ABSTRACT:
Objective: Objective of the study was to evaluate antimicrobial activity against clinical isolates of human pathogen, physiochemical and Phytochemical analysis of Lantana indica Roxb. Methods: The aqueous, methanol, acetone and chloroform fractions of Lantana indica were investigated for antimicrobial properties using pathogenic species of bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Streptococcus pyrogens, Alcaligene fecalis, Salmonella typhi, Bacillu cereus, Bacillus subtilis, Micrococcus luteus, Micrococcus roseus, Streptococcus faecalis, Klebsiella pneumonia, Proteus vulgaris, Proteus mirabilis, Escherichia coli) and fungi (Aspegillus fumigatus, A. niger, A. flavus, Candida albicans and C. tropicals, Microsporum canis, Epidermophyton floccossum, Trichophyton rubrum). Results: All the fractions of plant exhibited variable antimicrobial activity, however, the fraction prepared by extraction with water was found to be endowed with maximum antimicrobial activity by disc diffusion assay. The result of phytochemical shows that Lantana indica contain good source of elements and vitamin. The MICs by microbroth dilution assay was found to be ranged from 0.625 to 10.0 mg/ml in most of the fractions. Acetone extract was found to be least active against the fungal pathogens. Conclusion: In present study, all the four extracts of L. indica were found to be having moderate to high activity against most of the microbial species investigated; therefore, characterization of various extracts may be useful in identification of some novel antimicrobial compound having less toxicity.