Author(s): Yeragodala Narendra Reddy, Jadi Sreeramulu, B Balaswami

Email(s): skuynr@gmail.com

DOI: 10.5958/0974-360X.2019.00111.2   

Address: Yeragodala Narendra Reddy, Jadi Sreeramulu*, B Balaswami
Department of Chemistry, Sri Krishnadevaraya University, Ananthapuramu-515003 A.P, India.
*Corresponding Author

Published In:   Volume - 12,      Issue - 2,     Year - 2019


ABSTRACT:
A simple, accurate, precise method was developed for the simultaneous estimation of the Glecaprevir and Pibrentasvir in tablet dosage form. Chromatogram was run through Std Zodiac 150 x 4.6 mm, 5m. Mobile phase containing buffer 0.1% OPA: Acetonitrile taken in the ratio 55:45 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 260 nm. Retention time of Glecaprevir and Pibrentasvir were found to be 2.207 min and 3.263 min. %RSD of the Glecaprevir and Pibrentasvir were and found to be 0.4 and 0.3 respectively. %Recovery was obtained as 98.90% and 99.61% for Glecaprevir and Pibrentasvir respectively. LOD, LOQ values obtained from regression equations of Glecaprevir and Pibrentasvir were 0.32, 0.97 and 0.04, 0.11 respectively. Regression equation of Glecaprevir is y= 23713.x + 11307, y = 13524x +2221 of Pibrentasvir. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular quality control test in industries.


Cite this article:
Yeragodala Narendra Reddy, Jadi Sreeramulu, B Balaswami. A New Stability- Indicating RP-HPLC- Pda Method for Simultaneous Estimation of Glecaprevir and Pibrentasvir in tablet dosage form. Research J. Pharm. and Tech 2019; 12(2):625-631. doi: 10.5958/0974-360X.2019.00111.2

Cite(Electronic):
Yeragodala Narendra Reddy, Jadi Sreeramulu, B Balaswami. A New Stability- Indicating RP-HPLC- Pda Method for Simultaneous Estimation of Glecaprevir and Pibrentasvir in tablet dosage form. Research J. Pharm. and Tech 2019; 12(2):625-631. doi: 10.5958/0974-360X.2019.00111.2   Available on: https://rjptonline.org/AbstractView.aspx?PID=2019-12-2-30


Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available