Author(s): Manickavelu Manimaran, Krishnan Kannabiran

Email(s): kkb@vit.ac.in

DOI: 10.5958/0974-360X.2018.00403.1   

Address: Manickavelu Manimaran, Krishnan Kannabiran*
Department of Bio-Medical Sciences, School of Biosciences and Technology, Vellore Institute of Technology, Vellore-632014, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 11,      Issue - 6,     Year - 2018


ABSTRACT:
The objective of this study is to screen for the a-Amylase activity of a marine actinomycete strain Streptomyces sp. VITMK1 isolated from soil sediment sample collected from the mangrove region of Pichavaram, Tamil Nadu, India. Streptomyces sp. VITMK1 was isolated from soil sediment sample collected from the mangrove region of Pichavaram. The isolate was subjected to submerged fermentation in tryptone yeast extract (ISP No.1) medium and after the optimal duration, the cell-free supernatant was collected by centrifugation and used to check a-Amylase activity by 3,5 dinitrosalicylic acid (DNSA) method. A standard maltose curve was used to estimate the amount of maltose present in the sample after the reaction. After five minutes of assay conditions, 160 µg/ml of maltose was released by the activity of a-Amylase produced by the strain Streptomyces sp. VITMK1 and the enzyme activity was calculated as 0.934 U. The results of the study revealed that actinomycetes are a potent source for extracellular a-Amylase production. By optimizing the reaction conditions the enzyme activity of a-Amylase could be substantially increased.


Cite this article:
Manickavelu Manimaran, Krishnan Kannabiran. Alpha amylase activity of Streptomyces sp. VITMK1 isolated from marine soil sample of Pichavaram, Tamil Nadu, India.Research J. Pharm. and Tech 2018; 11(6): 2180-2182. doi: 10.5958/0974-360X.2018.00403.1

Cite(Electronic):
Manickavelu Manimaran, Krishnan Kannabiran. Alpha amylase activity of Streptomyces sp. VITMK1 isolated from marine soil sample of Pichavaram, Tamil Nadu, India.Research J. Pharm. and Tech 2018; 11(6): 2180-2182. doi: 10.5958/0974-360X.2018.00403.1   Available on: https://rjptonline.org/AbstractView.aspx?PID=2018-11-6-4


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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