Author(s): Mustafa Salah Hasan, Mohammed Ali Hussein, Afaf Abdulrahman Yousif

Email(s): drmustafasalah7@uofallujah.edu.iq

DOI: 10.5958/0974-360X.2019.00046.5   

Address: Mustafa Salah Hasan1, Mohammed Ali Hussein1, Afaf Abdulrahman Yousif2
1Department of Internal and Preventive Vet. Medicine, College of Vet. Med., University of Fallujah, Iraq
2Department of Internal and Preventive Vet. Medicine, College of Vet. Med., University of Baghdad, Iraq
*Corresponding Author

Published In:   Volume - 12,      Issue - 1,     Year - 2019


ABSTRACT:
Shiga toxigenic E. coli O157:H7 is one of the Shiga-toxin producing types of E. coli, which can produce disease in man and animals. The aims of this study were to detect O gene (rfbO157) and H gene( fliCH7) and determine the pattern of antimicrobial susceptibility of 32 E. coli O157:H7 isolates. The isolates isolated by conventional culture methods and latex test from faecal samples of 4 calves suffering from diarrhoea and 28 calves apparent normal. DNA was extracted from these isolates and the real time PCR was applied on the extracted DNA for confirming the diagnosis of rfbO157 and flic H7 genes. Seven antimicrobial discs were used in this study. The result presented that all the (32) isolates were possessed the rfbO157 and flics H7 genes. The susceptibility test of the isolates revealed that all isolates were highly resistant to Erythromycin (100%) and Cephalothin (96.87%). Less resistance to Tetracycline, Trimethoprim-Sulfamethoxazole and Chloramphenicol at different percentage (31.25%), (12.5%) and (9.37%) respectively and all isolates showed high sensitivity to Gentamicin (100%), Enrofloxacin (100%). In conclusion, the real time PCR can be used as a sensitive and reliable tool for diagnosis of E. coli O157:H7, and this bacteria showed high resistance to antibiotic.


Cite this article:
Mustafa Salah Hasan, Mohammed Ali Hussein, Afaf Abdulrahman Yousif. Confirmatory Detection of Escherichia coli O157:H7 in Diarrhoeic and Non-Diarrhoeic Calves by using real time PCR with Studying the Antimicrobial Susceptibility of these bacteria. Research J. Pharm. and Tech 2019; 12(1): 245-250. doi: 10.5958/0974-360X.2019.00046.5


Recomonded Articles:

Author(s): Chinedu Fredrick Anowi, Nnabuife Chinedu Cardinal, A.F. Onyegbule, L.N. Anowi

DOI: Not Available         Access: Open Access Read More

Author(s): Saurabh Bharadwaj, U.V.S. Teotia, Kishan Singh, Rajib Sharma, Yogendra Singh

DOI: Not Available         Access: Open Access Read More

Author(s): Roushan Mubarak, Shaden Haddad, Outhman Hamdan

DOI: 10.5958/0974-360X.2016.00022.6         Access: Open Access Read More

Author(s): G. Bhuvaneshwari, . A. S. Shameembanu, M. Kalyani

DOI: 10.5958/0974-360X.2018.00244.5         Access: Open Access Read More

Author(s): Nadheema Hammood Hussein, Baydaa Hussein Alwan, Buthainah Mohammed Taha, Sinaa Mahdi Shakir, Ban Mahdi Shakir

DOI: 10.5958/0974-360X.2019.00676.0         Access: Open Access Read More

Author(s): S. Ramachandran, Vijey Aanandhi M, Binoy Varghese Cheriyan

DOI: 10.5958/0974-360X.2019.00684.X         Access: Open Access Read More

Author(s): Abdullahi Rabiu Abubakar, Nordin Bin Simbak, Mainul Haque

DOI: Not Available         Access: Open Access Read More

Author(s): Haytham Yazigi, Atiya khamees, Houda Wakil

DOI: 10.5958/0974-360X.2019.00209.9         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags