Author(s): Bonagiri Sreedevi, Vijaya Kuchana, S. Shobharani

Email(s): sreedevi.bonagiri@gmail.com

DOI: 10.5958/0974-360X.2020.01087.2   

Address: Bonagiri Sreedevi1*, Vijaya Kuchana2, S. Shobharani3
1Department of Pharmaceutical Chemistry, T.R.R College of Pharmacy, Meer Pet, Hyderabad.
2Department of Pharmaceutical Chemistry, Teegala Krishna Reddy College of Pharmacy, Hyderabad.
3Department of Pharmaceutical Chemistry, Center for Pharmaceutical Sciences, Institute of Sciences and Technology, JNTU-H. Hyderabad, Telangana, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 12,     Year - 2020


ABSTRACT:
Diabetes mellitus is a metabolic disorder caused due to insufficient insulin or insulin resistance in our body. Diabetes mellitus is of three types. They are type I, type II and gestational diabetes mellitus. Diabetes mellitus can be diagnosed by monitoring blood glucose levels and HbA1c. It has common clinical presentations like polyuria, polyphagia, weight gain or weight loss, dehydration, fainting etc,. If left untreated diabetes can lead to life threatening complications. Some of the complications are Diabetic keto acidosis, Diabetic nephropathy, Alzhimer’s disease, Diabetic foot, chronic kidney disease, stroke, etc,. Insulin is used for the treatment of type I diabetes and type II diabetes can be treated using some anti-diabetic drugs along with few precautionary measures and life style modifications. Holarrhena pubescensis a medicinal plant commonly called kurchi. All parts of this plant has got some medicinal use and as a whole, the plant has wide range of medicinal benefits. The chloroform and ethanolic extracts of Holarrhena pubescens showed significant results when compared to the standard drug Metformin. Cell lines showed 50% cell death when 1µg/ml of Metformin was given. The same result was obtained when 100µg/ml of H.P.C.E. and 100µg/ml H.P.E.E. was given. The glucose uptake assay for H.P.C.E. at a concentration of 10µg/ml was 5.85±0.02mg/100ml in the absence of insulin and 7.89±0.05 mg/100ml in the presence of insulin and for H.P.E.E. at a concentration of 10µg/ml was 9.45±0.05mg/100ml in the absence of insulin and 10.54±0.04mg/100ml in the presence of insulin. Inhibition of ??-amylase enzyme activity ??-glucosidase enzyme activity of H.P.C.E. and H.P.E.E. were compared with standard and the results are discussed below. This article reveals that Holarrhena pubescens has significant anti-diabetic property. Its folklore usage in treating diabetic condition has been validated with this scientific result.


Cite this article:
Bonagiri Sreedevi, Vijaya Kuchana, S. Shobharani. Estimation of Anti-Diabetic property of Holarrhena pubescens by In Vitro method using L6 rat Skeletal Muscle Cell Lines. Research J. Pharm. and Tech. 2020; 13(12):6238-6242. doi: 10.5958/0974-360X.2020.01087.2


