Author(s): Shabna Roupal Morais, R. Ram Narayanan, K. P. Sushmitha, N. Suchithra, Shruti Shankar, M. Sugumar

Email(s): ramraanapharma@gmail.com

DOI: 10.5958/0974-360X.2020.00840.9   

Address: Shabna Roupal Morais, R. Ram Narayanan*, K. P. Sushmitha, N. Suchithra, Shruti Shankar, M. Sugumar
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), Porur, Chennai - 600116.
*Corresponding Author

Published In:   Volume - 13,      Issue - 10,     Year - 2020


ABSTRACT:
Over the years, the pursuit for new antioxidant compounds from natural products has mounted, due to health concerns regarding the potential toxic and side effects spawn from synthetic antioxidants. In this study the antioxidant potential and the cytotoxic activity was studied using hydroxyl radical scavenging assay and MTT assay using MCF 7 breast cancer cell lines. The hydroxyl radical scavenging potential of various solvent extracts of A.spicifera showed that hydroxyl radical scavenging capacity was increased with decreasing concentration of sample extracts. In the present investigation, out of the four extracts (hexane, ethyl acetate, chloroform, methanol) of A. spicifera methanol showed potential hydroxyl radical scavenging activity than vitamin E in the range of 94.84-99.81% at the concentration range of 50µg/ml-1000µg/ml where the percentage inhibition increased with decrease in concentration. While scavenging hydroxyl radical, the ability of methanol extract (99.81%) to scavenge the hydroxyl radical was the highest among other extracts and Vitamin E. The methanolic extract of A.spicifera was evaluated for in-vitro anti-proliferative assay against MCF-7 cell line, where the cells were exposed to different concentrations of the extract. It is inferred from the results that the appearance of cytotoxic changes to the extract was in a concentration dependant manner. The results also indicate that the IC50 value of the extract was found to be 47.58µg/ml.


Cite this article:
Shabna Roupal Morais, R. Ram Narayanan, K. P. Sushmitha, N. Suchithra, Shruti Shankar, M. Sugumar. In vitro Biological Evaluation of Acanthophora spicifera. Research J. Pharm. and Tech. 2020; 13(10):4777-4783. doi: 10.5958/0974-360X.2020.00840.9

Cite(Electronic):
Shabna Roupal Morais, R. Ram Narayanan, K. P. Sushmitha, N. Suchithra, Shruti Shankar, M. Sugumar. In vitro Biological Evaluation of Acanthophora spicifera. Research J. Pharm. and Tech. 2020; 13(10):4777-4783. doi: 10.5958/0974-360X.2020.00840.9   Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-10-43


REFERENCES:
1.    "The Significance of Seaweed | KAUST Discovery". Discovery.kaust.edu.sa. N.P., 2016. Web. 19 Dec. 2016.
2.    "Main Characteristics of Rhodophyceae (Red Algae)". Plantscience4u.com. N.P., 2016. Web. 21 Dec. 2016.
3.    Nurul ZA, Darah I, Shaida SF, Nor SA. Screening for antimicrobial activity of various extracts of Acanthophora spicifera (Rhodomelaceae, Ceramiales) from Malaysian waters. Res J Biol Sci. 2010;5:368-75.
4.    Hetta M, Hassan S, Abdel-Tawab S, Bastawy M, Mahmoud B. Hypolipidaemic effect of Acanthophora spicifera (red alga) and Cystoseiratrinode (Brown alga) on albino rats. Iranian Journal of Science and Technology (Sciences). 2009 Dec 12;33(4):287-97.
5.    Pandian P, Selvamuthukumar S, Manavalan R, Parthasarthy V. Screening of antibacterial and antifungal activities of red marine algae Acanthaphora spicifera (Rhodophyceae). J. Biomed. Sci. Res.2011;3(3):444-8.
6.    Nogueira CC, de Palmer Paixão IC, Cirne-Santos CC, Stephens PR, Villaça RC, de Souza Pereira H, Teixeira VE. Anti-HIV-1 activity in human primary cells and Anti-HIV-1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera Journal of Medicinal Plants Research. 2016 Sep 17;10(35):621-5.
7.    Van Meerloo J, et al. "Cell Sensitivity Assays: The MTT Assay. - Pubmed - NCBI". Ncbi.nlm.nih.gov. N.p., 2016. Web. 19 Dec. 2016.
8.    Abirami RG, Kowsalya S. Phytochemical screening, microbial load and antimicrobial activity of underexploited seaweeds. International Research Journal of Microbiology. 2012;3(10):328-32.
9.    Lavakumar V, Ahamed KF, Ravichandiran V. Anticancer and antioxidant effect of Acanthophora spicifera against EAC induced carcinoma in mice. J Pharm Res. 2012 Mar;5:1503-7.
10.    Zakaria NA, Ibrahim D, Sulaiman SF, Supardy A. Assessment of antioxidant activity, total phenolic content and in vitro toxicity of Malaysian red seaweed, Acanthophora spicifera. J Chem Pharm Res. 2011;3(3):182-91.
11.    Patel DK, Kumar R, Laloo D, Sairam K, Hemalatha S. Evaluation of phytochemical and antioxidant activities of the different fractions of Hybanthus enneaspermus (Linn.) F. Muell. (Violaceae). Asian Pac J Trop Med 2011;4:391-396.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank

Journal Policies & Information


Recent Articles




Tags


Not Available