Author(s): Nugraha Wahyu Cahyana, Edi Widjajanto, Umi Kalsum, Seskoati Prayitnaningsih

Email(s): nugraha.nwc@gmail.com

DOI: 10.5958/0974-360X.2020.00846.X   

Address: Nugraha Wahyu Cahyana1,2*, Edi Widjajanto3, Umi Kalsum4, Seskoati Prayitnaningsih5
1Doctoral Program of Medical Science, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia.
2Department of Ophthalmology, Faculty of Medicine, Jember University, Jember, East Java, Indonesia.
3Department of Clinical Pathology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia.
4Doctoral Program of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia.
5Department of Ophthalmology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia.
*Corresponding Author

Published In:   Volume - 13,      Issue - 10,     Year - 2020


ABSTRACT:
The purpose of this research is to investigate the effect of catechin Isolate from GMB4 Clone Green Tea on apoptotic cell death and oxidative stress in the lens of rats with cataract. Cataract Isolate from GMB4 Clone Green Tea was induced by intraperitoneal injection of 19µmol/kg sodium selenite to ten day-old Wistar rats. The neonatal rats were randomly divided into five groups (n=5 in each group): a control group, and four cataract-induction groups, treated with either 0, 50, 100, 200mg/kg catechin Isolate from GMB4 Clone Green Tea. We performed slit-lamp bio microscopic analysis, level of GSH and GR, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay and immunohistochemistry for caspase-3. Both eyes of all rats in Group 1 did not exhibit cataract formation. In Group 2, one out of five (20%) developed grade 3 cataracts and the remaining four out of five (80%) developed grade 4 cataracts. The di?erence in exhibited cataract in the lens of all rats between Group 2 and any eyes of groups 3 or 4 and 5 were significant (P = 0.022, 0,001, 0,001). The grade of cataract formation was decrease in group 3, 4 and 5. The mean GSH and Caspase-3 levels lenses of group II rats were significantly (P < 0.01) lower than the levels in Group I, Group III, Group IV and Group V. Apoptotic cell death and oxidative stress in the lens that increased following cataract formation in rats was suppressed by cathechin Isolate From GMB4 Clone Green Tea.


Cite this article:
Nugraha Wahyu Cahyana, Edi Widjajanto, Umi Kalsum, Seskoati Prayitnaningsih. Effect of Catechin Isolate from GMB4 Clone Green Tea on Oxidative Stress and Apoptosis in Experimental Cataract. Research J. Pharm. and Tech. 2020; 13(10):4811-4816. doi: 10.5958/0974-360X.2020.00846.X


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