Shabna Roupal Morais, R. Ram Narayanan, K. P. Sushmitha, N. Suchithra, Shruti Shankar, M. Sugumar
Shabna Roupal Morais, R. Ram Narayanan*, K. P. Sushmitha, N. Suchithra, Shruti Shankar, M. Sugumar
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), Porur, Chennai - 600116.
Volume - 13,
Issue - 10,
Year - 2020
Over the years, the pursuit for new antioxidant compounds from natural products has mounted, due to health concerns regarding the potential toxic and side effects spawn from synthetic antioxidants. In this study the antioxidant potential and the cytotoxic activity was studied using hydroxyl radical scavenging assay and MTT assay using MCF 7 breast cancer cell lines. The hydroxyl radical scavenging potential of various solvent extracts of A.spicifera showed that hydroxyl radical scavenging capacity was increased with decreasing concentration of sample extracts. In the present investigation, out of the four extracts (hexane, ethyl acetate, chloroform, methanol) of A. spicifera methanol showed potential hydroxyl radical scavenging activity than vitamin E in the range of 94.84-99.81% at the concentration range of 50µg/ml-1000µg/ml where the percentage inhibition increased with decrease in concentration. While scavenging hydroxyl radical, the ability of methanol extract (99.81%) to scavenge the hydroxyl radical was the highest among other extracts and Vitamin E. The methanolic extract of A.spicifera was evaluated for in-vitro anti-proliferative assay against MCF-7 cell line, where the cells were exposed to different concentrations of the extract. It is inferred from the results that the appearance of cytotoxic changes to the extract was in a concentration dependant manner. The results also indicate that the IC50 value of the extract was found to be 47.58µg/ml.
Cite this article:
Shabna Roupal Morais, R. Ram Narayanan, K. P. Sushmitha, N. Suchithra, Shruti Shankar, M. Sugumar. In vitro Biological Evaluation of Acanthophora spicifera. Research J. Pharm. and Tech. 2020; 13(10):4777-4783. doi: 10.5958/0974-360X.2020.00840.9
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