Author(s):
Nayak Apoorva, Rao Rashmi R, Shenoy Preethi J, H Sindhu, S Teerthanath, Bhuvaneshwari
Email(s):
rashmi.rao@manipal.edu
DOI:
10.5958/0974-360X.2020.00845.8
Address:
Nayak Apoorva1, Rao Rashmi R1, Shenoy Preethi J1, H Sindhu2, S Teerthanath3, Bhuvaneshwari4
1Department of Pharmacology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India-575001.
2Department of Biochemistry, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India-575001.
3Department of Pathology, K S Hegde Medical Academy, Nitte University, Deralakatte, Mangalore.
4Department of Pharmacology, Karpagam Faculty of Medical Sciences and Research, Coimbatore, Tamil Nadu.
*Corresponding Author
Published In:
Volume - 13,
Issue - 10,
Year - 2020
ABSTRACT:
Salvia hispanica L. (chia) seeds are a rich source of omega-3 fatty acid and polyphenolic compounds with documented antioxidant property protecting against oxidative stress, which is implicated in the pathophysiology of drug-induced hepatotoxicity. Hence, the present study evaluates the effects of Salvia hispanica seeds in an animal model of antitubercular drug induced hepatic damage. Adult wistar rats were divided into six groups; normal control, hepatotoxic control, test groups treated orally with ground Salvia hispanica seeds at doses 250,500 and 1000mg/day respectively, standard control receiving silymarin at a dose of 50mg/kg orally. Hepatotoxicity was induced by administering isoniazid with rifampicin at a dose of 100mg/kg each intraperitoneal. After administration of drugs for a period of 21 days, the blood samples were evaluated for estimation of liver enzymes (AST, ALT, and ALP), total protein, albumin and total bilirubin and the liver was dissected and sent for histopathological evaluation. The hepatotoxic group showed a significant increase in liver enzymes and total bilirubin compared to normal control. There was a significant decrease in liver enzymes and total bilirubin in the drug treated and silymarin treated groups when compared to the control. However, there was no significant difference in protein and albumin between groups. Histopathological evaluation of the liver further confirmed the hepatoprotective potential of the seeds of Salvia hispanica. Salvia hispanica seeds protect against drug induced liver injury in a murine model and the underlying mechanism can be accorded to its antioxidant activity.
Cite this article:
Nayak Apoorva, Rao Rashmi R, Shenoy Preethi J, H Sindhu, S Teerthanath, Bhuvaneshwari. Salvia hispanica (Chia) seeds afford hepatoprotection against Isoniazid and Rifampicin induced toxicity in a murine model. Research J. Pharm. and Tech. 2020; 13(10):4805-4810. doi: 10.5958/0974-360X.2020.00845.8
Cite(Electronic):
Nayak Apoorva, Rao Rashmi R, Shenoy Preethi J, H Sindhu, S Teerthanath, Bhuvaneshwari. Salvia hispanica (Chia) seeds afford hepatoprotection against Isoniazid and Rifampicin induced toxicity in a murine model. Research J. Pharm. and Tech. 2020; 13(10):4805-4810. doi: 10.5958/0974-360X.2020.00845.8 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-10-48
REFERENCES:
1. David S, Hamilton JP. Drug-induced liver injury. US Gastroenterology and Hepatology review. 2010; 6: 73–80.
2. Jeong I, Park JS, Cho YJ, Yoon HI, Song J, Lee CT, et al. Drug-induced hepatotoxicity of anti-tuberculosis drugs and their serum levels. Journal of Korean Medical Sciences. 2015;30:167-72.
3. Abera W, Cheneke W, Abebe G. Incidence of antituberculosis drug-induced hepatotoxicity and associated risk factors among tuberculosis patients in Dawro Zone, South Ethiopia: A cohort study.International Journal of Mycobacteriology. 2016;5:14-20.
4. Timbrell JA, Mitchell JR, Snodgrass WR, Nelson SD. Isoniazid hepatoxicity: the relationship between covalent binding and metabolism in vivo. Journal of Pharmacology and Experimental therapeutics. 1980;213:364-9.
5. Pandit A, Sachdeva T, Bafna P. Drug-induced hepatotoxicity: A review. Journal of Applied Pharmaceutical Sciences. 2012;02:233-43.
6. Wang C, Fan RQ, Zhang YX, Nie H, Li K. Naringenin protects against isoniazid- and rifampicin-induced apoptosis in hepatic injury. World Journal of Gastroenterology. 2016; 22:9775-83.
7. Ali NM, Yeap S, Ho W, Beh B, Tan S, Tan S. The promising future of Chia, Salvia hispanica L. Journal of biomedicine and Biotechnology. 2012:1-9.
8. Giordano E, Visioli FN. Long-chain omega 3 fatty acids: Molecular bases of potential antioxidant actions. Prostaglandins Leukotrienes and Essential Fatty Acids. 2014;90:1–4.
9. Al-Attar AM, Al-Rethea HA. Chemoprotective effect of omega-3 fatty acids on thioacetamide induced hepatic fibrosis in male rats. Saudi Journal of Biological Sciences. 2017;24:956–65.
