Payel Mitra, Arun Kumar Dutta, Narayan Chandra Mandal, Krishnendu Acharya
Payel Mitra1,2, Arun Kumar Dutta1, Narayan Chandra Mandal2, Krishnendu Acharya1*
1Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, West Bengal – 700019, India.
2Mycology and Plant Pathology Laboratory, Department of Botany, Visva–Bharati, Santiniketan – 731235, India.
Volume - 12,
Issue - 9,
Year - 2019
Free radical scavenging activity and lipid peroxidation inhibition of polyphenol rich extract of Termitomyces medius, was investigated using various in–vitro assays. Sample was collected and morphological characters recorded. DNA sequencing was done and extracts prepared. Different antioxidant assays were performed for the polyphenol rich extract of Termitomyces medius. Even bioactive compounds were determined and HPLC conducted. Highest activity was observed in relation to hydroxyl radical scavenging activity where EC50 was found at a concentration of 0.0195 mg/ml. The chelating ability of ferrous ion, DPPH and superoxide radical activity in terms of EC50 values were found in the range 0.4–0.6 mg/ml. Whereas EC50 value for reducing power and inhibition of lipid peroxidation showed a higher range of 1.55–1.65 mg/ml. On the other hand estimated phytochemical component analysis showed total phenol content (mostly pyrogallol) was much higher than that of ß–carotene and lycopene contents. As a result, due to its strong antioxidative properties, T. medius is thought to be a potential source of natural antioxidants.
Cite this article:
Payel Mitra, Arun Kumar Dutta, Narayan Chandra Mandal, Krishnendu Acharya. Phytochemical Study and Antioxidative property of Polyphenol Rich Fraction from Termitomyces medius. Research J. Pharm. and Tech 2019; 12(9):4287-4294. doi: 10.5958/0974-360X.2019.00737.6