Palak Chaudhary, Harsha U. Patel
Dr Palak Chaudhary*, Dr. Harsha U. Patel
Shri Satsangi Saketdham “Ram Ashram”, Group of Institutions, Faculty of Pharmacy,
Vadasma, Mehsana, Gujarat, India.
Volume - 12,
Issue - 12,
Year - 2019
The present study was focused on establishing and validating a simple, accurate, reliable and economical UV spectrophotometric method for the simultaneous determination of Rutin trihydrate (RUT) and Berberine Chloride (BER) in synthetic mixture and in antidiabetic polyherbal formulation. Rutin and Berberine from herbal plants are used as antidiabetic drug to prevent and manage Diabetes. The developed method gives best results in terms of linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). The method was validated as per ICH guidelines. The linearity range for Rutin trihydrate and Berberine Chloride were found to be 10 to 50 µg/ ml and 10 to 50 µg/ml respectively. The values of LOD were 0.062 µg/ ml and 0.076 µg/ml and the values of LOQ were found to be 0.187 µg /ml and 0.229 µg/ ml for Rutin trihydrate and Berberine chloride respectively.
Cite this article:
Palak Chaudhary, Harsha U. Patel. Simultaneous Equation Method for Estimation of Rutin Trihydrate and Berberine Chloride in Synthetic Mixture and Polyherbal Formulation. Research J. Pharm. and Tech. 2019; 12(12): 5811-5814. doi: 10.5958/0974-360X.2019.01006.0