ABSTRACT:
Poly (lactic acid) (PLA) has become one of the most concerned polymer as biodegradable and biocompatible material. PLA has large application that covers many areas but the most interest field are biomedical and biopharmaceutical thanks to the characteristic of biocompatible. In the present work, PLA was synthesized from the supernatant of Lactobacillus rhamnosus PN04 in different culturing conditions to obtain high molecular weight. As a result, the pH7 and the incubation at 37oC are the best culturing condition in aid of combination of xylene and reduced pressure agent. The molecular weight achieved was around 11000 g/mole which were characterized by thin layer chromatography (TLC), infrared spectroscopy (IR), nuclear magnetic resonance (H-NMR), gel permeation chromatography (GPC) and scanning electron microscope (SEM). Moreover, the polymer made from L. rhamnosus also having the advantage differently from the conventional one that are antimicrobial against the three tested species as Candida albicans, Pseudomonas aeruginosa and Micrococcus luteus and cytotoxicity activity against HepG2. PLA would be used in pharmaceutical science.