Author(s): Fedik Abdul Rantam, Alexander Patera Nugraha, Ferdiansyah Ferdiansyah, Purwati Purwati, Candra Bumi, Helen Susilowati, Eryk Hendrianto, Dwikora Novembri, Utomo Heri Suroto, Christijogo Sumartono, Rosy Setiawati8, Cita Rosita Prakoeswa, Diah Mira Indramaya

Email(s): fedik-a-r@fkh.unair.ac.id

DOI: 10.5958/0974-360X.2020.00056.6   

Address: Fedik Abdul Rantam1,2*, Alexander Patera Nugraha2,3,4, Ferdiansyah Ferdiansyah5, Purwati Purwati2,6, Candra Bumi2, Helen Susilowati2, Eryk Hendrianto2, Dwikora Novembri Utomo5 Heri Suroto5, Christijogo Sumartono7, Rosy Setiawati8, Cita Rosita Prakoeswa9, Diah Mira Indramaya9
1Virology and Immunology Laboratory, Microbiology Department., Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60132, Indonesia.
2Stem Cell Research and Development Center, Universitas Airlangga, Surabaya 60115, Indonesia.
3Doctoral Student of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya 60132, Indonesia.
4Orthodontic Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, Indonesia.
5Orthopedic and Traumatology Department
RSUD Dr. Soetomo Hospital/Faculty of Medicine Universitas Airlangga, Surabaya 60132, Indonesia.
6Health Department, Faculty of Vocation, Universitas Airlangga, Surabaya, 60132 Indonesia
7Anasthesiology and Reanimation

Published In:   Volume - 13,      Issue - 1,     Year - 2020


ABSTRACT:
Human Adipose Derived Mesenchymal Stem Cells (HADMSCs) and Human Hair Follicle Derived Mesenchymal Stem Cells (HHFDMSCs) have attracted great interest because of their multilineage differentiation potential, self-renewal properties, and their possible use of cell and gene therapies. This present study to investigate the neurogenic differentiation ability of hADMSCs and hHFDMSCs induced by Epidermal Growth Factors (EGF), Fibroblast Growth Factor (FGF), Platelet Derived Growth Factor (PDGF) and Forskolin. This study was true experimental with longitudinal study design. The sample size determined with minimal sample size formula and it was randomly chosen. These studies employed an in vitro design for the expansion and proliferation of Mesenchymal Stem Cells (MSCs) and examined the heterogeneity of these cells using the markers CD105, CD90, OCT4, and SOX2. MSCs from adipose tissue and hair follicles were induced with EGF, FGF, PDGF and Forskolin to differentiate and generate neurons. The capacity of MSCs to generate neurons were verified using glial fibrillary acidic protein, nestin, and ß-tubulin III . The expression of neural markers and morphological changes in Mesenchymal stem cells from hADMSCs and hHFDMSCs were confirmed. hADMSCs and hHFDMSCs share a similar capacity to differentiate and generate neurons, which is beneficial for the development of neuronal restoration for future therapies for patients suffering from neurological diseases.


Cite this article:
Fedik Abdul Rantam, Alexander Patera Nugraha, Ferdiansyah Ferdiansyah, Purwati Purwati, Candra Bumi, Helen Susilowati, Eryk Hendrianto, Dwikora Novembri, Utomo Heri Suroto, Christijogo Sumartono, Rosy Setiawati8, Cita Rosita Prakoeswa, Diah Mira Indramaya. A Potential Differentiation of Adipose and Hair Follicle-derived Mesenchymal Stem Cells to Generate Neurons Induced with EGF, FGF, PDGF and Forskolin. Research J. Pharm. and Tech. 2020; 13(1): 275-281 doi: 10.5958/0974-360X.2020.00056.6

Cite(Electronic):
Fedik Abdul Rantam, Alexander Patera Nugraha, Ferdiansyah Ferdiansyah, Purwati Purwati, Candra Bumi, Helen Susilowati, Eryk Hendrianto, Dwikora Novembri, Utomo Heri Suroto, Christijogo Sumartono, Rosy Setiawati8, Cita Rosita Prakoeswa, Diah Mira Indramaya. A Potential Differentiation of Adipose and Hair Follicle-derived Mesenchymal Stem Cells to Generate Neurons Induced with EGF, FGF, PDGF and Forskolin. Research J. Pharm. and Tech. 2020; 13(1): 275-281 doi: 10.5958/0974-360X.2020.00056.6   Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-1-56


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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