Author(s): Dhrubajyoti Sarkar, Subhashis Debnath, Sekhar Kumar Bose


DOI: 10.5958/0974-360X.2020.00800.8   

Address: Dhrubajyoti Sarkar1*, Subhashis Debnath2, Sekhar Kumar Bose1
1Department of Pharmaceutical Technology, NSHM Knowledge Campus, 124, B.L Saha Road, Kolkata-700053, India.
2Royal School of Pharmacy, The Assam Royal Global University, Guwahati- 781035.
*Corresponding Author

Published In:   Volume - 13,      Issue - 10,     Year - 2020

The objective of this study was to conduct phytochemical characterization and antioxidant evaluation of black tea extract (BTE) obtained from Camelia sinensis. Fermented extract from leaves were obtained for phytochemical screening and evaluation of In-vitro antioxidant potential. Phytochemical data revealed the presence of several metabolites, notably from the flavonoid and phenolic class. In the antioxidant activity using the DPPH method, nitric oxide method, superoxide method, hydroxyl radical method and reductive method the enriched extract presented significant IC50 values when compared with ascorbic acid as a standard. These results contribute to the continuity of studies on the chemical and biological composition of the species.

Cite this article:
Dhrubajyoti Sarkar, Subhashis Debnath, Sekhar Kumar Bose. Phytochemical analysis and assessment of Antioxidant properties of black tea extract obtained from Camellia sinensis. Research J. Pharm. and Tech. 2020; 13(10):4539-4544. doi: 10.5958/0974-360X.2020.00800.8

Dhrubajyoti Sarkar, Subhashis Debnath, Sekhar Kumar Bose. Phytochemical analysis and assessment of Antioxidant properties of black tea extract obtained from Camellia sinensis. Research J. Pharm. and Tech. 2020; 13(10):4539-4544. doi: 10.5958/0974-360X.2020.00800.8   Available on:

1.    Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8):118-126.
2.    Arulselvan P, Fard MT, Tan WS, et al. Role of Antioxidants and Natural Products in Inflammation. Oxid Med Cell Longev. 2016;2016.
3.    Xu D-P, Li Y, Meng X, et al. Natural Antioxidants in Foods and Medicinal Plants: Extraction, Assessment and Resources. Int J Mol Sci. 2017;18(1).
4.    Gülçin İ. Antioxidant activity of food constituents: an overview. Arch Toxicol. 2012;86(3):345-391.
5.    Lichota A, Gwozdzinski K. Anticancer Activity of Natural Compounds from Plant and Marine Environment. Int J Mol Sci. 2018;19(11).
6.    Das N, Islam ME, Jahan N, et al. Antioxidant activities of ethanol extracts and fractions of Crescentia cujete leaves and stem bark and the involvement of phenolic compounds. BMC Complement Altern Med. 2014; 14:45.
7.    Xia E-H, Li F-D, Tong W, et al. Tea Plant Information Archive: a comprehensive genomics and bioinformatics platform for tea plant. Plant Biotechnol J. 2019;17(10):1938-1953.
8.    Hao X, Horvath DP, Chao WS, Yang Y, Wang X, Xiao B. Identification and Evaluation of Reliable Reference Genes for Quantitative Real-Time PCR Analysis in Tea Plant (Camellia sinensis (L.) O. Kuntze). International Journal of Molecular Sciences. 2014;15(12):22155-22172.
9.    Hilal Y, Engelhardt U. Characterisation of white tea – Comparison to green and black tea. J Verbr Lebensm. 2007;2(4):414-421.
10.    Sarkar D, Dutta D, Mandal SC, Bose S. Role of Tea Polyphenols in Diabetes. 2018:9.
11.    Zhang J, Cui H, Jiang H, et al. Rapid determination of theaflavins by HPLC with a new monolithic column. Czech Journal of Food Sciences. 2019;37 (2019) (No. 2):112-119.
12.    Annapandian VM, Rajagopal SS. Phytochemical Evaluation and In vitro Antioxidant Activity of Various Solvent Extracts of Leucas aspera (Willd.) Link Leaves. Free Radicals and Antioxidants. 2017;7(2):166-171.
13.    Ghoulami S, Il Idrissi A, Fkih-Tetouani S. Phytochemical study of Mentha longifolia of Morocco. Fitoterapia. 2001;72(5):596-598.
14.    Mathew S, Abraham TE. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food and Chemical Toxicology. 2006;44(2):198-206.
15.    Valentão P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. Antioxidant Activity of Centaurium erythraea Infusion Evidenced by Its Superoxide Radical Scavenging and Xanthine Oxidase Inhibitory Activity. J Agric Food Chem. 2001;49(7):3476-3479.
16.    Sueoka N, Suganuma M, Sueoka E, et al. A New Function of Green Tea: Prevention of Lifestyle-related Diseases. Annals of the New York Academy of Sciences. 2001;928(1):274-280.
17.    Oršolić N, Goluža E, Đikić D, et al. Role of flavonoids on oxidative stress and mineral contents in the retinoic acid-induced bone loss model of rat. Eur J Nutr. 2014;53(5):1217-1227.
18.    Sies H. Oxidative stress: a concept in redox biology and medicine. Redox Biology. 2015;4: 180-183.
19.    Bertin R, Chen Z, Marin R, et al. Activity of myricetin and other plant-derived polyhydroxyl compounds in human LDL and human vascular endothelial cells against oxidative stress. Biomedicine and Pharmacotherapy. 2016;82: 472-478.
20.    Förstermann Ulrich, Xia Ning, Li Huige. Roles of Vascular Oxidative Stress and Nitric Oxide in the Pathogenesis of Atherosclerosis. Circulation Research. 2017;120(4):713-735.
21.    Indo HP, Yen H-C, Nakanishi I, et al. A mitochondrial superoxide theory for oxidative stress diseases and aging. Journal of Clinical Biochemistry and Nutrition. 2015;56(1):1-7.

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