ABSTRACT:
The purpose of this research was to test the anti-adipogenic activity and to identify the active compound of the leaf of Guazuma ulmifolia. The activity of anti-adipogenic of the compound of G. ulmifolia leaf was tested using primary cultures of Wistar strain white mouse preadipocytes. Isolation of the active compound was carried out in the following way. The powder of G. ulmifolia leaf was defated with n-hexane followed by extraction with 70% ethanol. The extract was concentrated and fractionated by liquid-liquid partition using chloroform and ethyl acetate solvents. The ethyl acetate fraction was loaded into a Vacuum Liquid Chromatography (VLC) followed by a reverse phase preparative layer chromatography to obtain a pure isolate. Isolated compound inhibited preadipocytes proliferation and differentiation. Intensive spectroscopic and spectrometric analysis suggested molecular structure of a tiliroside which agreed with previously reported data. The study successfully revealed the traditional claims of the anti-obesity agent of a leaf of G. ulmifolia through inhibition of preadipocytes proliferation and differentiation.