Author(s): Nuri, Bambang Prajogo, Ari S. Nugraha, Sukardiman

Email(s): nuri.farmasi@unej.ac.id , maman_ht@yahoo.com

DOI: 10.5958/0974-360X.2020.00080.3   

Address: Nuri1,2*, Bambang Prajogo4, Ari S. Nugraha3, Sukardiman4*
1Doctoral Student of Pharmaceutical Sciences, Faculty of Pharmacy, Airlangga University, Indonesia 60286
2Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Jember, Indonesia 68121
3Drug Utilisation and Discovery Research Group, Faculty of Pharmacy, University of Jember, Indonesia 68121
4Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University, Indonesia 60286
*Corresponding Author

Published In:   Volume - 13,      Issue - 1,     Year - 2020


ABSTRACT:
The purpose of this research was to test the anti-adipogenic activity and to identify the active compound of the leaf of Guazuma ulmifolia. The activity of anti-adipogenic of the compound of G. ulmifolia leaf was tested using primary cultures of Wistar strain white mouse preadipocytes. Isolation of the active compound was carried out in the following way. The powder of G. ulmifolia leaf was defated with n-hexane followed by extraction with 70% ethanol. The extract was concentrated and fractionated by liquid-liquid partition using chloroform and ethyl acetate solvents. The ethyl acetate fraction was loaded into a Vacuum Liquid Chromatography (VLC) followed by a reverse phase preparative layer chromatography to obtain a pure isolate. Isolated compound inhibited preadipocytes proliferation and differentiation. Intensive spectroscopic and spectrometric analysis suggested molecular structure of a tiliroside which agreed with previously reported data. The study successfully revealed the traditional claims of the anti-obesity agent of a leaf of G. ulmifolia through inhibition of preadipocytes proliferation and differentiation.


Cite this article:
Nuri, Bambang Prajogo, Ari S. Nugraha, Sukardiman. Anti-Adipogenic compound from Guazuma ulmifolia Leaf. Research J. Pharm. and Tech. 2020; 13(1):411-415. doi: 10.5958/0974-360X.2020.00080.3

Cite(Electronic):
Nuri, Bambang Prajogo, Ari S. Nugraha, Sukardiman. Anti-Adipogenic compound from Guazuma ulmifolia Leaf. Research J. Pharm. and Tech. 2020; 13(1):411-415. doi: 10.5958/0974-360X.2020.00080.3   Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-1-80


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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