Author(s):
Fathima Qurratul Ayeen, Ruheena Yasmeen, Humera Badar
Email(s):
ayeenfathima@gmail.com
DOI:
10.5958/0974-360X.2019.00577.8 
Address:
Fathima Qurratul Ayeen1, Dr. Ruheena Yasmeen2, Humera Badar3
1Assistant Professor, Department of Pharmaceutical Analysis and Quality Assurance, Nizam Institute of Pharmacy, Deshmukhi (V), Pochampally (M), Behind Mount Opera, Yadadri Bhuvanagiri (Dist)-508284, Telangana, India.
2Assistant Professor, Department of Pharm. D (Pharmacy Practice), Nizam Institute of Pharmacy,
Deshmukhi (V), Pochampally (M), Behind Mount Opera, Yadadri Bhuvanagiri (Dist)-508284, Telangana, India.
3Assistant Professor, Department of Pharmaceutical Analysis and Quality Assurance, Nizam Institute of Pharmacy, Deshmukhi (V), Pochampally (M), Behind Mount Opera, Yadadri Bhuvanagiri (Dist)-508284, Telangana, India.
*Corresponding Author
Published In:
Volume - 12,
Issue - 7,
Year - 2019
ABSTRACT:
A novel simple, accurate, precise and selective high performance liquid chromatographic (HPLC) method was developed and validated for simultaneous estimation of Ritonavir and Lopinavir in tablet dosage form. An isocratic, reverse phase HPLC method was developed and validated using Hypersil C18 (250 mm×4.6 mm i.d.) 5µm, column and orthophosphoric acid pH3 and methanol (40:60) as mobile phase and detection is carried out at a wavelength of 273nm.The retention time for RITO and LOPI were 3.3±0.1 min and 4.7±0.1min respectively. The method was validated with respect to linearity, precision, accuracy and robustness.
Cite this article:
Fathima Qurratul Ayeen, Ruheena Yasmeen, Humera Badar. Development and Validation of RP-HPLC Method for Determination of Ritonavir and Lopinavir. Research J. Pharm. and Tech. 2019; 12(7):3413-3417. doi: 10.5958/0974-360X.2019.00577.8
Cite(Electronic):
Fathima Qurratul Ayeen, Ruheena Yasmeen, Humera Badar. Development and Validation of RP-HPLC Method for Determination of Ritonavir and Lopinavir. Research J. Pharm. and Tech. 2019; 12(7):3413-3417. doi: 10.5958/0974-360X.2019.00577.8 Available on: https://rjptonline.org/AbstractView.aspx?PID=2019-12-7-52