Author(s): Muhammad Isrul, Risky Juliansyah, Ahmad Saleh, Wa Ode Yuliastri, Jastria Pusmarani, Himaniarwati, Wa Ode Wahyuni Maulidina

Email(s): isrulfar@gmail.com

DOI: 10.5958/0974-360X.2019.00329.9   

Address: Muhammad Isrul*, Risky Juliansyah, Ahmad Saleh, Wa Ode Yuliastri, Jastria Pusmarani, Himaniarwati, Wa Ode Wahyuni Maulidina
Department of Pharmacy, STIKES Mandala Waluya Kendari, South-East Sulawesi, Indonesia
*Corresponding Author

Published In:   Volume - 12,      Issue - 4,     Year - 2019


ABSTRACT:
Last several years, researches about the plant as an alternative treatment of breast cancer have been developed. Curcuma aureginosa has been applied empirically as a breast cancer treatment and provide a satisfying result. The aim of this study is to examine cytotoxic activity of C.aureginosa extract to MCF-7 breast cancer cells, phytochemical analysis, and standardization of extract. Examination of breast cancer activity conducted by MTT method, extract was examined at concentration of 2000, 1000, 500, 250, 125, 62.5, 32.25, 15.625, and 7.8125 µg/ml, doxorubicin 1 µg/ml is applied as a positive control. Phytochemical Analysis conducted by GC-MS, and standardization of extract conducted based on Indonesia Pharmacopeia. The results reveal that extract has cytotoxic activity toward MCF-7 breast cancer cell. Concentration of 2000, 1000, 500, 250µg/ml has similar activity with doxorubicin 1 µg/ml. IC50 value of the extract is 76.24 µg/ml with 48 hours incubation. Standardization result determines extract profile and completes the standard of Indonesia Pharmacopeia. Phytochemical analysis result reveals Germacra-1(10), 4-diene-12-oic acid 6 alpha hydroxy gamma lactone (25.12%) and Cycloisolongifolene, 8, 9-dehydro-9-formyl- (21.61%) as a major compound. Cycloisolongifolene, 8, 9-dehydro-9-formyl-, Alpha-Humulene, Curcumene, and Curzerene assumed as cytotoxic toward MCF-7 breast cancer cell.


Cite this article:
Muhammad Isrul, Risky Juliansyah, Ahmad Saleh, Wa Ode Yuliastri, Jastria Pusmarani, Himaniarwati, Wa Ode Wahyuni Maulidina. Phytochemical Analysis, Standardization and Cytotoxic Activity of Curcuma aureginosa Extract in Human Breast Cancer (MCF-7) Cell Line. Research J. Pharm. and Tech. 2019; 12(4): 1967-1973. doi: 10.5958/0974-360X.2019.00329.9

Cite(Electronic):
Muhammad Isrul, Risky Juliansyah, Ahmad Saleh, Wa Ode Yuliastri, Jastria Pusmarani, Himaniarwati, Wa Ode Wahyuni Maulidina. Phytochemical Analysis, Standardization and Cytotoxic Activity of Curcuma aureginosa Extract in Human Breast Cancer (MCF-7) Cell Line. Research J. Pharm. and Tech. 2019; 12(4): 1967-1973. doi: 10.5958/0974-360X.2019.00329.9   Available on: https://rjptonline.org/AbstractView.aspx?PID=2019-12-4-84


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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