Author(s): Pramadvara Kallepalli, Mukthinuthalapati Mathrusri Annapurna

Email(s): pramadvarakallepalli4@gmail.com

DOI: 10.5958/0974-360X.2018.00672.8   

Address: Pramadvara Kallepalli*, Mukthinuthalapati Mathrusri Annapurna
Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Visakhapatnam-530045, India
*Corresponding Author

Published In:   Volume - 11,      Issue - 8,     Year - 2018


ABSTRACT:
Gefitinib, an anticancer drug and its process related impurities were estimated using WATERS 2695 Series HPLC system equipped with Quaternary gradient pumps and WATERS 2996 PDA detector with EMPOWER software. ESI-QTOF- MS system with Waters Quattro premier XE triple quadrupole with Acquity HPLC system with Masslynx software was used for the mass spectral studyof the impurities. Reverse phase C-18 column-Inertsil ODS 3V (150 X 4.6 mm, 5µm particle) was used and the total run time was 45 min. Gefitinib was subjected to forced degradation studies and the degradant products were analyzed using mass spectroscopy. Impurities (I - X) were separated on isocratic mode and calibrated using mass spectroscopy. Gefitinib was found to be more sensitive towards oxidation and three degradants were observed and their mass spectra also analyzed. The method was validated (ICH guidelines).


Cite this article:
Pramadvara Kallepalli, Mukthinuthalapati Mathrusri Annapurna. Separation, Identification and Quantification of process related Impurities and Stress Degradants of Gefitinib by LC-ESI-Q–TOF/MS. Research J. Pharm. and Tech 2018; 11(8): 3647-3657. doi: 10.5958/0974-360X.2018.00672.8

Cite(Electronic):
Pramadvara Kallepalli, Mukthinuthalapati Mathrusri Annapurna. Separation, Identification and Quantification of process related Impurities and Stress Degradants of Gefitinib by LC-ESI-Q–TOF/MS. Research J. Pharm. and Tech 2018; 11(8): 3647-3657. doi: 10.5958/0974-360X.2018.00672.8   Available on: https://rjptonline.org/AbstractView.aspx?PID=2018-11-8-77


Recomonded Articles:

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): Peethala Prathyusha, Raja Sundararajan, Palyam Bhanu, Mathrusri Annapurna Mukthinuthalapati

DOI: 10.5958/0974-360X.2020.00507.7         Access: Open Access Read More

Author(s): V. Shirisha, Somsubhra Ghosh, B. Rajni, David Banji

DOI: Not Available         Access: Open Access Read More

Author(s): Narendra Chotai, Vishnu Patel, Harsha Patel, Uren Patel, Rajendra Kotadiya

DOI:         Access: Open Access Read More

Author(s): R. P. Bhole, S. R. Jagtap, K. B. Chadar, Y. B. Zambare

DOI: 10.5958/0974-360X.2020.00189.4         Access: Open Access Read More

Author(s): Shrivastava Alankar, Jain R., Agrawal R.K., Ahirwar D.

DOI:         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Vijaykumar T. Pawar, Shubhangi V. Pawar, Harinath N. More, Anita S. Kulkarni, Dinanath T. Gaikwad

DOI: 10.5958/0974-360X.2017.00724.7         Access: Open Access Read More

Author(s): Mukta D. Naykode, Durgacharan A. Bhagwat, Swapnil D. Jadhav, Harinath N. More

DOI: 10.5958/0974-360X.2017.00133.0         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags