Seema B Chauhan, Vinod Singh, Rajani Chauhan
Seema B Chauhan1, Vinod Singh2, Rajani Chauhan3*
1,3Department of Pharmaceutical Sciences, Banasthali Vidyapith, Vanasthali, Rajasthan, India.
2Department of Pharmaceutical Sciences, Gurukul Kangri University, Haridwar, Uttarakhand, India.
Volume - 13,
Issue - 12,
Year - 2020
Probiotics are the beneficial microorganism possesses many health benefits for human being upon their use. The major limitation in the use of probiotics is their loss of viability during storage as well during passage through the upper gastrointestinal tract. Probiotic encapsulation technology has the potential to protect microorganisms and to deliver them into the gut. In order to overcome the limitation of viability in upper gastrointestinal tract, Lactobacillus Rhamnosus GG encapsulated pectin and sodium carboxy methyl cellulose microbeads of probiotics: polymer ratio (1:1, 1:2 and 1:3) were fabricated using the ionotropic gelation technique. In addition, the developed microbeads were coated with Eudragit S 100 and cellulose acetate phthalate and assessed in vivo anti-diarrheal activity and compared with free probiotics. The developed microbeads have shown increase of probiotics: polymer ratio significantly improves viability of probiotics and therefore shown quick onset of action with encapsulated microbeads as compared to free probiotics. Thus, coated microbeads could be a potential delivery system for treatment of antibiotic associated diarrhea.
Cite this article:
Seema B Chauhan, Vinod Singh, Rajani Chauhan. Effect of Encapsulated Lactobacillus rhamnosus GG in Antibiotic associated diarrhea in Albino Wistar rat model. Research J. Pharm. and Tech. 2020; 13(12):5736-5742. doi: 10.5958/0974-360X.2020.00999.3
1. Wistrom J, Norrby SR, Myhre EB, Eriksson S, Granstrom G, Lagergren L, Englund G, Nord CE, Svenungsson B. Frequency of antibiotic-associated diarrhoea in 2462 antibiotic-treated hospitalized patients: a prospective study. J Antimicrob Chemother. 2001; 47(1): 43-50.
2. Barbut F, Meynard JL, Guiguet M, Avesani V, Bochet MV, Meyohas MC, Delmée M, Tilleul P, Frottier J, Petit JC. Clostridium difficile-associated diarrhea in HIV-infected patients: epidemiology and risk factors. J Acquir Immune Defic Syndr Hum Retrovirol. 1997; 16(3): 176-181.
3. McFarland LV, Surawicz CM, Stamm WE. Risk factors for Clostridium difficile carriage and C. difficile-associated diarrhea in a cohort of hospitalized patients. J Infect Dis. 1990; 162(3): 678-684.
4. Bignardi GE. Risk factors for Clostridium difficile infection.J Hosp Infect. 1998; 40(1): 1-15.
5. Marteau PR, de Vrese M, Cellier CJ, Schrezenmeir. Protection from gastrointestinal diseases with the use of probiotics. J Am J Clin Nutr. 2001 Feb; 73(2 Suppl): 430S-436S.
6. Lokhande S, More S, Raje V. A Systematic Study of Probiotics- An Update Review. Asian J. Pharm. Tech. 2018; 8 (3):149-157.
7. Swathi KV. Probiotics - A Human Friendly Bacteria. Research J. Pharm. and Tech 2016; 9(8): 1260-1262.
8. Devi NKD, Sujitha K, Madhavi BR, Mrudula BS, Janaki MN, Ramya VS. Symbiosis - A Friendly Relationship between Man and Microbes. Research J. Science and Tech. 2010; 2 (1): 1-7.
9. NKD Devi, K Sujitha, BR Madhavi, BS Mrudula, MN Janaki, V Sri Ramya. Symbiosis – A Friendly Relationship between Man and Microbes. Research J. Science and Tech. 2010; 2(1): 1-7.
10. Thantsha M, Mamvura C, and Booyens J. Probiotics-what they are, their benefits and challenges. In: Brzozowski, T, editors. New advances in the basic and clinical gastroenterology, Croatia: InTech Publisher 2012, p. 22-50.
11. Rodklongtan A, La‐ongkham O, Nitisinprasert S, Chitprasert P. Enhancement of Lactobacillus reuteri KUB‐AC5 survival in broiler gastrointestinal tract by microencapsulation with alginate-chitosan semi‐interpenetrating polymer networks. J Appl Microbiol 2014; 117(1): 227-238.
12. Gambhire MS, Lilke S, Gambhire VM. Microencapsulation- An Overeview. Research J. Pharma. Dosage Forms and Tech. 2010; 2(4): 270-276.
13. Akel H, Madani F, Ibrahim W. Preparation of Paracetamol Microcapsules by Complex Coacervation and Studying the effect of some Factors Influencing Microencapsulation Yield and Efficacy. Research J. Pharm. and Tech 2017; 10(10): 3271-3275.
14. Salve PS. Development of Controlled Release Floating Beads of Ibuprofen using Ionotropic Gelation Technique. Research J. Pharma. Dosage Forms and Tech. 2011; 3(6): 260-268.
15. Mali SD, Khochage SR, Nitalikar MM, Magdum CS. Microencapsulation: A Review. Research J. Pharm. and Tech. 6(9): 2013; 954-961.
16. Willliams RO, and Liu J. Influence of processing and curing conditions on beads coated with an aqueous dispersion of cellulose acetate phthalate. Eur J Pharm Biopharm 2000; 49(3): 243-252.
17. Arica B, Arica MY, Kas HS, Hincal AA, Hasirci V. In vitro studies of enteric coated Diclofenac sodium-carboxymethylcellulose microspheres. J Microencapsul 1996; 13(6): 689-699.
18. U. D. Shivhare, G. S. Chhabra, V. B. Mathur, S. B. Patel. Microencapsulation of Acyclovir into Eudragit S100 Using Emulsion-Solvent Evaporation Method. Research J. Pharm. and Tech. 4(4): April 2011; Page 652-658.
19. Jawed Akhtar, Saurabh Kanoongo, Deepak K. Mittal, Rajesh Sharma, Vaibhav Solanki. The New Developed Concepts and Trends in Microencapsulation. Research J. Pharma. Dosage Forms and Tech. 2011; 3(3): 75-83 .
20. Fiol, F.D.S.D., Tardelli, A.C.M., Marciano, J.J., Marques, M.C., Sant'Ana, L.L. Obesity and the Use of Antibiotics and Probiotics in Rats. Chemotherapy. 2014; 60: 162-167.