Author(s): Ramu Singh, Pushpa P. Gupta, Amit Roy, Shashikant Chandrakar

Email(s): pushpaprasad81@gmail.com

DOI: 10.5958/0974-360X.2020.00869.0   

Address: Ramu Singh, Dr. Pushpa P. Gupta*, Dr. Amit Roy, Dr. Shashikant Chandrakar
Columbia Institute of Pharmacy, Raipur (C.G.).
*Corresponding Author

Published In:   Volume - 13,      Issue - 10,     Year - 2020


ABSTRACT:
Herbal formulations are traditionally used in Chhattisgarh, India for the management of diverse diseases along with hyperglycemia i.e. diabetes. The healers of different region use their own formulations claiming to treat the disease but their claims have not being properly investigated and verified scientifically. We therefore collected formulations from a healer of Hathbandh district Baloda Bazaar. We prepared hydroalcoholic extract (70:30, HAE) of a formulation and used it for our study. At first acute oral toxicity study was performed to determine the safety of the formulation, thereafter antidiabetic study was performed on streptozotocin (STZ) induced diabetes in wistar rats. The mean blood glucose levels were examined on 0th, 7th, 14th and 28th day in normal, untreated diabetic, diabetic treated with standard and test groups. Data was statistically analyzed by anova software SPSS 14.0. P-value < 0.05 was treated as statistically significant. Toxicity studies showed that extract is not toxic and no behavioral change was observed at a dose of 2000mg/kg body wt. The HAE was administered in 200mg/kg and 400 mg/kg body weight for our studies. The extract at both the doses reduced elevated blood glucose level when compared to diabetic control group. It was also noted that in extract treated group body weight increased in comparison to diabetic control group. HAE at dose of 400 mg/kg body weight showed highest reduction in blood glucose level. The biochemical studies of HAE also supported the claim of traditional healers of Chhattisgarh.


Cite this article:
Ramu Singh, Pushpa P. Gupta, Amit Roy, Shashikant Chandrakar. Evaluation of In vivo Antidiabetic activity of extract of herbal formulation obtained from Hathbandh dist. Baloda Bazaar on streptozotozcin-induced diabetic rats. Research J. Pharm. and Tech. 2020; 13(10):4957-4960. doi: 10.5958/0974-360X.2020.00869.0


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