ABSTRACT:
Background/Objectives: This study aims to demonstrate pro-inflammatory cytokine production inhibitory effects using everlasting oil. Methods/Statistical analysis:Thus, This paper was carried out to see secretion of NO, inflammatory cytokine ,TNF-a , IL-1ß using the macrophages of LPS-treated mice. The cytotoxicity test was used 96 AQueous One solution cell proliferation assay. No Detection kit was used to measure No ,the production of pro-inflammatory cytokine was measured using ELISA kit. Findings: As a result, everlasting oil had no cytotoxicity at 5 ug/mL to 500 ug/mL and significantly inhibited the production of NO as well as TNF-a, IL-1ß. In the RAW 264.7 mousemacrpphage cells,Pro-inflammatory cytokine production inhibitory effects are likely to be diversely utilized as basic physiological activity data and functional materials to demonstrate anti-inflammatory properties of everlasting oil. Improvements/Applications: In conclusion, this study can be used to a basic data to objectively demonstrate the physiological activity of immunological mechanism associated with the anti-inflammatory action of everlasting oil, but it is necessary to conduct studies on anti-inflammation.