Author(s): Lilima Nath, Laldinchhana, Abhijit Deb Choudhury, Himal Barakoti, Chanam Melody Devi

Email(s): melodykhr90@gmail.com

DOI: 10.5958/0974-360X.2020.00009.8   

Address: Lilima Nath1, Laldinchhana1, Abhijit Deb Choudhury1, Himal Barakoti2, Chanam Melody Devi*1,2
1Department of Pharmacy, Regional Institute of Paramedical and Nursing Sciences, Zemabawk, Aizawl, Mizoram, India - 796017
2Faculty of Pharmaceutical Science, Assam Down Town University, Guwahati, Assam, India – 781026
*Corresponding Author

Published In:   Volume - 13,      Issue - 1,     Year - 2020


ABSTRACT:
Amphotericin B (AmB) is a polyene macrolide antibiotic which shows antifungal activity against the widest spectrum of fungal infections and in certain protozoan infections with no major problems of resistance and remains effective till date. Literature has given insight on the preparation of different novel formulations of AmB as lipid formulations and polymeric nanoparticles using UV-VIS spectrophotometry and HPLC method, but details of peak characteristics and validation data are not described. This research was focused to develop a suitable validation method of AmB and acquire consistent, reliable and accurate data on linearity, detection limit, quantification limit, accuracy and precision using a UV-VIS spectrophotometer. The UV-VIS spectrum of AmB was recorded with the study of linearity, accuracy, precision, detection and quantification limit was carried for the validation of the process. The method showed good linearity with regression of y =0.1355x (R2 = 0.9992) at 408 nm. The repeatability (inter-day) and intermediate precision (intra-day) precision studies revealed that the method is precise and reliable where all the RSD values were ?2%. Method accuracy showed % accuracy value for all the three concentration levels ranged from 103.5% to 106.5% with standard deviation ranging from ± 0.763 to ± 1.12 that a small change in the concentration of the drug could be accurately determined with high accuracy. Limit of detection and limit of quantification were 0.391 (µg/ml) and 1.187 (µg/ml) respectively. The proposed method is simple and could be regarded as a reasonably feasible technique in the routine estimation of AmB.


Cite this article:
Lilima Nath, Laldinchhana, Abhijit Deb Choudhury, Himal Barakoti, Chanam Melody Devi. Development and Validation of UV-Vis Spectrophotometric Method for Estimation of Amphotericin B. Research J. Pharm. and Tech. 2020; 13(1):55-59. doi: 10.5958/0974-360X.2020.00009.8

Cite(Electronic):
Lilima Nath, Laldinchhana, Abhijit Deb Choudhury, Himal Barakoti, Chanam Melody Devi. Development and Validation of UV-Vis Spectrophotometric Method for Estimation of Amphotericin B. Research J. Pharm. and Tech. 2020; 13(1):55-59. doi: 10.5958/0974-360X.2020.00009.8   Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-1-9


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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