REFERENCES:
1.    Rambabu B, Rao Patnaik KSK. Antidiabetic and antiulcer activity of ethanolic flower extract of Jasminum sambac in rats. Asian Journal of Research in Chemistry. 2014;7(6):580-585.
2.    Satyaseelan Thennarasan, Subbiah Murugesan, Vajiravelu Sivamurugan. Antidiabetic efficacy of methanol extract of Brown alga Lobophora variegate on Alloxan stimulated hyperglycaemic Wistar albino rats. Asian Journal of Pharmacy and Technology,2017;7(3):157-165.
3.    Pawar MP, Patil NP, Baviskar DT. Antidiabetic uses of some common herbs in postal region of Dhule district of Maharashtra. Research Journal of Pharmaceutical Dosage Forms and Technology,2013;5(2):62-64.
4.    American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2014;37 Suppl 1: S81–S90.
5.    Galtier F. Definition, epidemiology, risk factors. Diabetes Metab. 2010; 36:628–651
6.    Abbas E. Kitabchi, Guillermo E. Umpierrez, John M. Miles, Joseph N. Fisher. Hyperglycemic crisis in adult patients with diabetes. Diabetes Care. 2009 Jul;32(7):1335-1343.
7.    Sathya Krishnasamy, Thomas L. Abell. Diabetic gastroparesis: Principles and current trends in management. Diabetes Ther. 2018 Jul; 9 (suppl 1):1-42
8.    Thunander M, Törn C, Petersson C, Ossiansson B, Fornander J, Landin-Olsson M. Levels of C-peptide, body mass index and age, and their usefulness in classification of diabetes in relation to autoimmunity, in adults with newly diagnosed diabetes in Kronoberg, Sweden. Eur J Endocrinol. 2012;166: 1021–1029.
9.    9.Stone MA, Camosso-Stefinovic J, Wilkinson J, de Lusignan S, Hattersley AT, Khunti K. Incorrect and incomplete coding and classification of diabetes: a systemic review. Diabet Med.2010;27:491–497
10.    Daneman D. Type 1 diabetes. Lancet. 2006; 367:847–858.
11.    Devendra D, Liu E, Eisenbarth GS. Type 1 diabetes: recent developments. BMJ. 2004; 328:750–754.
12.    International Diabetes Federation. IDF Diabetes Atlas. 6th ed. Brussels, Belgium: International Diabetes Federation; 2013
13.    Chiang JL, Kirkman MS, Laffel LM, Peters AL; Type 1 Diabetes Sourcebook Authors. Type 1 diabetes through the life span: a position statement of the American Diabetes Association. Diabetes Care. 2014; 37:2034–2054.
14.    Pickup JC. Insulin-pump therapy for type 1 diabetes meliitus. N Engl J Med.2012; 366:1616-24.
15.    Yeh HC, Brown TT, Maruthur N, et al. Comparative effectiveness and safety of methods of insulin delivery and glucose monitoring for diabetes mellitus: a systemic review and meta analysis. Ann Intern Med. 2012;157:366-47.
16.    Shika Batra, Nikhil Batra, Anil Pareek, Badri Prakash Nagori. Invitro study of Cayratia trifolia (L.) Domin on isolated rat hemi-diaphragm by glucose uptake method. Research Journal of Pharmacy and Technology,2012;5(5):691-693.
17.    Halban PA, Polonsky KS, Bowden DW, Hawkins MA, Ling C, Mather KJ, Powers AC, Rhodes CJ, Sussel L, Weir GC. Β-cell failure in type II diabetes: postulated mechanisms and prospects foe prevention and treatment. Diabetes Care.2014;37:1751-1758
18.    Reinehr T. Type 2 diabetes mellitus in children and adolescents. World J Diabetes.2013;4:270-281
19.    Sujatha kumara M, Kiran babu M, Md Rehana sultana, Srinivas M, Prasanthi Ch. Diabetes Mellitus: Present status and drug therapy updates. Research Journal of Pharmacy and Technology,2014;7(1):84-94.
20.    Juan José Marín- Peñalver, Iciar Martín-Timón, Cristina Sevillano-Collantes, Francisco Javier del Cañizo-Gómez. Update on the treatment of type 2 diabetes mellitus. World J Diabetes.2016 sep 15;7(17):354-395.
21.    “Department of AYUSH”. Department of AYUSH. Ministry of Health and Family Welfare. Government of India.
22.    India Biodiversity Portal. Holarrhena pubescens Wall. Ex G Don.
23.    Sarot Cheenparacha, Pannnakorn Boapun, Thunwadee Limtharakul, Stephen G Pyne. Antimalarial and cytotoxic activities of pregnene-type steroidal alkaloids from Holarrhena pubescens roots. Natural Product Research. 2019;33(6):782-788.
24.    Panda SK, Patra N, Sahoo G, Bastia AK, Dutta SK. Antidiarrhoeal activities of medicinal plants of similipal biosphere reserve, Odisha, India. Int J Med Arom Plants.2012;2(1):123-134.
25.    Dey A, De JN. Ethnobotanical survey of Purulia district, West Bengal, India, for medicinal plants used against gastrointestinal disorders. J Ethnopharmacol.2012;143:68-80.
26.    Patil R, Devkar S, Pawar P, Pattewar A. Invitro anthelminthic activity of Holarrhena antidysenterica bark. Int Pharma Res Dev.2012;4(3):147-150.
27.    Shahabuddin KU, Sarwar MS, Mohiuddin E. Clinical evaluation of some herbal medicine for amoebiasis. Pak J Pharmacol.2006;23(2):9-12.
28.    Anup Bhusal, Nirmala Jamarkattel, Aasmin Shrestha, et al. Evaluation of antioxidative and antidiabetic activity of bark of Holarrhena pubescens wall. J Clin Diag Res.2014 sep; 8(9): HC05-HC08
29.    Daniel M. Medicinal plants: chemistry and properties. Science, Enfield, NH 2006.
30.    Sugumaran M, Vetrichelvan T, Darlin Quine S. Antidiabetic potential of aqueous and alcoholic leaf extracts of Pithecellobium dulce. Asian Journal of Research in Chemistry,2009;2(1):83-85.
31.    Subramaniam Ramachandran, Aiyalu Rajasekaran, Natarajan Adhirajan. In Vivo and In Vitro antidiabetic activity of Terminalia paniculata bark: An evaluation of possible phytoconstituents and mechanisms for blood glucose control in diabetes. ISRN Pharmacology.2013; Article Id:484675.
32.    Wu.H.-S, Zhu. D.-F, Zhou C.-X et al. Insulin sensitizing activity of ethyl acetate fraction of Acorus calamus L. In Vitro and In Vivo. Journal of Ethnopharmacology.2009;123(2):288-292.
33.    Edwin Jose B, Dr. Paneerselvam P. Invitro anti diabetic activity of ethanol and aqueous leaf extracts of Azima tetracantha Lam. Research Journal of Science and Technology, 2019;11(1): 64-68.
34.    Kuppusamy A, Muthusamy U, Andichetiar Thirumalaisamy S, Varadharajan S, Ramasamy K, Ramanathan S. Invitro (𝛼glucosidase and 𝛼-amylase inhibition) and invivo antidiabetic property of phytic acid (IP6) in streptozotocin-nicotinamide induced type 2 diabetes mellitus (NIDDM) in rats. Journal of Complementary and Integrative Medicine.2011;8(9): no.1.
35.    Lelliott C, Vidal-Puig AJ. Lipotoxicity, an imbalance between lipogenesis de novo and fatty acid oxidation. International Journal of obesity and related metabolic disorders.2004;28:22-28.
36.    Ranadheer Chowdary P, Praveen D, Vijey Aanandhi M. A prospective study on incidence of Dyslipidemia in diabetes mellitus. Research Journal of Pharmacy and Technology, 2017;10(2):431-433.
37.    Parisa Parsa, Roya Ahmadinia-Tabesh, Younes Mohammadi. Assessment of the risk of Coronary Heart disease in diabetes patients type II. Asian Journal of Nursing education and Research,2019;9(2):267-270.
38.    Hepcy Kalarani D, Venkatesh P, Dinakar A. Antidiabetic activity of aqueous extract of leaves of Pavonia zeylanica in rats. Research Journal of Pharmacy and Technology, 2009;2(4):789-792.

