ABSTRACT:
Objective:To explore intertidal zone Swell seawater (High tide) for presence of novel Halotolerant Marine Actinomycetes (HTMA). Methods: Sea water analysis performed to estimate physiochemical parameters of unique Habitat. Phenotypic and genotypic analysis of isolated Halotolerant Marine Actinomycetes strains. Bioinformatics based analysis of novel HTMA Strain AMET YTC_21. Results: Physiochemical parameters of samples were as Temperature Seawater (28.00±0.50)°C, Salinity (34.96±0.05) ppt, Electrical Conductivity (34.96±0.05) µs/cm, pH (8.88±0.084), Dissolved Oxygen (11.94±0.11) mg/L, NO3 (0.25±0.021) mg/L, Total Dissolved Solids (27850.2±0.44) mg/L and Total PO4(0.013±0.00)mg/L. Salt tolerance, pH and temperature tolerance of selected HTMA strains and selection of potential strain for genetic analysis. Bioinformatics based Homology modeling and Phylogenetic tree analysis of novel Streptomyces sp. AMET_YTC21 strain. Conclusion: In the present study molecular identification by 16s rRNA gene sequencing of the isolated HTMA strain confirmed the organism a streptomyces sp. AMET_YTC21. The 16s rRNA nucleotide sequence was submitted to Gen bank in NCBI and the accession number obtained as Bank It KY072795. Morphological and biochemical evidence also suggested the presence of novel strain with high pH, Salt and temperature tolerance as Halotolerant Marine Actinomycetes. The obtained results will help us to determine the right candidate for novel therapeutic enzyme production.