction to its history, usage, regulation, current trends, and research needs. In: Benzie IFF, Wachtel-Galor S, editors. Herbal Medicine: Biomolecular and Clinical Aspects. 2nd edition. Boca Raton (FL): CRC Press/Taylor and Francis; 2011.
10.    Parasuraman S. Toxicological screening. J Pharmacol Pharmacother 2011;2(2): 74–9.
11.    Ogbuehi IH, Ebong OO, Obianime AW. Oral acute toxicity (LD50) study of different solvent extracts of Abrusprecatorius linn leaves in Wistar rats. Euro J Exp Bio 2015;5(1): 18-25.
12.    Toxicological Studies. Institute for industrial research and toxicology (IIRT) [ONLINE]. Available at: http:// www.toxicityindia.org/category/toxicology/sub-acute-toxicity-studies.html. [Accessed 12 March 2018].
13.    Vijayalakshmi S, Kripa KG. Therapeutic uses of plants of genus blepharis- a systematic review. Int J Pharm Bio Sci 2016;7(4): 236 – 43.
14.    Muthukrishnan S, Indupriya S, Subha P. Preliminary Phytochemical screening and antioxidant potential of Blepharis maderaspatensis (L.) B. Heyne Ex Roth Leaf. WJPPS 2014;3(5): 658-68.
15.    Sowemimo A, Onakoya M, Muyiwa S. Fageyinbo, Fadoju T. Studies on the anti-inflammatory and anti-nociceptive properties of Blepharis maderaspatensis leaves. Rev bras Farmacogn 2013;23(5): 830-35.
16.    Kripa KG, Chamundeeswari D, Thanka J. Acute and sub-acute toxicity evaluation of ethanolic extract of Leucasaspera (Lamiaceae) in experimental rats. Int J Drug Dev Res 2011;3(3): 339-47.
17.    Chinedu E, Arome D, Ameh, FS. A new method for determining acute toxicity in animal models. Toxicology International 2013;20(3): 224–26. Available at: http://doi.org/10.4103/0971-6580.121674. [Accessed 22 February 2018].
18.    Subha D, Geetha N. Evaluation of acute toxicity of the methanolic extract of Tanacetumparthenium L. in Albino Wistar rats. J SciInno Res 2017; 6(3): 113-15.
19. Santosh BN, Pravin CD. Acute and sub-acute oral toxicity assessment of the polyherbal formulation in Albino Wister rats. IJPPS 2016;8(7): 311-16.
20.    Ali R, Ali R, Jaimini A, Nishad DK, Mittal G, Chaurasia OP, Kumar R, Bhatnagar A, Singh SB. Acute and sub acute toxicity and efficacy studies of Hippophaerhamnoides based herbal antioxidant supplement. Indian J Pharmacol 2012;44: 504-8.
21.     Santosh NB, Pravin DC. Acute and sub-acute oral toxicity assessment of the polyherbal formulation in Albino Wister rats. Int J Pharm PharmSci 2016;8(7): 311-16.
22.    Etim NN, Williams ME, Akpabio U, Offiong EEA. Haematological parameters and factors affecting their values. Agricultural Science 2014;2(1): 37-47.
23.    Dar SA, Ghazanfar K, Akbar S, Masood A, Nazir T, Siddiqui KM, Pawankumar. acute and subacute oral toxicity studies of Deedan- a Unani drug in Albino rats. J App Pharm Sci 2015;5(04): 107-14.
24.    Temba SG, Erasto P, Swai DE, Chacha M. Toxicological evaluation of Commiphora swynnertonii stem bark exudates on mice and rats. J. Bio. andEnv. Sci, 2017;11(1): 321-29.
25.    Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol 2014;4: 01-10.
26.    World Health Organization. General guidelines for methodologies on research and evaluation of traditional medicine. Geneva: 2000. Available at: http://apps.who.int/iris/bitstream/10665/66783/1/ WHO_EDM_TRM_2000.1.pdf. [Accessed 20 February 2018].
27.    Bello I, Bakkouri AS, Tabana YM, Al-Hindi B, Al-Mansoub MA, Mahmud R, Asmawi MZ. Acute and sub-acute toxicity evaluation of the methanolic extract of Alstoniascholaris stem bark. Med Sci (Basel) 2016;4(1): 4.
28.    Moreira DL, Teixeira SS, Monteiro MHD, De-Oliveira ACAX, Paumgartten FJR. Traditional use and safety of herbal medicines. Rev bras Farmacogn 2014; 24( 2 ): 248-57.
29.    National Health and Medical Research Council (NHMRC)- Australian Group, 2008.Guidelines to promote the wellbeing of animals used for scientific purposes- The assessment and alleviation of pain and distress in research animals , NHMRC publications. Available at: http://www.nhmrc.gov.au/index.htm. [Accessed 22 January 2018].
30.    Piao Y, Liu Y, Xie X. Change trends of organ weight background data in spraguedawley rats at different ages. J ToxicolPathol 2013;26: 29-34.
31.    Hall AP, Elcombe CR, Foster JR, Harada T, Kaufmann W, Knippel A et al. Liver hypertrophy a review of adaptive (adverse and non-adverse) changes—conclusions from the 3rd international ESTP Expert Workshop. ToxicolPathol2012;40: 971–94.
32.    Muriithi NJ, Maina GS, Maina MB, Kiambi MJ, Kelvin JK, Umar A, John MK, Ann NW, Piero NM, Eliud NNM. Determination of hematological effects of methanolic leaf extract of Vernonialasiopus in normal mice. J Blood Lymph 2015; 5(3): 01-06.
33.    Debelo N, Afework M, Debella A, Makonnen E, Ergete W, Geletaet B. Assessment of hematological, biochemical and histopathological effects of acute and sub-chronic administration of the aqueous leaves extract of Thymus schimperi in rats. J ClinToxicol 2016;6(2): 01-09.
34.    Mehrdad M, Messripour M, Ghobadipour M. The effect of ginger extract on blood urea nitrogen and creatinine in mice. Pak J BiolSci 2007; 10(17): 2968-71.
35.    Pandit A, Sachdeva T, Bafna P. Drug-induced hepatotoxicity: a review. J. Appl. Pharm Sci 2(5): 233–43.
36. Asif M. A brief study of toxic effects of some medicinal herbs on kidney. Adv Biomed Res 2012;1: 44.

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