Recomonded Articles:

Author(s): Nimbalkar V.V., Pansare P.M., Nishane B.B.

DOI: 10.5958/0974-360X.2015.00310.8         Access: Open Access Read More

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): Koushika Das, Pranit Krishna, Avipsha Sarkar, Shanmuga Sundari Ilangovan, Shampa Sen

DOI: 10.5958/0974-360X.2017.00267.0         Access: Open Access Read More

Author(s): U.S Mahadeva Rao, Khamsah Suryati Mohd, Abdurrazaq Muhammad, Bashir Ado Ahmad, Mohaslinda Mohamad, Rosmawati Mat Ali

DOI: Not Available         Access: Open Access Read More

Author(s): Sandeep D. S, Prashant Nayak, Jobin Jose, Rishal Relita M, Sumana D. R.

DOI: 10.5958/0974-360X.2017.00317.1         Access: Open Access Read More

Author(s): Ankit Patel, Pankaj Kushwah, Sujit Pillai, Ajay Raghuvanshi, Nitin Deshmukh

DOI: 10.5958/0974-360X.2017.00013.0         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Deepak Karki, Gururaj S. Kulkarni, Shivakumar Swamy, Sheeba FR

DOI: 10.5958/0974-360X.2017.00750.8         Access: Open Access Read More

Author(s): Praveen D, Ranadheer Chowdary P, Gandikota Thanmayi, Gangabathina Poojitha, Vijey Aanandhi M

DOI: 0.5958/0974-360X.2016.00047.0         Access: Open Access Read More

Author(s): Pankaj Kumar, Jane Jacob, Khadjeeth Ajina, Jazeela, Fathima

DOI: 10.5958/0974-360X.2016.00101.3         Access: Open Access Read More

Author(s): Dhanalekshmi UnniKrishnan Nair, MP Saraswathy, Narra Kishore, Neelakanta Reddy Pully

DOI: Not Available         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Malarkodi Velraj, P. Jasmine Shiney, Biplab Paul, Rashmi. S, Nivethitha

DOI: 10.5958/0974-360X.2017.00006.3         Access: Open Access Read More

Author(s): B. Thawkar, M. Kale, M. Oswal, K. Maniyar, K. Kadam, S. Kamat

DOI: 10.5958/0974-360X.2016.00044.5         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags