S. Veena, P. Keerthana, A. Jayabalaji, M. Yasasve, L. A. Vishal
S. Veena1, P. Keerthana2, A. Jayabalaji2, M. Yasasve2, L. A. Vishal2*
1Centre for Nano Science and Technology, A. C. Tech Campus, Anna University, Chennai, Tamil Nadu, India.
2Department of Biotechnology, Sree Sastha Institute of Engineering and Technology (Affiliated to Anna University), Chennai, Tamil Nadu, India.
Volume - 13,
Issue - 11,
Year - 2020
Aeromonas species are common contaminants of fish and seafood. This study presents key findings of the Aeromonas species that were isolated from the organs of fishes collected from Kasimedu region in Chennai, Tamil Nadu, India. In this study, bacterial isolates from intestines and gills of three different edible fish varieties - Sardinella longiceps, Lutjanus gibbus and Pseudocaranx dentex were isolated. The isolates were identified to belong to gram negative Aeromonas species through biochemical tests. All the isolates were tested for antibiotic sensitivity for ten different antibiotics which includes Ampicillin, Chloramphenicol, Erythromycin, Gentamycin, Penicillin-G, Streptomycin, Tetracycline, Fluoroquinolone, Cephalosporin and Carbapenam. Metal resistance was checked for five metals namely Copper (Cu), Chromium (Cr), Lead (Pb), Manganese (Mn) and Nickel (Ni). All the strains exhibited Multiple Antibiotic Resistances (MAR) to at least two antibiotics. The Aeromonas strains showed sensitivity to Erythromycin and Gentamycin. The MAR index for the three strains was calculated as 0.52, 0.47 and 0.28 respectively. All the three MAR indices values were seen to be greater than 0.2 which indicates that the isolate has originated from a high-risk source of contamination. Thus, the results obtained clearly show that the fish samples collected is highly contaminated with antibiotic and metal resistant Aeromonas bacterial species.
Cite this article:
S. Veena, P. Keerthana, A. Jayabalaji, M. Yasasve, L. A. Vishal. Antibiotic and Metal Resistance offered by Aeromonas species isolated from Edible Fishes in Chennai, Tamil Nadu: A Field Investigation. Research J. Pharm. and Tech. 2020; 13(11):5197-5201. doi: 10.5958/0974-360X.2020.00908.7
1. Vijayakumar S, Vaseeharan B, Malaikozhundan B, Gobi N, Ravichandran S, Karthi S, Ashokkumar B and Sivakumar N. A novel antimicrobial therapy for the control of Aeromonas hydrophila infection in aquaculture using marine polysaccharide coated gold nanoparticle. Microb. Pathog. 2018; 110: 140-151.
2. Ahmad M. Mercuric resistant bacteria Aeromonas exhibits neurologic toxic effects on the developmental motor reflexes, and brain oxidative stress in mice offspring. Microb. Pathog. 2018; 114: 169-175.
3. Ramesh D and Souissi S. Antibiotic resistance and virulence traits of bacterial pathogens from infected freshwater fish, Labeo rohita. Microb. Pathog. 2018; 116: 113-119.
4. Cabello FC. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ. Microbiol. 2006; 8: 1137-1144.
5. Alikunhi NM, Batang ZB, Al-Jahdali HA, Aziz MA and Al-Suwailem AM. Culture-dependent bacteria in commercial fishes: Qualitative assessment and molecular identification using 16S rRNA gene sequencing. Saudi J. Biol. Sci. 2016; 24: 1105-1116.
6. Papadopoulou C, Economou E, Zakas G, Salamoura C, Dontorou C and Apostolou J. Microbiological and Pathogenic Contaminants of Seafood In Greece. J. Food Quality. 2004; 30: 28-42.
7. Tokajian S and Hashwa F. Phenotypic and genotypic identification of Aeromonas spp. isolated from a chlorinated intermittent water distribution system in Lebanon. J. Water Health. 2004; 2: 115-122.
8. Janda JM and Abbott SL. The genus Aeromonas: Taxonomy, pathogenicity, and infection. Clin. Microbiol. Rev. 2010; 23: 35-73.
9. Slade PJ, Falah MA and AI-Ghady MR. Isolation of Aeromonas hydrophila from bottled waters and domestic water supplies in Saudi Arabia. J. Food Prot. 1986; 49: 471-476.
10. Taher AAI, Rao BN, Alganay KG and El-Arabi MB. An outbreak of acute gastroenteritis due to Aeromonas sobria in Benghazi, Libyan Arab Jamahiriya. East. Mediterr. Health J. 2000; 6: 497-499.
11. Wilson JW, Schurr MJ, LeBlanc CL, Ramamurthy R, Buchanan KL and Nickerson CA. Mechanisms of bacterial pathogenicity. Postgrad. Med. J. 2002; 78: 216-224.
12. Watson IM, Robinson JO, Burke V and Gracey M. lnvasiveness of Aeromonas spp. in relation to biotype, virulence factors and clinical features. J. Clin. Microbiol. 1985; 22: 48-51.
13. Sauer S, Freiwald A, Maier T, Kube M, Richard R, Markus K and Klaus G. Classification and identification of bacteria by mass spectrometry and computational analysis. PLoS One. 2008; 3: e2843.
14. Bauer AW, Kirby WM, Sherris JC and Turck M. Antibiotic Susceptibility Testing by a Standardized Single Disk Method. Am. J. Clin. Pathol. 1966; 45: 493-496.
15. Sandhu R, Dahiya S and Sayal P. Evaluation of multiple antibiotic resistance (MAR) index and Doxycycline susceptibility of Acinetobacter species among inpatients. Indian J. Microbiol. Res. 2016; 3: 299-304.
16. Wiegand I, Hilpert K and Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat. Protoc. 2008; 3: 163-175
17. Penderup Jensen M, Braun A, Vogensen FK and Ardö Y. Study of antimicrobial activity among Lactobacillus helveticus strains using three different assays. Ann. Microbiol. 2009; 59: 187-190.
18. Shabhana S, Karthika M, Suganya P, Ramasubramanium V. Antibiogram of Pathogenic bacteria isolated and identified from edible fish Labeo rohita. Int. J. Biosci. 2018; 12: 42-50.
19. Figueira V, Vaz-Moreira I and Silva M. Diversity and antibiotic resistance of Aeromonas spp. in drinking and waste water treatment plants. Water Res. 2011; 45: 5599-5611.
20. Evangelista-Barreto NS, de Carvalho FC, Vieira RH, Dos Reis CM, Macrae A and Rodrigues Ddos P. Characterization of Aeromonas species isolated from an estuarine environment. Braz. J. Microbiol. 2010; 41: 452-460.
21. Al-shalah LAM. The study characters of Aeromonas hydrophila on some media. Mesop. Environ. J. 2017; Special Issue A: 21-129.
22. Resende JA, Silva VL, Fontes CO, Souza-Filho JA, Oliveira TLR, Coelho CM, Cesar DE and Diniz CG. Multidrug-resistance and toxic metal tolerance of medically important bacteria isolated from an aquaculture system. Microbes Environ. 2012; 27: 449-455.
23. Devi SM, Balachandar V, Lee SI and Kim IH. An Outline of Meat Consumption in the Indian Population - A Pilot Review. Korean J. Food Sci. Anim. Resour. 2014; 34: 507-515.
24. Majumdar T, Ghosh S, Pal J and Mazumder S. Possible role of a plasmid in the pathogenesis of a fish disease caused by Aeromonas hydrophila. Aquaculture. 2006; 256: 95-104.
25. Chaminuka N and Dube E. Urban Agriculture As A Food Security Strategy For Urban Dwellers: A Case Study Of Mkoba Residents In The City Of Gweru, Zimbabwe. PEOPLE: Int. J. Soc. Sci. 2017; 3: 26-45.
26. Ashiru AW, Uaboi PO and Idika CN. Isolation and antibiotic profiles of Aeromonas species from Tilapia and Catfish. Pak. J. Nutr. 2011; 10; 982-986.
27. Muthu R. Modulation of Immune Response in Edible Fish against Aeromonas hydrophila. J. Clin. Microbiol. Biochem. Tech. 2018; 4: 1-5.
28. Berg J, Tom-Petersen A and Nybroe O. Copper amendment of agricultural soil selects for bacterial antibiotic resistance in the field. Lett. Appl. Microbiol. 2005; 40: 146-151.
29. Wales A and Davies R. Co-selection of resistance to antibiotics, biocides and heavy metals, and its relevance to foodborne pathogens. Antibiotics. 2015; 4: 567-604.