10. Martinez-Cruz O, Paredes-Lopez O. Phytochemical profile and nutraceutical potential of chia seeds (Salvia hispanica L.) by ultra high performance liquid chromatography. Journal of Chromatography A. 2014;1346:43–8.
11. Yang SY, Hong CO, Lee GP, Kim CT, Lee KW. The hepatoprotection of caffeic acid and rosmarinic acid, major compounds of Perilla frutescens, against t-BHP-induced oxidative liver damage. Food and Chemical Toxicology. 2013;55:92-9.
12. Singh D, Cho WC, Upadhyay G. Drug-induced liver toxicity and prevention by herbal antioxidants: An Overview. Frontiers in Physiology. 2015;6:363.
13. Ullah R, Nadeem M, Khalique A, Imran M, Mehmood S, Javid A, Hussain J. Nutritional and therapeutic perspectives of Chia(Salvia hispanica L.): a review. Journal of Food Science and Technology. 2016 53:1750–8.
14. Jin F, Nieman DC, Shaetal W. Supplementation of milled chia seeds increases plasma ALA and EPA in postmenopausal women. Plant Foods For Human Nutrition.2010;67:105–10.
15. Ghosh MN. Fundamentals of experimental pharmacology. 2nd edition. Calcutta: Scientific book agency; 1984.p.154-5.
16. SA Parameswari, CM Chetty ,KB Chandrasekhar . Hepatoprotective activity of Ficus religiosa leaves against isoniazid + rifampicin and paracetamol induced hepatotoxicity. Pharmacognosy Research.2013;5:271-6.
17. Vuyyuri B, Bhagyalakshmi A, Rajyalakshmi R, Jagadeeswari S. Hepatoprotective Activity of Canthium dicoccum In Isoniazid and Rifampicin Induced Hepatotoxicity. International journal of pharmaceutical and clinical Research. 2015; 7:239-45.
18. Parasuraman S, Raveendran R, Kesavan R. Blood sample collection in small laboratory animals. J Pharmacology and Pharmacotherapy. 2010;1: 87-93.
19. Enriquez-Cortina C, Almonte-Becerril M, Clavijo-Cornejo D, Palestino-Domínguez M, Bello-Monroy O, Nuno N, Lopez A,et al. Hepatocyte growth factor protects against isoniazid/ rifampicin-induced oxidative liver damage. Toxicology. 2013;135:26-36.
20. Yew WW, Chang KC, Chan DP. Oxidative Stress and First-Line Antituberculosis Drug-Induced Hepatotoxicity. Antimicrobial Agents and Chemotherapy.2018;62: e02637-17.
21. Lian Y, Zhao J, Xu P, Wang Y, Zhao J, Jia L. Protective effects of metallothionein on isoniazid and rifampicin-induced hepatotoxicity in mice. PLoS One. 2013;8:e72058.
22. Hussain T, Gupta RK, Sweety K, Khan MS, Hussain MS, Arif M, et al. Evaluation of antihepatotoxic potential of Solanum xanthocarpum fruit extract against antitubercular drugs induced hepatopathy in experimental rodents. Asian Pacific Journal of Tropical Biomedicine. 2012;2: 454–60.
23. Okokon JE, Simeon JO, Umoh EE. Hepatoprotective activity of the extract of Homalium letestui stem against paracetamol-induced liver injury Avicenna Journal of Phytomedicine. 2017;7:27–36.
24. DS Sushma, Shenoy PJ, MS Rukmini, Salian N, Rai S, Sayeli V, Takodara S. Effect of Triphala on a murine model of Isoniazid and Rifampicin induced model of hepatotoxicity. Research Journal of Pharmaceutical Biological and Clinical Sciences. 2016;7: 618-24.
25. Rodrigues CF, Salgueiro W, Bianchini M, Veit JC, Puntel RL, Emanuelli T, et al. Salvia hispanica L. (chia) seeds oil extracts reduce lipid accumulation and produce stress resistance in Caenorhabditis elegans Nutrition and Metabolism. 2018;15:83.
26. Oliveira-Alves SC, Vendramini-Costa DB, Cazarin CB, Junior MRM, Ferreira JPB, Silva AB, Prado MA, Bronze MR. Food Chemistry. 2017;232: 295–305.
27. Scapin, G, Schmidt MM, Prestes RC, Rosa CS. Phenolics compounds, flavonoids and antioxidant activity of chia seed extracts (Salvia hispanica) obtained by different extraction conditions. International Food Research Journal 2016;23:2341-6.
28. Marineli RDS, Moraes EA, Lenquiste SA, Godoy AT, Eberlin MN, Marostica MR. Chemical characterization and antioxidant potential of Chilean chia seeds and oil (Salvia hispanica L).LWT - Food Science and Technology. 2014;59: 1304-10.
29. Marineli RDS, Lenquiste SA, Moraes EA, Marostica MR. Antioxidant potential of dietary chia seed and oil (Salvia hispanica L.) in diet-induced obese rats. Food Research International. 2015;76:666-74.