ABSTRACT:
The current research is focused on phytochemical screening of ethanolic extract of S. cumini seeds by biochemical and analytical methods (GCMS) and determination of antimicrobial activity of the same extract against major food borne pathogens. Both biochemical and GC-MS analysis revealed the existence of bioactive molecules such as flavonoids, alkaloids, saponins, tannins, steroids, terpenoids, resins etc. The antimicrobial action of the ethanolic extract (20%, 40%, 60%, 80% and 100%) of Jamun seeds were done againstfoodborne pathogens such asStreptococcus agalactiae, Staphylococcus aureus, Salmonella Typhimurium, Escherichia coli, Pseudomonas aeruginosa, Klebsiellapneumoniae, Bacillus cereus, Enterococcus faecalis, Clostridium perfringens, Listeria monocytogenes, Aeromonashydrophilaby disc diffusion method. The value obtained from this provide us the level at which the pathogens shows susceptibility for the S.cumini extract. The results prove that, Bacillus cereus was more sensitive and Staphylococcus aureus, Clostridium perfringens, Aeromonas2hydrophila were less susceptible to the extract. The Antioxidant assay was done by using invitro DPPH free radicals scavenging assay.