Author(s): Ola Mahmoud Younes, Fida Am Ali, Zaid Al Assaf

Email(s): ola-younes1@hotmail.com

DOI: 10.5958/0974-360X.2018.00723.0   

Address: Ola Mahmoud Younes1*, Fida Am Ali2, Zaid Al Assaf3
1PhD Student, Department of analytical and food chemistry, Faculty of Pharmacy, Damascus University, Damascus, Syria
2Professor-Assistant, Department of analytical and food chemistry, Faculty of of Pharmacy, Damascus University, Damascus, Syria
3Professor, Department of analytical and food chemistry, Faculty of Pharmacy, Damascus University, Damascus, Syria
*Corresponding Author

Published In:   Volume - 11,      Issue - 9,     Year - 2018


ABSTRACT:
Enantiomeric separation of metoprolol tartrate was developed using a reversed phase HPLC (RP-HPLC) with methyl-??-cyclodextrin (M-??-CD) as a chiral mobile phase additive. The effects of the pH value of mobile phase, concentration of chiral additive, composition of mobile phase on the enantioseparation of metoprolol tartrate were investigated on Macherey Nagel C18 column (250 mm×4.6 mm, 5.0 µm). The detection was performed using UV at 274 nm. A satisfied resolution was achieved using a mobile phase consists of a mixture of aqueous solution( 1.5g/l M-??-CD), and methanol with a volumetric ratio of (86/14)v/v%, and flow rate of 0.5 mL/min. The linearity, precision and accuracy were studied to prove the validity of the developed method and could simply applied to determine the enantiomers of metoprolol tartrate in pharmaceutical tablets.


Cite this article:
Ola Mahmoud Younes, Fida Am Ali, Zaid Al Assaf. Enantioseparation of Metoprolol Tartrate using HPLC by Adding Methyl beta Cyclodextrin to the mobile Phase (As Chiral Additive). Research J. Pharm. and Tech 2018; 11(9): 3937-3942. doi: 10.5958/0974-360X.2018.00723.0

Cite(Electronic):
Ola Mahmoud Younes, Fida Am Ali, Zaid Al Assaf. Enantioseparation of Metoprolol Tartrate using HPLC by Adding Methyl beta Cyclodextrin to the mobile Phase (As Chiral Additive). Research J. Pharm. and Tech 2018; 11(9): 3937-3942. doi: 10.5958/0974-360X.2018.00723.0   Available on: https://rjptonline.org/AbstractView.aspx?PID=2018-11-9-38


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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