Author(s): Maha A. Hegazy, Amr M. Badawey, Maryam A. Bakr, Samah S. Abbas

Email(s): mariam.bakr@fue.edu.eg

DOI: 10.5958/0974-360X.2019.00443.8   

Address: Maha A. Hegazy1, Amr M. Badawey1,2, Maryam A. Bakr2*, Samah S. Abbas1
1Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., 11562 Cairo, Egypt
2Pharmaceutical Chemistry Department, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt, 90th St., Fifth Settlement, New Cairo, 11835 Cairo, Egypt
*Corresponding Author

Published In:   Volume - 12,      Issue - 6,     Year - 2019


ABSTRACT:
Four spectrophotometric methods were developed and validated for spectral resolution and determination of thioctic acid (THC), benfotiamine (BEN) and cyanocobalamin (CNCo) in their pure form, laboratory prepared mixtures and capsules. Method A, a first derivative method for determination of CNCo, by recording the peak amplitude at 564.0 nm. Method B, is a Ratio difference spectrophotometric one which is applied for determination of THC and BEN, where CNCo has no interference. Method C applied first derivative of ratio spectra where THC and BEN were determined by recording the peak amplitude at 330.8 and 286.8 nm, in order. Method D a Ratio subtraction method that was successfully determines BEN at its ?max without interference of the other co-formulated drugs. Validation according to ICH guidelines was performed. The linearity ranges were 200.00-1800.00, 2.00-30.00 and 10.00-200.00 µg/mL for THC, BEN and CNCo, in a respective order. The methods were found to be accurate, precise and specific.


Cite this article:
Maha A. Hegazy, Amr M. Badawey, Maryam A. Bakr, Samah S. Abbas. Spectrophotometric Resolution and Quantification of Ternary Co-formulated Mixture of Thioctic acid, Benfotiamine and Cyanocobalamin. Research J. Pharm. and Tech. 2019; 12(6): 2648-2656. doi: 10.5958/0974-360X.2019.00443.8


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 


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