Background: Sickle cell/ß thalassemia (HbS/ ß thal) is a compound heterozygous state for ßS and either ß+ thalassemia or ß0 thalassemia. In sickle cell/ ß0 thalassemia, there is no hemoglobin A, whereas, in sickle cell/ ß+thalassemia, a variable amount of hemoglobin A is present. Aim of study was to shed a light on the expected association between sickle cell-thalassemia and oxidative stress. Methods: A total of one hundred and fifty (150) subjects were recruited for this study which consists of hundred (100) sickle cell-thalassemia subjects who 53were males and 47 were females and fifty (50) apparently healthy subjects which served as control who matched by sex and age. The present study is designed to determine (MDA) (malondialdehyde) concentration as a marker for lipid peroxidation and measurement some parameters of antioxidants including: transferrin (Tf), ceruloplasmin (Cp), and albumin (Alb), in patients with Sickle cell/ß thalassemia. Result: The means MDA and Alb were increase significant while means TF and CP decrease significant in HbS/ ß thal patients as compared to control Also it showed same result in both male and female patients with HbS/ ß thal as compared with their healthy counterparts. There were no significant difference between male and female patients in means MDA, CP and Alb except mean TF. The result revealed that no significant difference in means MDA while significant difference in TF, CP and Alb in patient among age group. The means MDA, CP and Alb were not significant difference between male and female patients in each age group while there was found significant difference in mean TF between male and female patient in 1-10 age group only. Conclusion: The increase in the oxidative stress level, especially MDA in sickle cell thalassemia patients compared to healthy individuals, confirms that oxidative stress is involved in the pathogenesis of Sickle cell/ß thalassemia disease.
Cite this article:
Rabab Ali Shnawa Al-Mosawi, Khalid G. Al-Fartosi. Oxidant and Antioxidant Status of patients with Sickle cell –β Thalassemia in Thi-Qar Province, Iraq. Research J. Pharm. and Tech. 2020; 13(10):4796-4800. doi: 10.5958/0974-360X.2020.00843.4
Rabab Ali Shnawa Al-Mosawi, Khalid G. Al-Fartosi. Oxidant and Antioxidant Status of patients with Sickle cell –β Thalassemia in Thi-Qar Province, Iraq. Research J. Pharm. and Tech. 2020; 13(10):4796-4800. doi: 10.5958/0974-360X.2020.00843.4 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-10-46
1. Mukherjee MB, Nadkarni AH, Gorakshakar AC, Ghosh K, Mohanty D, Colah RB. Clinical, hematologic and molecular variability of sickle cell-β thalassemia in western India. Indian J Hum Genet [Internet]. 2010 Sep;16(3):154–8. Available from: https://www.ncbi.nlm.nih.gov/pubmed/ 21206704.
2. Dhawan D, Chaudhary S. Prenatal Screening for Co-Inheritance of Sickle Cell Anemia and β-Thalassemia Traits. Clin Med Biochem Open Access. 2016;02 (01):2–4.
3. Figueiredo MS. The compound state: Hb S/beta-thalassemia. Rev Bras Hematol Hemoter. 2015;37(3):150–2.
4. Queiroz RF, Lima ES. Oxidative stress in sickle cell disease. Rev Bras Hematol Hemoter [Internet]. 2013;35(1):16–7.Availablefrom: https://www.ncbi.nlm.nih. gov/pub med/ 23580878.
5. Belcher JD, Beckman JD, Balla G, Balla J, Vercellotti G. Heme degradation and vascular injury. Antioxid Redox Signal. 2010;12(2):233–48.
6. Fong K-L, McCay PB, Poyer JL, Keele BB, Misra H. Evidence that peroxidation of lysosomal membranes is initiated by hydroxyl free radicals produced during flavin enzyme activity. J Biol Chem. 1973;248(22):7792–7.
7. Starr RT. Use of an alumina column in estimating total iron-binding capacity. Clin Chem. 1980;26(1):156–8.
8. Menden C; Boian J; Murthy L; and Petering H. G . Plasma antioxidant. Anal Lett.1977,10, 197.
9. Webster D. Role of oxidative stress in diabetic complication. Clin. Chem. J.1974, 53 ,109.
10. Morris CR, Suh JH, Hagar W, Larkin S, Bland DA, Steinberg MH, et al. Erythrocyte glutamine depletion, altered redox environment, and pulmonary hypertension in sickle cell disease. Blood. 2008;111(1):402–10.
11. Titus J, Chari S, Gupta M, Parekh N. Pro-oxidant and anti-oxidant status in patients of sickle cell anaemia. Indian J Clin Biochem. 2004;19(2):168.
12. Okorie C, Nwagha T, Ejezie F. Assessment of some indicators of oxidative stress in nigerian sickle cell anemic patients. Ann Afr Med [Internet]. 2018 Jan 1;17(1):11–6. Available from: http://www.annalsafrmed.org/article.asp?issn= 1596-3519
13. Oztas Y, Yalcinkaya A. Oxidative alterations in sickle cell disease: Possible involvement in disease pathogenesis. World J Hematol. 2017;6(3):55–61.
14. Rogers JT, Bridges KR, Durmowicz GP, Glass J, Auron PE, Munro HN. Translational control during the acute phase response. Ferritin synthesis in response to interleukin-1. J Biol Chem. 1990;265(24):14572–8.
15. Tórsdóttir G, Kristinsson J, Gudmundsson G, Snaedal J, Jóhannesson T. Copper, ceruloplasmin and superoxide dismutase (SOD) in amyotrophic lateral sclerosis. Pharmacol Toxicol. 2000;87(3):126–30.
16. Biemond P, Swaak AJG, Koster JF. Protective factors against oxygen free radicals and hydrogen peroxide in rheumatoid arthritis synovial fluid. Arthritis Rheum Off J Am Coll Rheumatol. 1984;27(7):760–5.
17. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev [Internet]. 2010;4(8):118–26. Available from: https://www.ncbi.nlm.nih. gov/pubmed/ 22228951.
18. M. Goralska, J. Ferrell, J. Harned, M. Lall, S. Nagar, L.N. Fleisher MCM. Iron metabolism in the eye, a review M. 2013;88(2):204–15.
19. Famodu AA, Omodiale P, Adedeji MO, Reid HL. Serum protein study in adult Nigerians with sickle-cell anaemia (HbSS). Med Sci Res. 1987;15(15):913–4.
20. Oyeyemi AO, Sonuga AA. Assessment of plasma albumin, total protein and prostaglandins in sickle cell children in Ekiti state, nigeria. 2018;9(2):1166–75.
21. Tripathi S, Dadsena R, Kumar A. Study of certain Biochemical parameters in patients of sickle cell anemia. Adv Biores. 2011;2(2):79–81.
22. Jemikalajah J, Matthew A, Digban k. Evaluation of some biochemical parameters of sickle cell disease (scd) patients in benin city. Vol. 2, Bayero Journal of Medical Laboratory Science. 2017. 237–243 p.
23. Roche M, Rondeau P, Singh NR, Tarnus E, Bourdon E. The antioxidant properties of serum albumin. FEBS Lett. 2008;582(13):1783–7.
24. Halliwell B. Albumin—an important extracellular antioxidant? Biochem Pharmacol. 1988;37(4):569–71.
25. Halliwell B. Superoxide‐dependent formation of hydroxyl radicals in the presence of iron chelates: Is it a mechanism for hydroxyl radical production in biochemical systems? FEBS Lett. 1978;92(2):321–6.
26. Vincent O, Oluwaseyi B, James B, Saidat L. Coinheritance of B-Thalassemia and Sickle Cell Anaemia in Southwestern Nigeria. Ethiop J Health Sci [Internet]. 2016;26(6):517–22.Available from: http://www.ncbi. nlm.nih.gov /pubmed/28450766%0Ahttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5389070.