ABSTRACT:
Aim:The present study is to degrade the engine oil through microbes from the industrialized areas in and around Chrompet, Chennai. Engine oil contaminated soil samples were collected from different locations of Chrompet which is located 35 KM away from Chennai. Methods:As a result of spread plate technique the microbial colonies were enumerated and three different organisms such as bacteria namely Pseudomonas sp and Bacillus sp.Actinomycetesnamely Streptomyces spand funginamely Aspergillus flavus and Aspergillus nigerwereidentified. The bacterial colonies were identified by morphological and biochemical techniques using the taxonomic scheme. pH and optical density (growth rate) was determined for Pseudomonas sp, Bacillus sp, consortium (Pseudomonas sp and Bacillus sp), Streptomyces sp,Aspergillusflavus and Aspergillus nigerto the concentration from 1ml to 5ml for 16 days. Results:All the isolated microorganisms had the potential to degrade the engine oil. Comparatively the bacterial consortium had the highest potential to degrade the engine oil.Summary:This formulation may be the best solution to solve the land pollution by engine oil and also to conserve the natural environment.