The bacterium Pseudomonas aeruginosa is a chief causative agent of hospital infections, and contamination with antibiotic-resistant strains may be dangerous. A total of 35 wound burn specimens were collected from patients, and 15 isolates were recognized as P. aeruginosa by microbial cultures and VITIK. Bacterial genomic DNA was extracted, and the presence of the tox A gene was detected by PCR by using specific primers. The results revealed that toxA gene was prevalent in all studied local strains (100 %) from burn wound infections. In the current study most of the mutations in toxA sequence were trans version and transition. Studying and understanding of virulence genes in P. aeruginosa may facilitate the development of novel vaccines and drug treatments in the future.
Cite this article:
Shurooq Rayyis Kadhim. Genetic Study of Txo A Gene in Pseudomonas aeruginosa Isolated from Burn Wound Infections. Research J. Pharm. and Tech. 2020; 13(11):5301-5305. doi: 10.5958/0974-360X.2020.00927.0
1. Hirsch EB, Tam VH, Impact of multi - drug resistant Pseudomonas aeruginosa infection on patient outcomes. Expert Rev. Pharmacoecon. Outcomes Res. 2010; 10: 441–451. (doi:10.1586/erp.10.49).
2. Moore NM, Flaws ML, Epidemiology and pathogenesis of Pseudomonas aeruginosa infections. Clin. Lab. Sci. 2011; 24: 43–46.
3. Sedlak-W, Cripps J, Kyd A, Fox w, Pseudomonas aeruginosa : the potential to immunize against infection, Expert Opin. Bio. Ther.2005;5: 967-982.
4. Faezi S, Safarloo M, Amir mozafari N, et al. Protective efficacy of Pseudomonas aeruginosa type-A flagellin in the murine burn wound model of infection. APMIS. 2014; 122: 115-27.
5. Rumbaugh KP, Hamood AN, Griswold JA, Analysis of Pseudomonas aeruginosa clinical isolates for possible variations within the virulence genes exotoxin A and exoenzyme S.J. Surg. Res. 1999; 82: 95–105.
6. Lee VT, Smith RS, Tummler B, et al., Activities of Pseudomonas aeruginosa effectors secreted by the Type III secretion system in vitro and during infection. Infect Immun. 2005;73: 1695–705.
7. Dong D, Zou D, Liu H, Yang Z, et al., Rapid detection of Pseudomonas aeruginosa targeting the toxA gene in intensive care unit patients from Beijing, China. Front. Microbiol. 2015; 6: 1100.
8. 8.Collee JG, Marmion BP, Fraser AG, et al. Mackie and McCartney practical medical microbiology. London: Churchill Livingstone.1996; 14th ed: pp.14.
9. Sambrook, J, and Rusell, D. W, Molecular cloning a laboratory manual. Cold spring Harbor, NY: cold spring Harbor Laboratory press. 2001.
10. Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000; 31:959-64.
11. Neamah AA, Molecular Detection of virulence factor genes in Pseudomonas aeruginosa isolated from human and animals in Diwaniya province. Kufa J. Vet. Med. Sic. 2017; 8: 218.
12. Raziq AH, Molecular Study of Two Virulence Genes of Pseudomonas aeruginosa, The Oxa 10 and Tox A with The Comparisons of The Relevant Sequences. Med. J. Babyl. 2017; 14:28-38.
13. Michalska M and Wolf P. Pseudomonas Exotoxin A: optimized by evolution for effective killing. Front Microbiol. 2015,15;6:963.
14. Sokurenko, E.V. et al., Pathoadaptive mutations: gene loss and variation in bacterial pathogens. 1999.
15. Eman T, and Sawsan S, Genetic detection of some virulence genes in Pseudomonase aeruginosa isolated from cystic fibrosis and no-cystic fibrosis patients in Iraq. J. Genet. Environ. Resour. Conserv., 2014, 2;3:380-387.
16. Rana M, Abbas F, DNA Sequences Analysis of tox A Gene Isolated from Pseudomonas aeruginosa Bacteria. J.Biotech.Res.Cen.2016;10 :30-41.
17. Mona A, Mona S, and Nadia M, Genetic Identification of Pseudomonas aeruginosa Virulence Genes among Different Isolates, J. Microb. Biochem. Technol. 2015, 7:5.