Author(s):
Hasyrul Hamzah, Triana Hertiani, Sylvia Utami Tunjung Pratiwi, Yosi Bayu Murti, Titik Nuryastuti
Email(s):
hertiani@ugm.ac.id
DOI:
10.5958/0974-360X.2020.00075.X
Address:
Hasyrul Hamzah1, Triana Hertiani2*, Sylvia Utami Tunjung Pratiwi2, Yosi Bayu Murti2,Titik Nuryastuti3
1Program Doctoral Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55281 Indonesia
2Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta,
55281 Indonesia.
3Department of Microbiology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia,
55281 Indonesia.
*Corresponding Author
Published In:
Volume - 13,
Issue - 1,
Year - 2020
ABSTRACT:
Demethoxycurcumin is a pure compound from (Curcuma longa Linn.) that has antimicrobial activity, yet the antibiofilm activity against Candida albicans (C. albicans) has never been tested yet. The invention of new antibiofilm candidates against C.albicans biofilm is a major challenge that needs to be managed in order to prevent biofilm-related infections. This study aimed to understand the effectiveness of Demethoxycurcumin in its ability to inhibit and degrade ATCC 10231 from C. albicans. The biofilm inhibition and degradation testing were determined with microtiter broth method, while the effectiveness of Demethoxycurcumin antibiofilm activity towards biofilm was analyzed by calculating the minimum biofilm concentration and the minimum value of biofilm eradication concentration. The morphology of Demethoxycurcumin on C. albicans biofilm was assessed by scanning electron microscope. Demethoxycurcumin1% gave inhibition activity of 80 % on C. albicans biofilm forming in the mid-phase for 82.70 % ± 0.02 and 50 % in the maturing phase for 62.94 % ± 0.01 and this was better than control medication nystatin in the mid-phase and maturing phase for 70.67 % ± 0.01 and 55.64 % ± 1.24. The results also demonstrated that Demethoxycurcumin activity could degrade 50 % C. albicans biofilm for 57.25 % ± 0.01 and damage the extracellular polymeric substances’ matrix of the C. albicans biofilm. Accordingly, Demethoxycurcumin has great potential to be developed as one of the new candidates for antibiofilm medications against C.albicans biofilm.
Cite this article:
Hasyrul Hamzah, Triana Hertiani, Sylvia Utami Tunjung Pratiwi, Yosi Bayu Murti,Titik Nuryastuti. The Inhibition and Degradation Activity of Demethoxycurcumin as Antibiofilm on C. albicans ATCC 10231. Research J. Pharm. and Tech. 2020; 13(1): 377-382. doi: 10.5958/0974-360X.2020.00075.X
Cite(Electronic):
Hasyrul Hamzah, Triana Hertiani, Sylvia Utami Tunjung Pratiwi, Yosi Bayu Murti,Titik Nuryastuti. The Inhibition and Degradation Activity of Demethoxycurcumin as Antibiofilm on C. albicans ATCC 10231. Research J. Pharm. and Tech. 2020; 13(1): 377-382. doi: 10.5958/0974-360X.2020.00075.X Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-1-75