Author(s): Sambasiva Rao Puram, Nithya G

Email(s): nithyaranju@gmail.com

DOI: 10.5958/0974-360X.2020.01006.9   

Address: Sambasiva Rao Puram1, Dr Nithya G1*
1Department of Chemistry, School of Basic Sciences, VISTAS, Chennai, Tamilnadu, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 12,     Year - 2020


ABSTRACT:
The objective of this work is to determine the plasma concentrations of Moxinidine by liquid chromatography coupled with triple quad mass spectrometer (LC-MS/MS) using internal method. Method development was conducted in such way that sufficient sensitivity achieved and no short term and long term matrix effects effect the method performance. Sensitivity found superior in positive ionization than negative ionization. Sum of multiple daughter ions were used as mass transactions for Moxonidine are 242.05/206.1 and 242.05/199.05 where as single mass transaction was used for Clonidine are 230.1/213.1. Chromatography was optimized using Acetonitrile: Buffer (10mmol Ammonium Acetate) (85:15) on Hypurity C8, 100 x 4.6 mm analytical column. Calibration curve found linear over the range 5.004 to 10345.023 pg/ml using 1/x2 as weighting factor. Method validation was conducted as per international method validation guidelines. The method is successfully applied to analyze 360 clinical study samples collected after administration of 25mg of Moxonidine in a IEC approved two phase clinical study. Incurred sample reanalysis was conducted and found method is reproducible.


Cite this article:
Sambasiva Rao Puram, Nithya G. A Sensitive, Rapid Determination of Moxonidine in Plasma by LC-MS/MS, Application to Bioequivalence Study. Research J. Pharm. and Tech. 2020; 13(12):5773-5776. doi: 10.5958/0974-360X.2020.01006.9


REFERENCES:
1.    http://wikipedia\moxonidine, encyclopedia, 2016 [Accessed: 20 Dec 2019).
2.    http://EMA, Note for guideline on the investigation of BA/BE, 2000 [Accessed: 20 Dec 2019).
3.    http://emc, Moxonidine Specific product characteristics, 2012 [Accessed: 20 Dec 2019).
4.    Minxia M. He, Trent. Abraham, Thomas J. Lindsay, Hans C. Schaefer, Isabelle J. Pouliquen, Chris Payne: The American Society for Pharmacology and Experimental Therapeutics, Vol.31, no.3, 2003, 334-342.
5.    Stephen D Wise, Clark Chan, Hans G Schaefer, Minxia M He, Isabelle J Pouliquen, Malcolm I Mitchell: Br J Clin Pharmacol, 2002, 54(3), 251–254.
6.    Luhua Zhao, Li Ding, Xin Wei: Journal of Pharmaceutical and Biomedical Analysis 2006, 40(1):95-9.
7.    Milovanović S, Otašević B, Zečević M, Zivanović L, Protić A: J Pharm Biomed Anal. 2012, 5(59), 151-6.
8.    Rajendra Kakde, Kamlesh Gadpayale, M. Obaid Qureshi: International Journal of PharmTech Research, Vol.4, No.1, 2012, 358-363.
9.    http://EMA, Moxonidine, public assessment report, 2009 [Accessed: 20 Dec 2019).
10.    CUI, Han-ming, SUN, Hui-min, WANG, Wei, TIAN, Song-jiu: HPLC Determination of Dissolution of Moxonidine Hydrochloride Tablets, Chinese, Journal of Pharmaceutical Analysis, Volume 23, Number 4, July 2003 pp. 315-316(2).
11.    Milovanović S, Otašević B, Zečević M, Zivanović L, Protić A: Development and validation of reversed phase high performance liquid chromatographic method for determination of moxonidine in the presence of its impurities, J Pharm Biomed Anal, Feb 2012, 59:151-6.
12.    Lena Brynne, John L McNay, Hans G Schaefer, Karl Swedberg, Curtis G Wiltse, and Mats O Karlsson: Pharmacodynamic models for the cardiovascular effects of moxonidine in patients with congestive heart failure, Br J Clin Pharmacol, Jan 2001, 51(1): 35–43.
13.    Rudolph M, Janssen W, Strassner M: Determination of moxonidine (BDF 5895) in plasma by gas chromatography-negative ion chemical ionization mass spectrometry, J Pharm Biomed Anal, May 1992,10(5):323-8.
14.    Chazova I, Almazov VA, Shlyakhto E: Moxonidine improves glycaemic control in mildly hypertensive, overweight patients: a comparison with metformin, Diabetes Obes Metab, Jul 2006, 8(4):456-65.
15.    Slavica Filipic, Milica Elek, Marija Popović, Katarina Nikolic, and Danica Agbaba: Development of Hydrophilic Interaction Liquid Chromatography Method for the Analysis of Moxonidine and Its Impurities, Journal of Analytical Methods in Chemistry, Volume 2016 (2016).

Recomonded Articles:

Author(s): Nimbalkar V.V., Pansare P.M., Nishane B.B.

DOI: 10.5958/0974-360X.2015.00310.8         Access: Open Access Read More

Author(s): Muhammad Hamdan, Noorhamdani AS, Masruroh Rahayu, Mohammmad Hasan Machfoed

DOI: 10.5958/0974-360X.2019.01020.5         Access: Open Access Read More

Author(s): Trophimus Gnanabagyan Jayakaran, Vignesh R, Shankar P

DOI: 10.5958/0974-360X.2019.00700.5         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Raj Kumar R, Praveen D, Palani Shanmugasundaram

DOI: 10.5958/0974-360X.2016.00164.5         Access: Open Access Read More

Author(s): Vaseeha Banu T.S., Sandhya K.V., K.N. Jayaveera

DOI: Not Available         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Siripurapu Sriram, Venkata Ravibabu Mandla, Chaithanya Sudha M, Saladi S.V. Subba Rao, Nagaveni Ch, Vani V., Gobinath P., Raj Kumar KVG., Jaydeep Lella, Sannidhi Krishna Praveen, Debjit Datta

DOI: 10.5958/0974-360X.2017.00078.6         Access: Open Access Read More

Author(s): Archana Kushwaha, Jayanti Jaiswal, Priya Singh, Neha Rathore, Jai Prakash Dhruw, Ishu Sahu, Chhaya Singh, Krinsha Kumar Sahu, Mukta Agrawal, D. K. Tripathi, Ajazuddin, Amit Alexander

DOI: 10.5958/0974-360X.2017.00270.0         Access: Open Access Read More

Author(s): M. Sumithra, Avantika Prabhabanik

DOI: 10.5958/0974-360X.2017.00036.1         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Barokar A.A., Wagh R.D., Baviskar D.T., Shaikh T.J.

DOI: Not Available         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags