ABSTRACT:
A unique and speedy colorimetric method has been developed and validated for the estimation of Raltegravir in pharmaceutical formulation and human biological fluids using 3-methyl-2-benzothiazolinone hydrazone (MBTH) reagent. The projected technique is based on oxidative coupling reaction of MBTH reagent with Raltegravir by using ferric chloride solution and HCl to produce bluish green chromogen at 572nm. The linearity was established in the range of 80-220 µg/mL with regression equation 0.004x+0.007 having r2=0.999. The method shows good precision and ruggedness with accuracy in between 99.85%-100.43% with % RSD less than 2%. The optical characteristics and various statistical reports were reported successfully and there was no interference of any excipients and reagents in this method. The method which was depicted in this research work was successfully applied and the results are compared with the reference standards and satisfactory validated results were obtained.